RegulonDB

Gene
Name:	gadA
Synonym(s):	b3517, gadS
Genome position(nucleotides):	3664203 <-- 3665603 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.96
External database links:

ECOCYC:	EG50009
GENBANK:	1789934
GENBANK_RefSeq:	16131389
OU-MICROARRAY:	b3517
M3D:	gadA
COLOMBOS:	gadA
PortEco:	b3517

Product

Name:

glutamate decarboxylase A subunit

Synonym(s):

GadA, GadS

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol,membrane

Molecular weight:

52.685

Isoelectric point:

5.04

Motif(s):

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		5 - cell processes --> 5.5 - adaptations --> 5.5.4 - pH
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol GO:0016020 - membrane
	molecular_function	GO:0003824 - catalytic activity GO:0016829 - lyase activity GO:0016831 - carboxy-lyase activity GO:0004351 - glutamate decarboxylase activity GO:0030170 - pyridoxal phosphate binding
	biological_process	GO:0019752 - carboxylic acid metabolic process GO:0006536 - glutamate metabolic process GO:0051454 - intracellular pH elevation

Note(s):

Note(s): ...[more].

Reference(s):

External database links:

ECOCYC:

EXPASY:

GENPROTEC:

PDB:

Operon

Name:

gadAXW

Operon arrangement:

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_3787	3665178		reverse	nd	[RS-EPT-CBR]	[4]
	promoter	TSS_3788	3666802		reverse	nd	[RS-EPT-CBR]	[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] De Biase D., Tramonti A., Bossa F., Visca P., 1999, The response to stationary-phase stress conditions in Escherichia coli: role and regulation of the glutamic acid decarboxylase system., Mol Microbiol. 32(6):1198-211
[2] Lin J., Lee IS., Frey J., Slonczewski JL., Foster JW., 1995, Comparative analysis of extreme acid survival in Salmonella typhimurium, Shigella flexneri, and Escherichia coli., J Bacteriol. 177(14):4097-104
[3] Smith DK., Kassam T., Singh B., Elliott JF., 1992, Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci., J Bacteriol. 174(18):5820-6
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Mu¿¿iz-Rascado L, Garc¿¿a-Sotelo JS, Weiss V, Solano-Lira H, Mart¿¿nez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hern¿¿ndez S, Alquicira-Hern¿¿ndez K, L¿¿pez-Fuentes A, Porr¿¿n-Sotelo L, Huerta AM, Bonavides-Mart¿¿nez C, Balderas-Mart¿¿nez YI, Pannier L, Olvera M, Labastida A, Jim¿¿nez-Jacinto V, Vega-Alvarado L, Del Moral-Ch¿¿vez V, Hern¿¿ndez-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.