RegulonDB

Gene
Name:	yfaL
Synonym(s):	ECK2225, EG12850, b2233, yfaF, yfaJ, yfaK
Genome position(nucleotides):	2340417 <-- 2344169 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.37
External database links:

ASAP:	ABE-0007377
ECHOBASE:	EB2695
OU-MICROARRAY:	b2233
PortEco:	yfaL
STRING:	511145.b2233
COLOMBOS:	yfaL

Product

Name:

putative autotransporter adhesin YfaL

Synonym(s):

YfaF, YfaJ, YfaK, YfaL

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

periplasmic space,outer membrane,extracellular space

Molecular weight:

131.153

Isoelectric point:

3.77

Motif(s):

Type	Positions	Sequence
	1 -> 28	MRIIFLRKEYLSLLPSMIASLFSANGVA
	28 -> 30	AAV
	29 -> 1250	AVTDSCQGYDVKASCQASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAMFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIVNNTAFTNNTAEGYGGAIYTNSVTAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITSDVDTRSDAAGHGRDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYAWFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW
	853 -> 853	V
	933 -> 934	DP

Type	Positions	Sequence
	1 -> 28	MRIIFLRKEYLSLLPSMIASLFSANGVA
	28 -> 30	AAV
	29 -> 1250	AVTDSCQGYDVKASCQASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAMFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIVNNTAFTNNTAEGYGGAIYTNSVTAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITSDVDTRSDAAGHGRDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYAWFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW
	853 -> 853	V
	933 -> 934	DP
	40 -> 40	K
	186 -> 208	DVIFDNNQAYTSTSYSDGDGGAI
	919 -> 920	TP
	941 -> 942	TP
	980 -> 1250	AGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYAWFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW
	989 -> 1228	LRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYAWFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEIKVGVTGNISQRVSLRGSV
	127 -> 144	DVIFSGNVAGGYGGAIYS
	230 -> 245	AFTNNTAEGYGGAIYT
	937 -> 938	DP
	948 -> 994	PAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGG
	697 -> 1250	ILPYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYAWFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW
	920 -> 952	PPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQP
	921 -> 922	PS
	927 -> 928	DP
	929 -> 930	TP
	328 -> 355	ITKTGSGDLVLNADNNDFTGEMQIENGE
	478 -> 478	A
	947 -> 948	TP
	65 -> 66	LV
	559 -> 580	GQVSLANNNSYLGTTQIASGTL
	663 -> 690	LTKTGAGTLELTASGTTQSAVRVEEGTL
	939 -> 940	DP
	431 -> 431	S
	773 -> 773	E
	935 -> 936	TP
	433 -> 434	AG
	923 -> 924	PP
	925 -> 926	DP
	931 -> 932	DP
	943 -> 944	DP
	945 -> 946	EP

Classification:

Multifun Terms (GenProtEC)
Gene Ontology Terms (GO)
	cellular_component	GO:0009279 - cell outer membrane GO:0019867 - outer membrane GO:0016020 - membrane GO:0042597 - periplasmic space GO:0005576 - extracellular region GO:0009986 - cell surface
	molecular_function	GO:0015475 - adhesin autotransporter activity GO:0008233 - peptidase activity GO:0016787 - hydrolase activity GO:0008236 - serine-type peptidase activity
	biological_process	GO:0006508 - proteolysis GO:0009411 - response to UV GO:0043709 - cell adhesion involved in single-species biofilm formation

Note(s):

Note(s): ...[more].

Evidence:

[IMP] Inferred from mutant phenotype

Reference(s):

[1] Roux A., et al., 2005
[2] Wells TJ., et al., 2010

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

PFAM:

PFAM:

PFAM:

PRIDE:

PROSITE:

REFSEQ:

SMART:

SMR:

UNIPROT:

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	yfaLp8	2344207	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 5.253. P-value: 3.802e-6 Read more >	[ICWHO]	[3]
promoter	yfaLp1	2344218	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: -2.81. P-value: 3.501e-5 Read more >	[ICWHO]	[3]
promoter	yfaLp3	2344265	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 2.376. P-value: 8.272e-5 Read more >	[ICWHO]	[3]
promoter	yfaLp7	2344284	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.758. P-value: 3.607e-5 Read more >	[ICWHO]	[3]

Reference(s)
[1] Roux A., Beloin C., Ghigo JM., 2005, Combined inactivation and expression strategy to study gene function under physiological conditions: application to identification of new Escherichia coli adhesins., J Bacteriol 187(3):1001-13
[2] Wells TJ., Totsika M., Schembri MA., 2010, Autotransporters of Escherichia coli: a sequence-based characterization., Microbiology 156(Pt 8):2459-69
[3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78