RegulonDB RegulonDB 10.7: Gene Form
   

yajO gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dxs yajO pgpA thiL TSS_538 (cluster) TSS_538 (cluster) TSS_537 TSS_537

Gene      
Name: yajO    Texpresso search in the literature
Synonym(s): ECK0413, G6236, b0419
Genome position(nucleotides): 437161 <-- 438135 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.03
External database links:  
ASAP:
ABE-0001457
ECHOBASE:
EB3377
OU-MICROARRAY:
b0419
PORTECO:
yajO
REGULONDB:
b0419
STRING:
511145.b0419
M3D: yajO
COLOMBOS: yajO
PortEco: b0419


Product      
Name: 1-deoxyxylulose-5-phosphate synthase YajO
Synonym(s): YajO
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 36.42
Isoelectric point: 4.965
Motif(s):
 
Type Positions Sequence
15 -> 312 RLCLGCMTFGEPDRGNHAWTLPEESSRPIIKRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSRAQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMHASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGRLTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSKPGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELE

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.5 - biosynthesis of building blocks --> 1.5.3 - cofactors, small molecule carriers
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016491 - oxidoreductase activity
biological_process GO:0006772 - thiamine metabolic process
GO:0055114 - oxidation-reduction process
GO:1902767 - isoprenoid biosynthetic process via mevalonate
Note(s): Note(s): ...[more].
Evidence: [IE] Inferred from experiment
[IFC] Inferred by functional complementation
Reference(s): [1] Kirby J., et al., 2015
[2] Lawhorn BG., et al., 2004
[3] Spindler EC., et al., 2013
External database links:  
DIP:
DIP-11291N
ECOCYC:
G6236-MONOMER
ECOLIWIKI:
b0419
INTERPRO:
IPR036812
INTERPRO:
IPR020471
INTERPRO:
IPR023210
MODBASE:
P77735
PANTHER:
PTHR11732
PFAM:
PF00248
PRIDE:
P77735
PRINTS:
PR00069
PROTEINMODELPORTAL:
P77735
REFSEQ:
NP_414953
SMR:
P77735
SWISSMODEL:
P77735
UNIPROT:
P77735


Operon      
Name: xseB-ispA-dxs-yajO         
Operon arrangement:
Transcription unit        Promoter
xseB-ispA-dxs-yajO
yajO


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_537 438284 reverse nd [RS-EPT-CBR] [4]
  promoter TSS_538 (cluster) 439919 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Kirby J., Nishimoto M., Chow RW., Baidoo EE., Wang G., Martin J., Schackwitz W., Chan R., Fortman JL., Keasling JD., 2015, Enhancing Terpene yield from sugars via novel routes to 1-deoxy-d-xylulose 5-phosphate., Appl Environ Microbiol 81(1):130-8

 [2] Lawhorn BG., Gerdes SY., Begley TP., 2004, A genetic screen for the identification of thiamin metabolic genes., J Biol Chem 279(42):43555-9

 [3] Spindler EC., Boyle NR., Hancock RE., Gill RT., 2013, Genome-wide identification of genes conferring energy related resistance to a synthetic antimicrobial peptide (Bac8c)., PLoS One 8(1):e55052

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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