RegulonDB

Gene
Name:	murJ
Synonym(s):	ECK1054, G6561, b1069, mviN, yceN
Genome position(nucleotides):	1127839 --> 1129374 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	53.71
External database links:

ASAP:	ABE-0003630
ECHOBASE:	EB3639
OU-MICROARRAY:	b1069
PortEco:	murJ
STRING:	511145.b1069
COLOMBOS:	murJ

Gene

Name:

murJ

Synonym(s):

ECK1054, G6561, b1069, mviN, yceN

Genome position(nucleotides):

1127839 --> 1129374 Genome Browser

Strand:

forward

Sequence:

Get nucleotide sequence FastaFormat

GC content %:

53.71

External database links:

ASAP:

ECHOBASE:

OU-MICROARRAY:

PortEco:

STRING:

COLOMBOS:

Type

Positions

Sequence

18 -> 18

409 -> 429

GLSLSIGLAACLNASLLYWQL

6 -> 32

SLAAVSSMTMFSRVLGFARDAIVARIF

39 -> 39

52 -> 52

Type

Positions

Sequence

18 -> 18

409 -> 429

GLSLSIGLAACLNASLLYWQL

6 -> 32

SLAAVSSMTMFSRVLGFARDAIVARIF

39 -> 39

52 -> 52

186 -> 207

VLALAWAVTVGGVLQLVYQLPH

224 -> 253

AGAMRVVKQMGPAILGVSVSQISLIINTIF

28 -> 478

VARIFGAGMATDAFFVAFKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAGMLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIPAFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPRINFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPSGVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTVSLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLILTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVVAVLVMSGVLLGMLHIMPEWSLGTMPWRL

41 -> 69

FFVAFKLPNLLRRIFAEGAFSQAFVPILA

444 -> 463

FLLRLVVAVLVMSGVLLGML

157 -> 178

FSIPAFAPTLLNISMIGFALFA

264 -> 292

WMYYADRLMEFPSGVLGVALGTILLPSLS

303 -> 337

YNRLMDWGLRLCFLLALPSAVALGILSGPLTVSLF

347 -> 374

ALMTQRALIAYSVGLIGLIVVKVLAPGF

478 -> 499

LLRLMAVVLAGIAAYFAALAVL

83 -> 109

FVSYVSGLLTLALAVVTVAGMLAAPWV

124 -> 154

FALTSQLLKITFPYILLISLASLVGAILNTW

380 -> 402

IKTPVKIAIVTLILTQLMNLAFI

270 -> 270

410 -> 429

LSLSIGLAACLNASLLYWQL

499 -> 499

24 -> 24

Multifun Terms (GenProtEC)

4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)

6 - cell structure --> 6.1 - membrane

Gene Ontology Terms (GO)

cellular_component

GO:0000935 - division septum
GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane

molecular_function

GO:1901612 - cardiolipin binding
GO:0015648 - lipid-linked peptidoglycan transporter activity

biological_process

GO:0009252 - peptidoglycan biosynthetic process
GO:0008360 - regulation of cell shape
GO:0034204 - lipid translocation
GO:0071555 - cell wall organization
GO:0015836 - lipid-linked peptidoglycan transport
GO:0034203 - glycolipid translocation

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_1341	1127783		forward	nd	[RS-EPT-CBR]	[7]

Type

Name

Post Left

Post Right

Strand

Notes

Evidence (Confirmed, Strong, Weak)

References

promoter

TSS_1341

1127783

forward

[RS-EPT-CBR]

[7]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Evidence

[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Afset JE., Bruant G., Brousseau R., Harel J., Anderssen E., Bevanger L., Bergh K., 2006, Identification of virulence genes linked with diarrhea due to atypical enteropathogenic Escherichia coli by DNA microarray analysis and PCR., J Clin Microbiol 44(10):3703-11
[2] Bruant G., Maynard C., Bekal S., Gaucher I., Masson L., Brousseau R., Harel J., 2006, Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli., Appl Environ Microbiol 72(5):3780-4
[3] Elhenawy W., Davis RM., Fero J., Salama NR., Felman MF., Ruiz N., 2016, The O-Antigen Flippase Wzk Can Substitute for MurJ in Peptidoglycan Synthesis in Helicobacter pylori and Escherichia coli., PLoS One 11(8):e0161587
[4] Lerat E., Daubin V., Moran NA., 2003, From gene trees to organismal phylogeny in prokaryotes: the case of the gamma-Proteobacteria., PLoS Biol 1(1):E19
[5] MacLeod D., Charlebois RL., Doolittle F., Bapteste E., 2005, Deduction of probable events of lateral gene transfer through comparison of phylogenetic trees by recursive consolidation and rearrangement., BMC Evol Biol 5(1):27
[6] Price MN., Huang KH., Arkin AP., Alm EJ., 2005, Operon formation is driven by co-regulation and not by horizontal gene transfer., Genome Res 15(6):809-19
[7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

Reference(s)

[1] Afset JE., Bruant G., Brousseau R., Harel J., Anderssen E., Bevanger L., Bergh K., 2006, Identification of virulence genes linked with diarrhea due to atypical enteropathogenic Escherichia coli by DNA microarray analysis and PCR., J Clin Microbiol 44(10):3703-11

[2] Bruant G., Maynard C., Bekal S., Gaucher I., Masson L., Brousseau R., Harel J., 2006, Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli., Appl Environ Microbiol 72(5):3780-4

[3] Elhenawy W., Davis RM., Fero J., Salama NR., Felman MF., Ruiz N., 2016, The O-Antigen Flippase Wzk Can Substitute for MurJ in Peptidoglycan Synthesis in Helicobacter pylori and Escherichia coli., PLoS One 11(8):e0161587

[4] Lerat E., Daubin V., Moran NA., 2003, From gene trees to organismal phylogeny in prokaryotes: the case of the gamma-Proteobacteria., PLoS Biol 1(1):E19

[5] MacLeod D., Charlebois RL., Doolittle F., Bapteste E., 2005, Deduction of probable events of lateral gene transfer through comparison of phylogenetic trees by recursive consolidation and rearrangement., BMC Evol Biol 5(1):27

[6] Price MN., Huang KH., Arkin AP., Alm EJ., 2005, Operon formation is driven by co-regulation and not by horizontal gene transfer., Genome Res 15(6):809-19

[7] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.