RegulonDB

Gene
Name:	flgA
Synonym(s):	ECK1057, G357, b1072, flaU
Genome position(nucleotides):	1130204 <-- 1130863 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	52.27
External database links:

ASAP:	ABE-0003637
CGSC:	756
ECHOBASE:	EB4160
OU-MICROARRAY:	b1072
PortEco:	flgA
STRING:	511145.b1072
COLOMBOS:	flgA

Product

Name:

flagellar basal body P-ring formation protein FlgA

Synonym(s):

FlaU, FlgA

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

periplasmic space

Molecular weight:

23.519

Isoelectric point:

10.804

Motif(s):

Type	Positions	Sequence
	1 -> 21	MLIIKRSVAIIAILFSPLSTA
	96 -> 215	NYVVAAMPIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAWRVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNI
	22 -> 219	SNLTSQLHNFFSAQLAGVSDEVRVSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAMPIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAWRVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILINL

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.12 - flagellum
		5 - cell processes --> 5.3 - motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)
		6 - cell structure --> 6.4 - flagella
Gene Ontology Terms (GO)
	cellular_component	GO:0030288 - outer membrane-bounded periplasmic space GO:0042597 - periplasmic space
	biological_process	GO:0071973 - bacterial-type flagellum-dependent cell motility GO:0044780 - bacterial-type flagellum assembly GO:0044781 - bacterial-type flagellum organization

Note(s):

Note(s): ...[more].

Evidence:

[IMP] Inferred from mutant phenotype

Reference(s):

[1] Juhas M., et al., 2015
[2] Ohnishi K., et al., 1987

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMART:

SMR:

UNIPROT:

Operon

Name:

flgAMN

Operon arrangement:

Transcription unit	Promoter
flgMN	flgMp
flgAMN	flgAp

Transcriptional Regulation
	Display Regulation
Activated by:	FlhDC

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	flgNp1	1129914	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: -3.3. P-value: 6.128e-5 Read more >	[ICWHO]	[3]
promoter	TSS_1342	1130164	reverse	nd	[RS-EPT-CBR]	[4]
promoter	TSS_1343	1130193	reverse	nd	[RS-EPT-CBR]	[4]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Juhas M., Ajioka JW., 2015, Identification and Validation of Novel Chromosomal Integration and Expression Loci in Escherichia coli Flagellar Region 1., PLoS One 10(3):e0123007
[2] Ohnishi K., Homma M., Kutsukake K., Iino T., 1987, Formation of flagella lacking outer rings by flaM, flaU, and flaY mutants of Escherichia coli., J Bacteriol 169(4):1485-8
[3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.