RegulonDB

Gene
Name:	ecnB
Synonym(s):	ECK4143, G0-9562, b4411, yjeU
Genome position(nucleotides):	4376553 --> 4376699 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	48.3
External database links:

ASAP:	ABE-0047211
ECHOBASE:	EB4091
ECOLIHUB:	ecnB
STRING:	511145.b4411
COLOMBOS:	ecnB

Product

Name:

bacteriolytic entericidin B lipoprotein

Synonym(s):

EcnB, YjeU

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane,outer membrane

Molecular weight:

4.81

Isoelectric point:

8.536

Motif(s):

Type	Positions	Sequence
	1 -> 21	MVKKTIAAIFSVLVLSTVLTA
	24 -> 43	TTRGVGEDISDGGNAISGAA

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		5 - cell processes --> 5.5 - adaptations --> 5.5.1 - osmotic pressure
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0036405 - anchored component of cell outer membrane
	biological_process	GO:0009636 - response to toxic substance

Note(s):

Note(s): ...[more].

Evidence:

[AUPEINH] Assay of unpurified protein expressed in its native host

Reference(s):

[1] Alix E., et al., 2009
[2] Bishop RE., et al., 1998
[3] Gonnet P., et al., 2004
[4] Rao S., et al., 2020
[5] Schellhorn HE., et al., 1998

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

PROSITE:

REFSEQ:

UNIPROT:

Operon

Name:

ecnAB

Operon arrangement:

Transcription unit	Promoter
ecnB	ecnBp
ecnAB

Transcriptional Regulation
	Display Regulation
Repressed by:	OmpR

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_4966	4376799		forward	nd	[RS-EPT-CBR]	[6]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Alix E., Blanc-Potard AB., 2009, Hydrophobic peptides: novel regulators within bacterial membrane., Mol Microbiol 72(1):5-11
[2] Bishop RE., Leskiw BK., Hodges RS., Kay CM., Weiner JH., 1998, The entericidin locus of Escherichia coli and its implications for programmed bacterial cell death., J Mol Biol 280(4):583-96
[3] Gonnet P., Rudd KE., Lisacek F., 2004, Fine-tuning the prediction of sequences cleaved by signal peptidase II: a curated set of proven and predicted lipoproteins of Escherichia coli K-12., Proteomics 4(6):1597-613
[4] Rao S., Bates GT., Matthews CR., Newport TD., Vickery ON., Stansfeld PJ., 2020, Characterizing Membrane Association and Periplasmic Transfer of Bacterial Lipoproteins through Molecular Dynamics Simulations., Structure 28(4):475-487.e3
[5] Schellhorn HE., Audia JP., Wei LI., Chang L., 1998, Identification of conserved, RpoS-dependent stationary-phase genes of Escherichia coli., J Bacteriol 180(23):6283-91
[6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.