RegulonDB

Gene
Name:	chiP
Synonym(s):	ECK0669, G6370, b0681, ybfM
Genome position(nucleotides):	708334 --> 709740 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.24
External database links:

ASAP:	ABE-0002323
ECHOBASE:	EB3423
OU-MICROARRAY:	b0681
PortEco:	chiP
STRING:	511145.b0681
COLOMBOS:	chiP

Product

Name:

chitobiose outer membrane channel

Synonym(s):

ChiP, YbfM

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

outer membrane

Molecular weight:

52.78

Isoelectric point:

4.622

Motif(s):

Type	Positions	Sequence
	1 -> 32	MRTFSGKRSTLALAIAGVTAMSGFMAMPEARA
	33 -> 468	EGFIDDSTLTGGIYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAENGDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLLAPHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKVDYLHSFGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRDKVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYASSNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQSNPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNIFQDERDVKFMVIAPFTIF

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		4 - transport --> 4.1 - Channel-type Transporters --> 4.1.B - Beta barrel porins (The Outer Membrane Porin (OMP) Functional Superfamily)
Gene Ontology Terms (GO)
	cellular_component	GO:0009279 - cell outer membrane GO:0016020 - membrane GO:0016021 - integral component of membrane GO:0046930 - pore complex
	molecular_function	GO:0015267 - channel activity GO:0015288 - porin activity
	biological_process	GO:0006811 - ion transport GO:0008643 - carbohydrate transport GO:0015774 - polysaccharide transport GO:0055085 - transmembrane transport GO:0015772 - oligosaccharide transport GO:0052778 - diacetylchitobiose metabolic process

Note(s):

Note(s): ...[more].

Reference(s):

[1] Soysa HSM., et al., 2017
[2] Soysa HSM., et al., 2018
[3] Yang C., et al., 2006

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

chiPQ

Operon arrangement:

Transcription unit	Promoter
chiP-ybfN	chiPp

Transcriptional Regulation
	Display Regulation
Activated by:	CRP
Repressed by:	NagC

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_811	706185		forward	nd	[RS-EPT-CBR]	[4]
	promoter	TSS_812	706250		reverse	nd	[RS-EPT-CBR]	[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Soysa HSM., Schulte A., Suginta W., 2017, Functional analysis of an unusual porin-like channel that imports chitin for alternative carbon metabolism in Escherichia coli., J Biol Chem 292(47):19328-19337
[2] Soysa HSM., Suginta W., Moonsap W., Smith MF., 2018, Chitosugar translocation by an unexpressed monomeric protein channel., Phys Rev E 97(5-1):052417
[3] Yang C., Rodionov DA., Li X., Laikova ON., Gelfand MS., Zagnitko OP., Romine MF., Obraztsova AY., Nealson KH., Osterman AL., 2006, Comparative genomics and experimental characterization of N-acetylglucosamine utilization pathway of Shewanella oneidensis., J Biol Chem 281(40):29872-85
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.