RegulonDB

Gene
Name:	alaW
Synonym(s):	ECK2391, EG30011, alaWalpha, b2397
Genome position(nucleotides):	2518156 <-- 2518231 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	60.53
External database links:

ASAP:	ABE-0007904
CGSC:	32851
ECHOBASE:	EB4174
OU-MICROARRAY:	b2397
PortEco:	alaW
COLOMBOS:	alaW

Product

Name:

tRNA-Ala(GGC)

Synonym(s):

tRNA^alaW

Type:

tRNAs

Cellular location:

cytosol

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		2 - information transfer --> 2.2 - RNA related --> 2.2.5 - tRNA
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0030533 - triplet codon-amino acid adaptor activity

Note(s):

Note(s): ...[more].

Evidence:

[TAS] Traceable author statement

Reference(s):

[1] Siekevitz P., et al., 1981

External database links:

ECOCYC:

alaW-tRNA

ECOLIWIKI:

b2397

Operon

Name:

alaWX

Operon arrangement:

Transcription unit	Promoter
alaWX	alaWp

Transcriptional Regulation
	Display Regulation
Activated by:	Fis

RNA cis-regulatory element

Attenuation:	Translational

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	alaXp5	2518259	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 6.416. P-value: 2.129e-6 Read more >	[ICWHO]	[2]
promoter	alaXp2	2518279	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.136. P-value: 4.406e-5 Read more >	[ICWHO]	[2]
promoter	alaXp4	2518306	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.19. P-value: 4.632e-5 Read more >	[ICWHO]	[2]
promoter	yfeCp5	2518334	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.528. P-value: 4.968e-5 Read more >	[ICWHO]	[2]
promoter	yfeCp4	2518375	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.166. P-value: 2.953e-5 Read more >	[ICWHO]	[2]
promoter	yfeCp3	2518384	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: 3.084. P-value: 8.872e-5 Read more >	[ICWHO]	[2]
promoter	TSS_2681 (cluster)	2518419	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 2518419 to 2518420, where the highest frequency is at position 2516442. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Siekevitz P., Zamecnik PC., 1981, Ribosomes and protein synthesis., J Cell Biol 91(3 Pt 2):53s-65s
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.