RegulonDB RegulonDB 10.6.3: Gene Form
   

gadA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

gadA ccp gadX Fis TorR GadX GadW Fis GadX GadW Fis GadX GadW GadE CRP GadX GadW GadX RcsB Fis GadX FNR TorR RutR FNR GadY ppGpp terminator TSS_4130 TSS_4130 gadAp gadAp TSS_4129 TSS_4129 gadXp gadXp

Gene      
Name: gadA    Texpresso search in the literature
Synonym(s): ECK3502, EG50009, b3517, gadS
Genome position(nucleotides): 3666180 <-- 3667580 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.96
External database links:  
ASAP:
ABE-0011490
CGSC:
32191
ECHOBASE:
EB4302
ECOCYC:
EG50009
ECOLIHUB:
gadA
OU-MICROARRAY:
b3517
REGULONDB:
b3517
STRING:
511145.b3517
M3D: gadA
COLOMBOS: gadA
PortEco: b3517


Product      
Name: glutamate decarboxylase A
Synonym(s): GadA, GadS
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 52.685
Isoelectric point: 5.041
Motif(s):
 
Type Positions Sequence
73 -> 73 H
295 -> 295 L
165 -> 165 C
208 -> 208 T
355 -> 355 D

 

Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.5 - adaptations --> 5.5.4 - pH
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0003824 - catalytic activity
GO:0005515 - protein binding
GO:0016829 - lyase activity
GO:0016831 - carboxy-lyase activity
GO:0004351 - glutamate decarboxylase activity
GO:0030170 - pyridoxal phosphate binding
biological_process GO:0006538 - glutamate catabolic process
GO:0019752 - carboxylic acid metabolic process
GO:0006536 - glutamate metabolic process
GO:0051454 - intracellular pH elevation
Note(s): Note(s): ...[more].
Reference(s): [1] De Biase D., et al., 1999
[2] Lin J., et al., 1995
[3] Smith DK., et al., 1992
External database links:  
DIP:
DIP-36201N
ECOCYC:
GLUTDECARBOXA-MONOMER
ECOLIWIKI:
b3517
INTERPRO:
IPR015421
INTERPRO:
IPR021115
INTERPRO:
IPR015424
INTERPRO:
IPR010107
INTERPRO:
IPR002129
MINT:
P69908
MODBASE:
P69908
PANTHER:
PTHR43321
PDB:
1XEY
PFAM:
PF00282
PRIDE:
P69908
PRODB:
PRO_000022741
PROSITE:
PS00392
PROTEINMODELPORTAL:
P69908
REFSEQ:
NP_417974
SMR:
P69908
UNIPROT:
P69908


Operon      
Name: gadAXW         
Operon arrangement:
Transcription unit        Promoter
gadW
gadW
gadX
gadXW
gadAX


Transcriptional Regulation      
Display Regulation             
Activated by: GadX, GadW, ArcA, AdiY, GadE
Repressed by: CRP, H-NS, FNR, GadW, TorR, RcsB, Fis


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_4129 3667155 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_4130 3668779 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] De Biase D., Tramonti A., Bossa F., Visca P., 1999, The response to stationary-phase stress conditions in Escherichia coli: role and regulation of the glutamic acid decarboxylase system., Mol Microbiol 32(6):1198-211

 [2] Lin J., Lee IS., Frey J., Slonczewski JL., Foster JW., 1995, Comparative analysis of extreme acid survival in Salmonella typhimurium, Shigella flexneri, and Escherichia coli., J Bacteriol 177(14):4097-104

 [3] Smith DK., Kassam T., Singh B., Elliott JF., 1992, Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci., J Bacteriol 174(18):5820-6

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Mu¿¿iz-Rascado L, Garc¿¿a-Sotelo JS, Weiss V, Solano-Lira H, Mart¿¿nez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hern¿¿ndez S, Alquicira-Hern¿¿ndez K, L¿¿pez-Fuentes A, Porr¿¿n-Sotelo L, Huerta AM, Bonavides-Mart¿¿nez C, Balderas-Mart¿¿nez YI, Pannier L, Olvera M, Labastida A, Jim¿¿nez-Jacinto V, Vega-Alvarado L, Del Moral-Ch¿¿vez V, Hern¿¿ndez-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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