RegulonDB RegulonDB 11.2: Gene Form
   

gadA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

gadA ccp gadX Fis TorR GadX GadW Fis GadX GadW Fis GadX GadW GadE-RcsB CRP GadX GadW GadX RcsB Fis GadX FNR TorR RutR FNR ppGpp terminator TSS_4130 TSS_4130 gadAp2 gadAp2 gadAp gadAp TSS_4129 TSS_4129 gadXp gadXp

Gene      
Name: gadA    Texpresso search in the literature
Synonym(s): ECK3502, EG50009, b3517, gadS
Genome position(nucleotides): 3666180 <-- 3667580
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.96
External database links:  
ASAP:
 ABE-0011490
CGSC:
 32191
ECHOBASE:
 EB4302
ECOLIHUB:
 gadA
OU-MICROARRAY:
 b3517
STRING:
 511145.b3517
COLOMBOS: gadA


Product      
Name: glutamate decarboxylase A
Synonym(s): GadA, GadS
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol,membrane
Molecular weight: 52.685
Isoelectric point: 5.041
Motif(s):
 
Type Positions Sequence Comment
33 -> 383 PLHEMRDDVAFQIINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLK
64 -> 64 C UniProt: In Ref. 2; AA sequence..
73 -> 73 H UniProt: In Ref. 2; AA sequence..
126 -> 127 SS UniProt: Pyridoxal phosphate binding; Sequence Annotation Type: region of interest.
153 -> 153 D UniProt: In Ref. 2; AA sequence..

 
Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.5 - adaptations --> 5.5.4 - pH
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
GO:0016020 - membrane
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016829 - lyase activity
GO:0016830 - carbon-carbon lyase activity
GO:0016831 - carboxy-lyase activity
GO:0004351 - glutamate decarboxylase activity
GO:0030170 - pyridoxal phosphate binding
biological_process GO:0006538 - glutamate catabolic process
GO:0019752 - carboxylic acid metabolic process
GO:0006536 - glutamate metabolic process
GO:0051454 - intracellular pH elevation
Note(s): Note(s): ...[more].
Reference(s): [1] De Biase D., et al., 1999
[2] Kanda T., et al., 2020
[3] Lin J., et al., 1995
[4] Smith DK., et al., 1992
External database links:  
ALPHAFOLD:
 P69908
DIP:
 DIP-36201N
ECOCYC:
 GLUTDECARBOXA-MONOMER
ECOLIWIKI:
 b3517
INTERPRO:
 IPR010107
INTERPRO:
 IPR015421
INTERPRO:
 IPR002129
INTERPRO:
 IPR015424
INTERPRO:
 IPR021115
MINT:
 P69908
MODBASE:
 P69908
PANTHER:
 PTHR43321
PDB:
 1XEY
PFAM:
 PF00282
PRIDE:
 P69908
PRODB:
 PRO_000022741
PROSITE:
 PS00392
REFSEQ:
 NP_417974
SMR:
 P69908
UNIPROT:
 P69908


Operon      
Name: gadAXW         
Operon arrangement:
Transcription unit        Promoter
gadW
gadW
gadX
gadXW
gadAX
gadAX


Transcriptional Regulation      
Display Regulation             
Activated by: GadX, GadE-RcsB, ArcA, GadW, AdiY
Repressed by: CRP, H-NS, FNR, GadW, RcsB, TorR, Fis


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4129 3667155 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_4130 3668779 reverse nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] De Biase D., Tramonti A., Bossa F., Visca P., 1999, The response to stationary-phase stress conditions in Escherichia coli: role and regulation of the glutamic acid decarboxylase system., Mol Microbiol 32(6):1198-211

 [2] Kanda T., Abiko G., Kanesaki Y., Yoshikawa H., Iwai N., Wachi M., 2020, RNase E-dependent degradation of tnaA mRNA encoding tryptophanase is prerequisite for the induction of acid resistance in Escherichia coli., Sci Rep 10(1):7128

 [3] Lin J., Lee IS., Frey J., Slonczewski JL., Foster JW., 1995, Comparative analysis of extreme acid survival in Salmonella typhimurium, Shigella flexneri, and Escherichia coli., J Bacteriol 177(14):4097-104

 [4] Smith DK., Kassam T., Singh B., Elliott JF., 1992, Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci., J Bacteriol 174(18):5820-6

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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