RegulonDB RegulonDB 10.7: Gene Form
   

mdfA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mdfA ybjG ybjH PhoP cmrp cmrp cmrp2 cmrp2 ybjGp ybjGp TSS_990 TSS_990

Gene      
Name: mdfA    Texpresso search in the literature
Synonym(s): ECK0832, G6440, b0842, cmlA, cmr
Genome position(nucleotides): 883673 --> 884905 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.39
External database links:  
ASAP:
ABE-0002871
ECHOBASE:
EB3460
OU-MICROARRAY:
b0842
PORTECO:
cmr
REGULONDB:
b0842
STRING:
511145.b0842
M3D: mdfA
COLOMBOS: mdfA
PortEco: b0842


Product      
Name: multidrug efflux pump MdfA / Na+:H+ antiporter / K+:H+ antiporter
Synonym(s): CmlA, Cmr, MdfA
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 44.321
Isoelectric point: 9.092
Motif(s):
 
Type Positions Sequence
26 -> 39 EFSTYIGNDMIQPG
73 -> 83 GPLSDRIGRRP
108 -> 116 FTLLRFLQG
146 -> 146 M
227 -> 247 ALGFVSLPLLAWIAQSPIIII

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
  5 - cell processes --> 5.6 - protection --> 5.6.4 - drug resistance/sensitivity
  6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)  
cellular_component GO:0005623 - cell
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
molecular_function GO:0022857 - transmembrane transporter activity
GO:0015299 - solute:proton antiporter activity
GO:0015385 - sodium:proton antiporter activity
GO:0015386 - potassium:proton antiporter activity
biological_process GO:1990961 - xenobiotic detoxification by transmembrane export across the plasma membrane
GO:0046677 - response to antibiotic
GO:0030641 - regulation of cellular pH
GO:0006855 - drug transmembrane transport
GO:0055085 - transmembrane transport
GO:0035725 - sodium ion transmembrane transport
GO:0071805 - potassium ion transmembrane transport
GO:0098656 - anion transmembrane transport
Note(s): Note(s): ...[more].
Reference(s): [1] Alon Cudkowicz N., et al., 2019
[2] Edgar R., et al., 1999
[3] Heng J., et al., 2015
[4] Jaenecke F., et al., 2018
[5] Lee A., et al., 2000
[6] Nagarathinam K., et al., 2017
[7] Schuldiner S. 2018
[8] Shuster Y., et al., 2016
[9] Sigal N., et al., 2007
[10] Wu HH., et al., 2019
[11] Zomot E., et al., 2018
External database links:  
DIP:
DIP-48116N
ECOCYC:
CMR-MONOMER
ECOLIWIKI:
b0842
INTERPRO:
IPR011701
INTERPRO:
IPR005829
INTERPRO:
IPR036259
INTERPRO:
IPR020846
MINT:
MINT-1227586
MODBASE:
P0AEY8
PDB:
6GV1
PDB:
6EUQ
PDB:
4ZP2
PDB:
4ZP0
PDB:
4ZOW
PFAM:
PF07690
PRIDE:
P0AEY8
PRODB:
PRO_000022304
PROSITE:
PS50850
PROTEINMODELPORTAL:
P0AEY8
REFSEQ:
NP_415363
SMR:
P0AEY8
UNIPROT:
P0AEY8


Operon      
Name: mdfA         
Operon arrangement:
Transcription unit        Promoter
cmr
cmr


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_990 883505 reverse nd [RS-EPT-CBR] [12]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Alon Cudkowicz N., Schuldiner S., 2019, Deletion of the major Escherichia coli multidrug transporter AcrB reveals transporter plasticity and redundancy in bacterial cells., PLoS One 14(6):e0218828

 [2] Edgar R., Bibi E., 1999, A single membrane-embedded negative charge is critical for recognizing positively charged drugs by the Escherichia coli multidrug resistance protein MdfA., EMBO J 18(4):822-32

 [3] Heng J., Liu M., Liu Y., Fan J., Wang X., Zhao Y., Zhang XC., 2015, Substrate-bound structure of the E. coli multidrug resistance transporter MdfA., Cell Res 25(9):1060-73

 [4] Jaenecke F., Nakada-Nakura Y., Nagarathinam K., Ogasawara S., Liu K., Hotta Y., Iwata S., Nomura N., Tanabe M., 2018, Generation of Conformation-Specific Antibody Fragments for Crystallization of the Multidrug Resistance Transporter MdfA., Methods Mol Biol 1700:97-109

 [5] Lee A., Mao W., Warren MS., Mistry A., Hoshino K., Okumura R., Ishida H., Lomovskaya O., 2000, Interplay between efflux pumps may provide either additive or multiplicative effects on drug resistance., J Bacteriol 182(11):3142-50

 [6] Nagarathinam K., Jaenecke F., Nakada-Nakura Y., Hotta Y., Liu K., Iwata S., Stubbs MT., Nomura N., Tanabe M., 2017, The multidrug-resistance transporter MdfA from Escherichia coli: crystallization and X-ray diffraction analysis., Acta Crystallogr F Struct Biol Commun 73(Pt 7):423-430

 [7] Schuldiner S., 2018, The Escherichia coli effluxome., Res Microbiol 169(7-8):357-362

 [8] Shuster Y., Steiner-Mordoch S., Alon Cudkowicz N., Schuldiner S., 2016, A Transporter Interactome Is Essential for the Acquisition of Antimicrobial Resistance to Antibiotics., PLoS One 11(4):e0152917

 [9] Sigal N., Lewinson O., Wolf SG., Bibi E., 2007, E. coli multidrug transporter MdfA is a monomer., Biochemistry 46(17):5200-8

 [10] Wu HH., Symersky J., Lu M., 2019, Structure of an engineered multidrug transporter MdfA reveals the molecular basis for substrate recognition., Commun Biol 2:210

 [11] Zomot E., Yardeni EH., Vargiu AV., Tam HK., Malloci G., Ramaswamy VK., Perach M., Ruggerone P., Pos KM., Bibi E., 2018, A New Critical Conformational Determinant of Multidrug Efflux by an MFS Transporter., J Mol Biol 430(9):1368-1385

 [12] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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