RegulonDB RegulonDB 10.7: Gene Form
   

sapB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

sapA sapD sapB sapC ymjA TSS_1738 TSS_1738 TSS_1737 TSS_1737 TSS_1736 TSS_1736 TSS_1735 TSS_1735 sapFp5 sapFp5

Gene      
Name: sapB    Texpresso search in the literature
Synonym(s): ECK1288, G2001, b1293
Genome position(nucleotides): 1354505 <-- 1355470 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.45
External database links:  
ASAP:
ABE-0004350
ECHOBASE:
EB4156
OU-MICROARRAY:
b1293
PORTECO:
sapB
REGULONDB:
b1293
STRING:
511145.b1293
M3D: sapB
COLOMBOS: sapB
PortEco: b1293


Product      
Name: putrescine ABC exporter membrane subunit SapB
Synonym(s): SapB
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 36.038
Isoelectric point: 9.165
Motif(s):
 
Type Positions Sequence
281 -> 301 YAAISAGVMVCGSLVIIVNVI
92 -> 311 GIPVGMIAGITRHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFALIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAAATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAIRQQDYAAISAGVMVCGSLVIIVNVISDILGAMANP
113 -> 133 AIALLGFSIPVFWLALLLTLF
249 -> 269 FSTMLTLAMITEMVFSWPGLG
74 -> 302 FPATMELCILAFGFALIVGIPVGMIAGITRHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFALIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAAATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAIRQQDYAAISAGVMVCGSLVIIVNVIS

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.2 - degradation of macromolecules --> 1.2.3 - proteins/peptides/glycopeptides
  6 - cell structure --> 6.1 - membrane
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.m - ABC superfamily, membrane component
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0043190 - ATP-binding cassette (ABC) transporter complex
molecular_function GO:0015489 - putrescine transmembrane transporter activity
GO:0071916 - dipeptide transmembrane transporter activity
biological_process GO:0055085 - transmembrane transport
GO:0015847 - putrescine transport
GO:0035442 - dipeptide transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Jindal S., et al., 2019
External database links:  
ECOCYC:
SAPB-MONOMER
ECOLIWIKI:
b1293
INTERPRO:
IPR000515
INTERPRO:
IPR035906
PFAM:
PF00528
PRIDE:
P0AGH3
PRODB:
PRO_000023905
PROSITE:
PS50928
PROTEINMODELPORTAL:
P0AGH3
REFSEQ:
NP_415809
UNIPROT:
P0AGH3


Operon      
Name: sapABCDF         
Operon arrangement:
Transcription unit        Promoter
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter sapFp5 1352657 reverse Similarity to the consensus
Read more >
[ICWHO] [2]
  promoter TSS_1735 1353593 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_1736 1353743 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_1737 1355493 reverse nd [RS-EPT-CBR] [3]
  promoter TSS_1738 1356037 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Jindal S., Yang L., Day PJ., Kell DB., 2019, Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli., BMC Microbiol 19(1):195

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


RegulonDB