RegulonDB RegulonDB 10.8: Gene Form
   

nagK gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

lolE nagK cobB TSS_1455 TSS_1455 nagKp3 nagKp3 TSS_1454 TSS_1454 TSS_1453 TSS_1453 TSS_1452 TSS_1452

Gene      
Name: nagK    Texpresso search in the literature
Synonym(s): ECK1105, G6576, b1119, ycfX
Genome position(nucleotides): 1178593 --> 1179504 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 52.74
External database links:  
ASAP:
 ABE-0003778
ECHOBASE:
 EB3216
OU-MICROARRAY:
 b1119
PortEco:
 nagK
STRING:
 511145.b1119
COLOMBOS: nagK


Product      
Name: N-acetyl-D-glucosamine kinase
Synonym(s): NagK, YcfX
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 33.043
Isoelectric point: 5.156
Motif(s):
 
Type Positions Sequence
4 -> 11 GFDIGGTK
133 -> 140 GVGGGLIF
2 -> 300 YYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVCELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRLDNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRLPVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQGDEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPVARVPRIERARHGDAGGMRGAAFLH

 
Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.34 - murein turnover, recycling
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016740 - transferase activity
GO:0046872 - metal ion binding
GO:0016301 - kinase activity
GO:0000166 - nucleotide binding
GO:0005524 - ATP binding
GO:0008270 - zinc ion binding
GO:0045127 - N-acetylglucosamine kinase activity
biological_process GO:0005975 - carbohydrate metabolic process
GO:0009254 - peptidoglycan turnover
GO:0006044 - N-acetylglucosamine metabolic process
GO:0016310 - phosphorylation
GO:0046835 - carbohydrate phosphorylation
Note(s): Note(s): ...[more].
Reference(s): [1] Toba FA., et al., 2011
External database links:  
DIP:
 DIP-11548N
ECOCYC:
 G6576-MONOMER
ECOLIWIKI:
 b1119
INTERPRO:
 IPR000600
INTERPRO:
 IPR023505
MINT:
 MINT-1274945
MODBASE:
 P75959
PANTHER:
 PTHR18964
PFAM:
 PF00480
PRIDE:
 P75959
PROSITE:
 PS01125
REFSEQ:
 NP_415637
SMR:
 P75959
SWISSMODEL:
 P75959
UNIPROT:
 P75959


Operon      
Name: nagK-cobB         
Operon arrangement:
Transcription unit        Promoter
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1452 1177616 forward nd [RS-EPT-CBR] [2]
  promoter TSS_1453 1178231 forward nd [RS-EPT-CBR] [2]
  promoter TSS_1454 1178238 forward nd [RS-EPT-CBR] [2]
  promoter nagKp3 1178540 forward Similarity to the consensus
Read more > 		[ICWHO] [3]
  promoter TSS_1455 1179908 forward nd [RS-EPT-CBR] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight


Reference(s)    

 [1] Toba FA., Thompson MG., Campbell BR., Junker LM., Rueggeberg KG., Hay AG., 2011, Role of DLP12 lysis genes in Escherichia coli biofilm formation., Microbiology 157(Pt 6):1640-50

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [3] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

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