RegulonDB

Gene
Name:	yafO
Synonym(s):	ECK0234, G6117, b0233
Genome position(nucleotides):	252301 --> 252699 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	43.61
External database links:

ASAP:	ABE-0000795
ECHOBASE:	EB2946
OU-MICROARRAY:	b0233
PortEco:	yafO
STRING:	511145.b0233
COLOMBOS:	yafO

Product

Name:

ribosome-dependent mRNA interferase toxin YafO

Synonym(s):

YafO, ribosome-dependent mRNA interferase, toxin of the YafO-YafN toxin-antitoxin system

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

15.488

Isoelectric point:

7.732

Motif(s):

Type	Positions	Sequence
	27 -> 130	SYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRM

Classification:

Multifun Terms (GenProtEC)
		3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.1 - translation attenuation and efficiency
		5 - cell processes --> 5.6 - protection --> 5.6.3 - cell killing
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0004518 - nuclease activity GO:0004519 - endonuclease activity GO:0016787 - hydrolase activity GO:0003723 - RNA binding GO:0004521 - endoribonuclease activity GO:0043023 - ribosomal large subunit binding
	biological_process	GO:0006281 - DNA repair GO:0006974 - cellular response to DNA damage stimulus GO:0009432 - SOS response GO:0017148 - negative regulation of translation GO:0090305 - nucleic acid phosphodiester bond hydrolysis GO:0090502 - RNA phosphodiester bond hydrolysis, endonucleolytic

Note(s):

Note(s): ...[more].

Evidence:

[APPH] Assay of protein purified to homogeneity
[IMP] Inferred from mutant phenotype

Reference(s):

[1] Brown JM., et al., 2003
[2] Zhang Y., et al., 2009

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

PRODOM:

REFSEQ:

UNIPROT:

Operon

Name:

dinB-yafNOP

Operon arrangement:

Transcription unit	Promoter
dinB-yafNOP	dinBp
yafNOP	yafNp

Transcriptional Regulation
	Display Regulation
Repressed by:	LexA

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Brown JM., Shaw KJ., 2003, A novel family of Escherichia coli toxin-antitoxin gene pairs., J Bacteriol 185(22):6600-8
[2] Zhang Y., Yamaguchi Y., Inouye M., 2009, Characterization of YafO, an Escherichia coli Toxin., J Biol Chem 284(38):25522-31