RegulonDB RegulonDB 10.7: Gene Form
   

mscK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mscK acrR ybaM SoxS MarA anti-terminator terminator TSS_631 TSS_631 TSS_630 TSS_630 TSS_629 TSS_629 kefAp kefAp TSS_628 TSS_628 TSS_627 TSS_627 TSS_626 (cluster) TSS_626 (cluster)

Gene      
Name: mscK    Texpresso search in the literature
Synonym(s): ECK0459, G6255, aefA, b0465, kefA
Genome position(nucleotides): 486536 --> 489898 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
51.89
External database links:  
ASAP:
ABE-0001613
ECHOBASE:
EB3991
OU-MICROARRAY:
b0465
PORTECO:
kefA
REGULONDB:
b0465
STRING:
511145.b0465
M3D: mscK
COLOMBOS: mscK
PortEco: b0465


Product      
Name: potassium dependent, small conductance mechanosensitive channel
Synonym(s): AefA, KefA, MscK
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 127.215
Isoelectric point: 8.2
Motif(s):
 
Type Positions Sequence
635 -> 655 LALLPIHFWSVVAELSPLHLM
797 -> 817 LMFALFGVMFWAIWSDLITVF
500 -> 520 WPAVFIAFLAGLPLLLIAGLI
887 -> 907 TILNYIIIAVGAMTVFGSLGV
922 -> 942 GLGFGLQEIFGNFVSGLIILF

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.1 - Channel-type Transporters --> 4.1.A - alpha-type channels
  5 - cell processes --> 5.5 - adaptations --> 5.5.6 - other (mechanical, nutritional, oxidative stress)
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0005623 - cell
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0008381 - mechanosensitive ion channel activity
biological_process GO:0006811 - ion transport
GO:0006813 - potassium ion transport
GO:0009992 - cellular water homeostasis
GO:0055085 - transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Akitake B., et al., 2005
[2] Cui C., et al., 1996
[3] Cui C., et al., 1995
[4] Li C., et al., 2007
External database links:  
DIP:
DIP-10070N
ECOCYC:
G6255-MONOMER
ECOLIWIKI:
b0465
INTERPRO:
IPR011014
INTERPRO:
IPR011066
INTERPRO:
IPR023298
INTERPRO:
IPR023408
INTERPRO:
IPR024393
INTERPRO:
IPR025692
INTERPRO:
IPR006686
INTERPRO:
IPR006685
INTERPRO:
IPR010920
MODBASE:
P77338
PFAM:
PF00924
PFAM:
PF12795
PFAM:
PF12794
PRIDE:
P77338
PRODB:
PRO_000023060
PROSITE:
PS01246
PROTEINMODELPORTAL:
P77338
REFSEQ:
NP_414998
SMR:
P77338
UNIPROT:
P77338


Operon      
Name: mscK         
Operon arrangement:
Transcription unit        Promoter
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_626 (cluster) 485980 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [5]
  promoter TSS_627 486041 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_628 486086 reverse nd [RS-EPT-CBR] [5]
  promoter TSS_629 487911 forward nd [RS-EPT-CBR] [5]
  promoter TSS_630 488317 forward nd [RS-EPT-CBR] [5]
  promoter TSS_631 489636 forward nd [RS-EPT-CBR] [5]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Akitake B., Anishkin A., Sukharev S., 2005, The "dashpot" mechanism of stretch-dependent gating in MscS., J Gen Physiol 125(2):143-54

 [2] Cui C., Adler J., 1996, Effect of mutation of potassium-efflux system, KefA, on mechanosensitive channels in the cytoplasmic membrane of Escherichia coli., J Membr Biol 150(2):143-52

 [3] Cui C., Smith DO., Adler J., 1995, Characterization of mechanosensitive channels in Escherichia coli cytoplasmic membrane by whole-cell patch clamp recording., J Membr Biol 144(1):31-42

 [4] Li C., Edwards MD., Jeong H., Roth J., Booth IR., 2007, Identification of mutations that alter the gating of the Escherichia coli mechanosensitive channel protein, MscK., Mol Microbiol 64(2):560-74

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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