RegulonDB RegulonDB 10.8: Gene Form
   

mdtM gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

yjiR yjiN mdtM rpnD TSS_5146 TSS_5146

Gene      
Name: mdtM    Texpresso search in the literature
Synonym(s): ECK4328, EG12576, b4337, yjiO
Genome position(nucleotides): 4567287 <-- 4568519 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.77
External database links:  
ASAP:
ABE-0014221
ECHOBASE:
EB2464
OU-MICROARRAY:
b4337
PortEco:
mdtM
STRING:
511145.b4337
COLOMBOS: mdtM


Product      
Name: multidrug efflux pump / bile salt:H+ antiporter / Na+:H+ antiporter / K+:H+ antiporter
Synonym(s): MdtM, MdtM drug MFS transporter, YjiO, multidrug efflux pump MdtM
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 44.688
Isoelectric point: 10.613
Motif(s):
 
Type Positions Sequence
49 -> 69 LAPAAVSLYLAGGMALQWLLG
308 -> 328 VWLWSVLGTSLYAFGIGLIFP
342 -> 342 K
26 -> 26 Y
128 -> 128 E

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0005623 - cell
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0015125 - bile acid transmembrane transporter activity
GO:0022857 - transmembrane transporter activity
GO:0015299 - solute:proton antiporter activity
GO:0015385 - sodium:proton antiporter activity
GO:0042910 - xenobiotic transmembrane transporter activity
GO:0015386 - potassium:proton antiporter activity
biological_process GO:0097623 - potassium ion export across plasma membrane
GO:1990961 - xenobiotic detoxification by transmembrane export across the plasma membrane
GO:0036376 - sodium ion export across plasma membrane
GO:0046677 - response to antibiotic
GO:0030641 - regulation of cellular pH
GO:0006855 - drug transmembrane transport
GO:0055085 - transmembrane transport
GO:0015721 - bile acid and bile salt transport
GO:0098656 - anion transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Alegre KO., et al., 2015
[2] Alon Cudkowicz N., et al., 2019
[3] Edgar R., et al., 1997
[4] Jindal S., et al., 2019
[5] Schuldiner S. 2018
[6] Shuster Y., et al., 2016
[7] Tal N., et al., 2009
External database links:  
DIP:
DIP-12641N
ECOCYC:
YJIO-MONOMER
ECOLIWIKI:
b4337
INTERPRO:
IPR011701
INTERPRO:
IPR036259
INTERPRO:
IPR020846
INTERPRO:
IPR005829
MODBASE:
P39386
PFAM:
PF07690
PRIDE:
P39386
PRODB:
PRO_000023193
PROSITE:
PS50850
REFSEQ:
NP_418757
SMR:
P39386
UNIPROT:
P39386


Operon      
Name: mdtM-yjiN         
Operon arrangement:
Transcription unit        Promoter
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_5146 4569651 forward nd [RS-EPT-CBR] [8]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Alegre KO., Law CJ., 2015, Purification of a Multidrug Resistance Transporter for Crystallization Studies., Antibiotics (Basel) 4(1):113-35

 [2] Alon Cudkowicz N., Schuldiner S., 2019, Deletion of the major Escherichia coli multidrug transporter AcrB reveals transporter plasticity and redundancy in bacterial cells., PLoS One 14(6):e0218828

 [3] Edgar R., Bibi E., 1997, MdfA, an Escherichia coli multidrug resistance protein with an extraordinarily broad spectrum of drug recognition., J Bacteriol 179(7):2274-80

 [4] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [5] Schuldiner S., 2018, The Escherichia coli effluxome., Res Microbiol 169(7-8):357-362

 [6] Shuster Y., Steiner-Mordoch S., Alon Cudkowicz N., Schuldiner S., 2016, A Transporter Interactome Is Essential for the Acquisition of Antimicrobial Resistance to Antibiotics., PLoS One 11(4):e0152917

 [7] Tal N., Schuldiner S., 2009, A coordinated network of transporters with overlapping specificities provides a robust survival strategy., Proc Natl Acad Sci U S A 106(22):9051-6

 [8] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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