RegulonDB RegulonDB 9.1: Gene Form
   

gadA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

gadA yhjA gadX Fis TorR GadX GadW Fis GadX GadW Fis GadX GadW GadE-RcsB CRP GadX GadW GadX RcsB Fis GadX FNR TorR RutR FNR GadY terminator TSS_4130 TSS_4130 gadAp gadAp TSS_4129 TSS_4129 gadXp gadXp

Gene      
Name: gadA    Texpresso search in the literature
Synonym(s): b3517, gadS
Genome position(nucleotides): 3664203 <-- 3665603 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.96
External database links:  
ECOCYC:
 EG50009
GENBANK:
 1789934
GENBANK_RefSeq:
 16131389
OU-MICROARRAY:
 b3517
M3D: gadA
COLOMBOS: gadA
PortEco: b3517


Product      
Name: glutamate decarboxylase A subunit
Synonym(s): GadA, GadS
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,membrane
Molecular weight: 52.685
Isoelectric point: 5.04
Motif(s):
 
Type Positions Sequence
73 -> 73 H
295 -> 295 L
165 -> 165 C
208 -> 208 T
355 -> 355 D

 
Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.5 - adaptations --> 5.5.4 - pH
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0016020 - membrane
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016829 - lyase activity
GO:0016831 - carboxy-lyase activity
GO:0004351 - glutamate decarboxylase activity
GO:0030170 - pyridoxal phosphate binding
biological_process GO:0019752 - carboxylic acid metabolic process
GO:0006536 - glutamate metabolic process
GO:0051454 - intracellular pH elevation
Note(s): Note(s): ...[more].
Reference(s): [1] De Biase D., et al., 1999
[2] Lin J., et al., 1995
[3] Smith DK., et al., 1992
External database links:  
ECOCYC:
 GLUTDECARBOXA-MONOMER
EXPASY:
 EC-4.1.1.15
GENPROTEC:
 b3517
PDB:
 1XEY


Operon      
Name: gadAXW         
Operon arrangement:
Transcription unit        Promoter
gadW
gadW
gadX
gadXW
gadAX


Transcriptional Regulation      
Display Regulation             
Note: The TF binding sites with unknown position are not displayed in the graphic.
Activated by: GadX, GadE-RcsB, GadW, ArcA, AdiY
Repressed by: CRP, FNR, H-NS, GadW, RcsB, TorR, Fis


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4129 3665178 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_4130 3666802 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] De Biase D., Tramonti A., Bossa F., Visca P., 1999, The response to stationary-phase stress conditions in Escherichia coli: role and regulation of the glutamic acid decarboxylase system., Mol Microbiol. 32(6):1198-211

 [2] Lin J., Lee IS., Frey J., Slonczewski JL., Foster JW., 1995, Comparative analysis of extreme acid survival in Salmonella typhimurium, Shigella flexneri, and Escherichia coli., J Bacteriol. 177(14):4097-104

 [3] Smith DK., Kassam T., Singh B., Elliott JF., 1992, Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci., J Bacteriol. 174(18):5820-6

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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