RegulonDB

Gene
Name:	pgaA
Synonym(s):	ECK1014, G6531, b1024, hmsH, ycdS
Genome position(nucleotides):	1089866 <-- 1092289 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	47.32
External database links:

ASAP:	ABE-0003469
ECHOBASE:	EB3625
OU-MICROARRAY:	b1024
PortEco:	pgaA
STRING:	511145.b1024
COLOMBOS:	pgaA

Product

Name:

partially deacetylated poly-β-1,6-N-acetyl-D-glucosamine outer membrane porin

Synonym(s):

HmsH, PgaA, YcdS

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

outer membrane

Molecular weight:

92.207

Isoelectric point:

6.561

Motif(s):

Type	Positions	Sequence
	165 -> 198	KANLLAEAYIYKLAGRHQDELRAMTESLPENAST
	27 -> 807	SPAAKSAVNNAYDALIIEARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYAAVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNSGAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAIDDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQYQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQSIMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTPTSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADGQFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVEFDMTFRF
	279 -> 311	RIQVDHLGALLTRDRYKDVISHYQRLKKTGQII
	98 -> 131	ARGYAAVAVAYRNLQQWQNSLTLWQKALSLEPQN
	1 -> 26	MYSSSRKRCPKTKWALKLLTAAFLAA

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		4 - transport --> 4.1 - Channel-type Transporters --> 4.1.B - Beta barrel porins (The Outer Membrane Porin (OMP) Functional Superfamily)
Gene Ontology Terms (GO)
	cellular_component	GO:0009279 - cell outer membrane GO:0016020 - membrane GO:0016021 - integral component of membrane GO:0045203 - integral component of cell outer membrane
	molecular_function	GO:1901515 - poly-beta-1,6-N-acetyl-D-glucosamine transmembrane transporter activity GO:0015159 - polysaccharide transmembrane transporter activity
	biological_process	GO:0015774 - polysaccharide transport GO:0055085 - transmembrane transport GO:0044010 - single-species biofilm formation

Note(s):

Note(s): ...[more].

Reference(s):

[1] Agladze K., et al., 2005
[2] Samoilova Z., et al., 2019
[3] Wang Y., et al., 2016

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

PDB:

PRIDE:

PRODB:

PROSITE:

PROSITE:

REFSEQ:

SMART:

SMR:

UNIPROT:

Operon

Name:

pgaABCD

Operon arrangement:

Transcription unit	Promoter
pgaABCD	pgaAp

Transcriptional Regulation
	Display Regulation
Activated by:	NhaR
Repressed by:	OmpR

Regulation by small RNA
	Display Regulation
small RNA	mcaS

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_1319	1092840		forward	nd	[RS-EPT-CBR]	[4]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Agladze K., Wang X., Romeo T., 2005, Spatial periodicity of Escherichia coli K-12 biofilm microstructure initiates during a reversible, polar attachment phase of development and requires the polysaccharide adhesin PGA., J Bacteriol 187(24):8237-46
[2] Samoilova Z., Tyulenev A., Muzyka N., Smirnova G., Oktyabrsky O., 2019, Tannic and gallic acids alter redox-parameters of the medium and modulate biofilm formation., AIMS Microbiol 5(4):379-392
[3] Wang Y., Andole Pannuri A., Ni D., Zhou H., Cao X., Lu X., Romeo T., Huang Y., 2016, Structural Basis for Translocation of a Biofilm-supporting Exopolysaccharide across the Bacterial Outer Membrane., J Biol Chem 291(19):10046-57
[4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.