RegulonDB

Gene
Name:	metY
Synonym(s):	ECK3160, EG30061, b3171
Genome position(nucleotides):	3318213 <-- 3318289 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	64.94
External database links:

ASAP:	ABE-0010420
CGSC:	504
ECHOBASE:	EB4224
OU-MICROARRAY:	b3171
PortEco:	metY
COLOMBOS:	metY

Product

Name:

tRNA-initiator Met(CAU)

Synonym(s):

tRNA^fMet2, tRNA^metY

Type:

tRNAs

Cellular location:

cytosol

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		2 - information transfer --> 2.2 - RNA related --> 2.2.5 - tRNA
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0030533 - triplet codon-amino acid adaptor activity

Note(s):

Note(s): ...[more].

Evidence:

[TAS] Traceable author statement

Reference(s):

[1] Shetty S., et al., 2016
[2] Siekevitz P., et al., 1981

External database links:

ECOCYC:

ECOLIWIKI:

Operon

Name:

Operon arrangement:

Transcription unit	Promoter
pnp	pnpp
rpsO	rpsOp
rpsO-pnp	rpsOp
infB	infBp
nusA-infB	nusAp
rimP-nusA-infB	rimPp
metY	metYp1
metY-yhbC-nusA-infB	metYp1
metY-yhbC-nusA-infB-rbfA-truB-rpsO-pnp	metYp1
metY	metYp2
metY-yhbC-nusA-infB-rbfA-truB-rpsO-pnp	metYp2

Transcriptional Regulation
	Display Regulation
Activated by:	Fis
Repressed by:	CRP, ArgR

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	TSS_3467 (cluster)	3317609	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 3317608 to 3317609, where the highest frequency is at position 3315631. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]
promoter	TSS_3468	3317627	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_3469 (cluster)	3317768	reverse	For this promoter, there is a cluster of 2 transcription start sites,in the interval 3317767 to 3317768, where the highest frequency is at position 3315790. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]
promoter	TSS_3470	3317880	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_3471	3317926	reverse	nd	[RS-EPT-CBR]	[3]
promoter	TSS_3473	3318996	forward	nd	[RS-EPT-CBR]	[3]
promoter	TSS_3474 (cluster)	3318998	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 3318998 to 3318999, where the highest frequency is at position 3317020. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]
promoter	TSS_3475	3319007	forward	nd	[RS-EPT-CBR]	[3]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Shetty S., Varshney U., 2016, An evolutionarily conserved element in initiator tRNAs prompts ultimate steps in ribosome maturation., Proc Natl Acad Sci U S A 113(41):E6126-E6134
[2] Siekevitz P., Zamecnik PC., 1981, Ribosomes and protein synthesis., J Cell Biol 91(3 Pt 2):53s-65s
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.