RegulonDB RegulonDB 10.9: Gene Form
   

treF gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

treF yhjB nc8 ccp OxyR OxyR FNR TSS_4131 TSS_4131 treFp treFp yhjAp yhjAp

Gene      
Name: treF    Texpresso search in the literature
Synonym(s): ECK3504, EG12245, b3519
Genome position(nucleotides): 3669592 --> 3671241 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.94
External database links:  
ASAP:
ABE-0011497
CGSC:
35861
ECHOBASE:
EB2156
ECOLIHUB:
treF
OU-MICROARRAY:
b3519
STRING:
511145.b3519
COLOMBOS: treF


Product      
Name: cytoplasmic trehalase
Synonym(s): TreF
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 63.697
Isoelectric point: 4.673
Motif(s):
 
Type Positions Sequence
221 -> 223 RSQ
175 -> 176 WD
70 -> 546 KIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLY
292 -> 294 RDE

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
molecular_function GO:0016787 - hydrolase activity
GO:0016798 - hydrolase activity, acting on glycosyl bonds
GO:0004555 - alpha,alpha-trehalase activity
biological_process GO:0008152 - metabolic process
GO:0005975 - carbohydrate metabolic process
GO:0005991 - trehalose metabolic process
GO:0005993 - trehalose catabolic process
GO:0071474 - cellular hyperosmotic response
Note(s): Note(s): ...[more].
Reference(s): [1] Uhland K., et al., 2000
External database links:  
ECOCYC:
TREHALACYTO-MONOMER
ECOLIWIKI:
b3519
ECOO157CYC:
TREF-MONOMER
INTERPRO:
IPR023715
INTERPRO:
IPR001661
INTERPRO:
IPR008928
INTERPRO:
IPR012341
INTERPRO:
IPR018232
PANTHER:
PTHR23403
PFAM:
PF01204
PRIDE:
P62601
PRINTS:
PR00744
PRODB:
PRO_000024109
PROSITE:
PS00927
PROSITE:
PS00928
REFSEQ:
NP_417976
SMR:
P62601
UNIPROT:
P62601


Operon      
Name: treF         
Operon arrangement:
Transcription unit        Promoter
treF


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4130 3668779 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_4131 3670505 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Uhland K., Mondigler M., Spiess C., Prinz W., Ehrmann M., 2000, Determinants of translocation and folding of TreF, a trehalase of Escherichia coli., J Biol Chem 275(31):23439-45

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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