RegulonDB RegulonDB 10.8: Gene Form
   

lolA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ftsK rarA lolA RcsB terminator TSS_1082 TSS_1082 TSS_1081 TSS_1081 lolAp lolAp TSS_1079 TSS_1079 TSS_1078 TSS_1078 TSS_1077 TSS_1077 TSS_1076 TSS_1076 TSS_1075 TSS_1075 TSS_1074 TSS_1074 TSS_1073 TSS_1073 TSS_1072 TSS_1072 TSS_1071 TSS_1071 TSS_1070 TSS_1070 TSS_1069 TSS_1069 TSS_1068 TSS_1068 TSS_1067 TSS_1067 TSS_1066 TSS_1066 TSS_1065 TSS_1065

Gene      
Name: lolA    Texpresso search in the literature
Synonym(s): ECK0882, G6465, b0891, lplA, yzzV
Genome position(nucleotides): 937372 --> 937983 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
50.98
External database links:  
ASAP:
ABE-0003031
ECHOBASE:
EB2548
OU-MICROARRAY:
b0891
PortEco:
lolA
STRING:
511145.b0891
COLOMBOS: lolA


Product      
Name: outer membrane lipoprotein carrier protein
Synonym(s): LolA, LplA, YzzV, p20
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space
Molecular weight: 22.497
Isoelectric point: 6.795
Motif(s):
 
Type Positions Sequence
77 -> 83 ESILVSD
118 -> 123 QSSDWQ
127 -> 127 I
148 -> 148 F
31 -> 31 L

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport
Gene Ontology Terms (GO)  
cellular_component GO:0030288 - outer membrane-bounded periplasmic space
GO:0042597 - periplasmic space
molecular_function GO:0005515 - protein binding
biological_process GO:0015031 - protein transport
GO:0042953 - lipoprotein transport
GO:0044874 - lipoprotein localization to outer membrane
GO:0072323 - chaperone-mediated protein transport across periplasmic space
Note(s): Note(s): ...[more].
Evidence: [APPHINH] Assay of protein purified to homogeneity from its native host
Reference(s): [1] Matsuyama S., et al., 1995
[2] Okuda S., et al., 2008
[3] Taniguchi N., et al., 2005
[4] Taniguchi N., et al., 2008
[5] Tao K., et al., 2010
[6] Watanabe S., et al., 2008
[7] Watanabe S., et al., 2007
External database links:  
DIP:
DIP-35675N
ECOCYC:
G6465-MONOMER
ECOLIWIKI:
b0891
INTERPRO:
IPR029046
INTERPRO:
IPR018323
INTERPRO:
IPR004564
MINT:
P61316
MODBASE:
P61316
PANTHER:
PTHR35869
PDB:
6F3Z
PDB:
6FHM
PDB:
2ZPD
PDB:
2ZPC
PDB:
1UA8
PDB:
1IWL
PDB:
3KSN
PFAM:
PF03548
PRIDE:
P61316
PRODB:
PRO_000023103
REFSEQ:
NP_415411
SMR:
P61316
UNIPROT:
P61316


Operon      
Name: lolA-rarA         
Operon arrangement:
Transcription unit        Promoter
lolA-rarA


Transcriptional Regulation      
Display Regulation             
Activated by: RcsB


RNA cis-regulatory element    
Attenuation: Translational


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1065 933716 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1066 934543 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1067 934547 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1068 934549 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1069 935748 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1070 936467 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1071 936562 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1072 936567 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1073 936589 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1074 936705 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1075 936711 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1076 936713 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1077 936735 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1078 936737 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1079 936755 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1081 937877 forward nd [RS-EPT-CBR] [8]
  promoter TSS_1082 937932 forward nd [RS-EPT-CBR] [8]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Matsuyama S., Tajima T., Tokuda H., 1995, A novel periplasmic carrier protein involved in the sorting and transport of Escherichia coli lipoproteins destined for the outer membrane., EMBO J 14(14):3365-72

 [2] Okuda S., Watanabe S., Tokuda H., 2008, A short helix in the C-terminal region of LolA is important for the specific membrane localization of lipoproteins., FEBS Lett 582(15):2247-51

 [3] Taniguchi N., Matsuyama S., Tokuda H., 2005, Mechanisms underlying energy-independent transfer of lipoproteins from LolA to LolB, which have similar unclosed {beta}-barrel structures., J Biol Chem 280(41):34481-8

 [4] Taniguchi N., Tokuda H., 2008, Molecular events involved in a single cycle of ligand transfer from an ATP binding cassette transporter, LolCDE, to a molecular chaperone, LolA., J Biol Chem 283(13):8538-44

 [5] Tao K., Watanabe S., Narita S., Tokuda H., 2010, A periplasmic LolA derivative with a lethal disulfide bond activates the Cpx stress response system., J Bacteriol 192(21):5657-62

 [6] Watanabe S., Oguchi Y., Takeda K., Miki K., Tokuda H., 2008, Introduction of a lethal redox switch that controls the opening and closing of the hydrophobic cavity in LolA., J Biol Chem 283(37):25421-7

 [7] Watanabe S., Oguchi Y., Yokota N., Tokuda H., 2007, Large-scale preparation of the homogeneous LolA lipoprotein complex and efficient in vitro transfer of lipoproteins to the outer membrane in a LolB-dependent manner., Protein Sci 16(12):2741-9

 [8] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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