RegulonDB RegulonDB 10.8: Gene Form
   

eamB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

yfiE eamB grcA TSS_2879 (cluster) TSS_2879 (cluster) TSS_2878 (cluster) TSS_2878 (cluster) TSS_2877 TSS_2877 TSS_2876 TSS_2876 TSS_2875 TSS_2875 yfiEp7 yfiEp7

Gene      
Name: eamB    Texpresso search in the literature
Synonym(s): ECK2576, EG12445, b2578, yfiK
Genome position(nucleotides): 2715423 --> 2716010 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.19
External database links:  
ASAP:
ABE-0008487
ECHOBASE:
EB2339
OU-MICROARRAY:
b2578
PortEco:
eamB
STRING:
511145.b2578
COLOMBOS: eamB


Product      
Name: cysteine/O-acetylserine exporter EamB
Synonym(s): EamB, YfiK
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 21.248
Isoelectric point: 9.984
Motif(s):
 
Type Positions Sequence
105 -> 125 ISFWASFALQFVNVKIILYGV
8 -> 28 AFWTYTLITAMTPGPNNILAL
70 -> 90 AAVHLLSWAGAAYIVWLAWKI
47 -> 67 SLGFLIVMLLCAGISFSLAVI
142 -> 162 VVGVSVLLAMIGTFGNVCWAL

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015171 - amino acid transmembrane transporter activity
GO:0015562 - efflux transmembrane transporter activity
GO:0033229 - cysteine transmembrane transporter activity
biological_process GO:0006865 - amino acid transport
GO:0033228 - cysteine export across plasma membrane
Note(s): Note(s): ...[more].
Reference(s): [1] Aleshin VV., et al., 1999
[2] Franke I., et al., 2003
External database links:  
ECOCYC:
EG12445-MONOMER
ECOLIWIKI:
b2578
INTERPRO:
IPR001123
PANTHER:
PTHR30086
PFAM:
PF01810
PRIDE:
P38101
PRODB:
PRO_000022496
REFSEQ:
NP_417073
UNIPROT:
P38101


Operon      
Name: eamB         
Operon arrangement:
Transcription unit        Promoter
yfiK


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter yfiEp7 2715344 reverse Similarity to the consensus
Read more >
[ICWHO] [3]
  promoter TSS_2875 2716280 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2876 2716282 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2877 2716291 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_2878 (cluster) 2716431 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [4]
  promoter TSS_2879 (cluster) 2716446 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [4]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Aleshin VV., Zakataeva NP., Livshits VA., 1999, A new family of amino-acid-efflux proteins., Trends Biochem Sci 24(4):133-5

 [2] Franke I., Resch A., Dassler T., Maier T., Bock A., 2003, YfiK from Escherichia coli promotes export of O-acetylserine and cysteine., J Bacteriol 185(4):1161-6

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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