RegulonDB RegulonDB 10.8: Gene Form
   

rluA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

rapA rluA yabQ yabP TSS_117 TSS_117 TSS_116 TSS_116 rluAp1 rluAp1

Gene      
Name: rluA    Texpresso search in the literature
Synonym(s): ECK0059, EG12609, b0058, yabO
Genome position(nucleotides): 59687 <-- 60346 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.94
External database links:  
ASAP:
ABE-0000196
CGSC:
36966
ECHOBASE:
EB2493
OU-MICROARRAY:
b0058
PortEco:
rluA
STRING:
511145.b0058
COLOMBOS: rluA


Product      
Name: 23S rRNA pseudouridine746 and tRNA pseudouridine32 synthase
Synonym(s): 23S rRNA and tRNA pseudouridine synthase, RluA, YabO, ribosomal large subunit pseudouridine synthase A
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 24.861
Isoelectric point: 8.244
Motif(s):
 
Type Positions Sequence
128 -> 128 C
2 -> 219 GMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKDSVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWGHPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGRSHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPADF
22 -> 169 HIMVVNKPSGLLSVPGRLEEHKDSVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWGHPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGRSHQLRVHML
28 -> 28 K
64 -> 64 D

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.2 - RNA related --> 2.2.3 - RNA modification
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0106029 - tRNA pseudouridine synthase activity
GO:0120159 - rRNA pseudouridine synthase activity
GO:0016853 - isomerase activity
GO:0003723 - RNA binding
GO:0009982 - pseudouridine synthase activity
biological_process GO:0009451 - RNA modification
GO:0001522 - pseudouridine synthesis
GO:0031118 - rRNA pseudouridine synthesis
GO:0031119 - tRNA pseudouridine synthesis
GO:0008033 - tRNA processing
GO:0006364 - rRNA processing
GO:0000455 - enzyme-directed rRNA pseudouridine synthesis
Note(s): Note(s): ...[more].
External database links:  
DIP:
DIP-47988N
ECOCYC:
EG12609-MONOMER
ECOLIWIKI:
b0058
INTERPRO:
IPR006145
INTERPRO:
IPR020103
INTERPRO:
IPR006225
INTERPRO:
IPR006224
PDB:
2I82
PFAM:
PF00849
PRIDE:
P0AA37
PRODB:
PRO_000023776
PROSITE:
PS01129
REFSEQ:
NP_414600
SMR:
P0AA37
SWISSMODEL:
P0AA37
UNIPROT:
P0AA37


Operon      
Name: rapA-rluA         
Operon arrangement:
Transcription unit        Promoter
rapA-rluA
hepA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter rluAp1 60450 reverse Similarity to the consensus
Read more >
[ICWHO] [1]
  promoter TSS_116 62712 reverse nd [RS-EPT-CBR] [2]
  promoter TSS_117 62992 reverse nd [RS-EPT-CBR] [2]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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