RegulonDB

Gene
Name:	asnU
Synonym(s):	ECK1981, EG30021, b1986
Genome position(nucleotides):	2059851 --> 2059926 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.63
External database links:

ASAP:	ABE-0006588
CGSC:	17746
ECHOBASE:	EB4184
OU-MICROARRAY:	b1986
PortEco:	asnU
COLOMBOS:	asnU

Product

Name:

tRNA-Asn(GUU)

Synonym(s):

tRNA^asnU

Type:

tRNAs

Cellular location:

cytosol

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		2 - information transfer --> 2.2 - RNA related --> 2.2.5 - tRNA
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0030533 - triplet codon-amino acid adaptor activity

Note(s):

Note(s): ...[more].

Evidence:

[TAS] Traceable author statement

Reference(s):

[1] Kimura-Harada F., et al., 1972
[2] Siekevitz P., et al., 1981

External database links:

ECOCYC:

ECOLIWIKI:

Operon

Name:

asnU

Operon arrangement:

Transcription unit	Promoter
asnU	asnUp

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_2296	2059688	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_2297	2059690	reverse	nd	[RS-EPT-CBR]	[3]
promoter	yeeOp7	2059722	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.26. P-value: 2.866e-5 Read more >	[ICWHO]	[4]
promoter	yeeOp1	2059828	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: -1.53. P-value: 6.928e-6 Read more >	[ICWHO]	[4]
promoter	TSS_2298	2059842	forward	nd	[RS-EPT-CBR]	[3]
promoter	TSS_2299	2059851	forward	nd	[RS-EPT-CBR]	[3]
promoter	yeeOp6	2059875	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.505. P-value: 6.773e-5 Read more >	[ICWHO]	[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Kimura-Harada F., Harada F., Nishimura S., 1972, The presence of N-[9-(c-D-ribofuranosyl)purin-6-ylcarbamoyl] threonine in isoleucine, threonine and asparagine tRNAs from Escherichia coli., FEBS Lett 21(1):71-74
[2] Siekevitz P., Zamecnik PC., 1981, Ribosomes and protein synthesis., J Cell Biol 91(3 Pt 2):53s-65s
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.
[4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78