RegulonDB

Gene
Name:	yaiO
Synonym(s):	ECK0355, G6210, b0358
Genome position(nucleotides):	380069 <-- 380842 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	50.13
External database links:

ASAP:	ABE-0001230
ECHOBASE:	EB3082
OU-MICROARRAY:	b0358
PortEco:	yaiO
STRING:	511145.b0358
COLOMBOS:	yaiO

Product

Name:

outer membrane protein YaiO

Synonym(s):

YaiO

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

outer membrane

Molecular weight:

29.04

Isoelectric point:

7.688

Motif(s):

Type	Positions	Sequence
	1 -> 19	MIKRTLLAAAIFSALPAYA
	20 -> 257	GLTSITAGYDFTDYSGDHGNRNLAYAELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTPVFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTHYDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQRIQPLTEQLNLGLTAGKVWYDTPTDDFNGLQLAARLTWKF

Classification:

Multifun Terms (GenProtEC)
Gene Ontology Terms (GO)
Gene Ontology Terms (GO)	cellular_component	GO:0009279 - cell outer membrane GO:0016020 - membrane

Note(s):

Note(s): ...[more].

Reference(s):

[1] Molina F., et al., 2015

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PRIDE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

yaiO

Operon arrangement:

Transcription unit	Promoter

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	yaiOp6	380850	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 6.38. P-value: 1.926e-6 Read more >	[ICWHO]	[2]
promoter	yaiOp4	380957	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.9. P-value: 8.610e-5 Read more >	[ICWHO]	[2]
promoter	yaiOp5	380973	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 4.429. P-value: 4.450e-5 Read more >	[ICWHO]	[2]
promoter	TSS_486 (cluster)	381322	forward	For this promoter, there is a cluster of 2 transcription start sites,in the interval 381322 to 381323, where the highest frequency is at position 380547. The most upstream position of this cluster is assigned as TSS position in the promoter. Read more >	[RS-EPT-CBR]	[3]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Molina F., Lopez-Acedo E., Tabla R., Roa I., Gomez A., Rebollo JE., 2015, Improved detection of Escherichia coli and coliform bacteria by multiplex PCR., BMC Biotechnol 15:48
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.