RegulonDB RegulonDB 10.9: Gene Form
   

bcsE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

bcsG bcsE bcsF yhjR anti-terminator terminator TSS_4139 TSS_4139 bcsEp2 bcsEp2 TSS_4137 TSS_4137 bcsEp11 bcsEp11 yhjRp13 yhjRp13 yhjRp11 yhjRp11 yhjRp yhjRp yhjRp5 yhjRp5 bcsEp8 bcsEp8 yhjRp10 yhjRp10 yhjRp12 yhjRp12

Gene      
Name: bcsE    Texpresso search in the literature
Synonym(s): ECK3521, EG12263, b3536, yhjS
Genome position(nucleotides): 3696458 --> 3698029 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
48.85
External database links:  
ASAP:
ABE-0011554
ECHOBASE:
EB2172
ECOLIHUB:
bcsE
OU-MICROARRAY:
b3536
STRING:
511145.b3536
COLOMBOS: bcsE


Product      
Name: c-di-GMP-binding protein BcsE
Synonym(s): BcsE, YhjS
Sequence: Get amino acid sequence Fasta Format
Molecular weight: 59.428
Isoelectric point: 6.576
Motif(s):
 
Type Positions Sequence
139 -> 139 R
306 -> 306 R
364 -> 364 R
6 -> 514 DPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLANQTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCSIDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSLLLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRIPEPMRL
287 -> 287 R

 

Classification:
Multifun Terms (GenProtEC)  
Gene Ontology Terms (GO)  
molecular_function GO:0000166 - nucleotide binding
GO:0035438 - cyclic-di-GMP binding
biological_process GO:0030244 - cellulose biosynthetic process
Note(s): Note(s): ...[more].
Reference(s): [1] Acheson JF., et al., 2019
[2] Fang X., et al., 2014
External database links:  
DIP:
DIP-12390N
ECOCYC:
EG12263-MONOMER
ECOLIWIKI:
b3536
INTERPRO:
IPR017745
PFAM:
PF10995
PRIDE:
P37657
REFSEQ:
NP_417993
UNIPROT:
P37657


Operon      
Name: bcsEFG         
Operon arrangement:
Transcription unit        Promoter
bcsEFG


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yhjRp12 3696215 reverse Similarity to the consensus
Read more >
[ICWHO] [3]
  promoter yhjRp10 3696283 reverse Similarity to the consensus
Read more >
[ICWHO] [3]
  promoter bcsEp8 3696322 forward Similarity to the consensus
Read more >
[ICWHO] [3]
  promoter yhjRp5 3696356 reverse Similarity to the consensus
Read more >
[ICWHO] [3]
  promoter yhjRp11 3696367 reverse Similarity to the consensus
Read more >
[ICWHO] [3]
  promoter yhjRp13 3696375 reverse Similarity to the consensus
Read more >
[ICWHO] [3]
  promoter bcsEp11 3696395 forward Similarity to the consensus
Read more >
[ICWHO] [3]
  promoter TSS_4137 3696397 forward nd [RS-EPT-CBR] [4]
  promoter TSS_4139 3699265 forward nd [RS-EPT-CBR] [4]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Acheson JF., Derewenda ZS., Zimmer J., 2019, Architecture of the Cellulose Synthase Outer Membrane Channel and Its Association with the Periplasmic TPR Domain., Structure 27(12):1855-1861.e3

 [2] Fang X., Ahmad I., Blanka A., Schottkowski M., Cimdins A., Galperin MY., Romling U., Gomelsky M., 2014, GIL, a new c-di-GMP-binding protein domain involved in regulation of cellulose synthesis in enterobacteria., Mol Microbiol 93(3):439-52

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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