RegulonDB RegulonDB 10.8: Gene Form
   

tisB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

istR tisA tisB istR-1 ysdE LexA IstR-2 IstR-1 IstR-2 IstR-1 terminator anti-anti-terminator anti-terminator anti-anti-terminator anti-terminator terminator TSS_4252 TSS_4252 tisBp tisBp istR-2p istR-2p istR-1p istR-1p

Gene      
Name: tisB    Texpresso search in the literature
Synonym(s): ECK4427, G0-9962, b4618, ysdB
Genome position(nucleotides): 3853553 --> 3853642 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 42.22
External database links:  
PortEco:
 tisB
STRING:
 511145.b4618
COLOMBOS: tisB


Product      
Name: membrane-depolarizing toxin TisB
Synonym(s): TisB, YsdB, toxic peptide TisB
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 3.222
Isoelectric point: 9.224
Motif(s):
 
Type Positions Sequence
3 -> 25 LVDIAILILKLIVAALQLLDAVL
1 -> 28 MNLVDIAILILKLIVAALQLLDAVLKYL

 
Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.8 - SOS response
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0005253 - anion channel activity
biological_process GO:0006974 - cellular response to DNA damage stimulus
GO:0009432 - SOS response
GO:0015698 - inorganic anion transport
GO:0022611 - dormancy process
Note(s): Note(s): ...[more].
Evidence: [IMP] Inferred from mutant phenotype
Reference(s): [1] Berghoff BA., et al., 2017
[2] Dorr T., et al., 2010
[3] Vogel J., et al., 2004
External database links:  
ECOCYC:
 MONOMER0-1922
ECOLIWIKI:
 b4618
INTERPRO:
 IPR025211
PFAM:
 PF13939
PRIDE:
 A5A627
REFSEQ:
 YP_001165331
UNIPROT:
 A5A627


Operon      
Name: tisB         
Operon arrangement:
Transcription unit        Promoter
tisB


Transcriptional Regulation      
Display Regulation             
Repressed by: LexA


Regulation by small RNA    
  Display Regulation
small RNA istR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  srna IstR-1 3853418 3853461 forward unknow. nd nd
  srna IstR-2 3853418 3853461 forward unknow. nd nd
  promoter TSS_4252 3853442 forward nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Berghoff BA., Hoekzema M., Aulbach L., Wagner EG., 2017, Two regulatory RNA elements affect TisB-dependent depolarization and persister formation., Mol Microbiol 103(6):1020-1033

 [2] Dorr T., Vulic M., Lewis K., 2010, Ciprofloxacin causes persister formation by inducing the TisB toxin in Escherichia coli., PLoS Biol 8(2):e1000317

 [3] Vogel J., Argaman L., Wagner EG., Altuvia S., 2004, The small RNA IstR inhibits synthesis of an SOS-induced toxic peptide., Curr Biol 14(24):2271-6

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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