RegulonDB RegulonDB 10.8: Gene Form
   

puuD gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

puuA puuD puuR ArcA PuuR CRP PuuR PuuR PuuR FNR TSS_1741 TSS_1741 puuDp puuDp puuAp puuAp

Gene      
Name: puuD    Texpresso search in the literature
Synonym(s): ECK1293, G6645, b1298, ycjL
Genome position(nucleotides): 1361120 --> 1361884 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
52.29
External database links:  
ASAP:
ABE-0004369
ECHOBASE:
EB3668
OU-MICROARRAY:
b1298
PortEco:
puuD
STRING:
511145.b1298
COLOMBOS: puuD


Product      
Name: γ-glutamyl-γ-aminobutyrate hydrolase
Synonym(s): PuuD, YcjL
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 28.013
Isoelectric point: 5.499
Motif(s):
 
Type Positions Sequence
16 -> 250 RNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINAALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRILFEGFITACQHHIAE
8 -> 224 PVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINAALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPE
114 -> 114 C

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.4 - amines
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0016787 - hydrolase activity
GO:0033969 - gamma-glutamyl-gamma-aminobutyrate hydrolase activity
biological_process GO:0006598 - polyamine catabolic process
GO:0009447 - putrescine catabolic process
Note(s): Note(s): ...[more].
External database links:  
ECOCYC:
G6645-MONOMER
ECOLIWIKI:
b1298
INTERPRO:
IPR029062
INTERPRO:
IPR017926
INTERPRO:
IPR011697
MODBASE:
P76038
PFAM:
PF07722
PRIDE:
P76038
PRODB:
PRO_000023652
PROSITE:
PS51273
REFSEQ:
NP_415814
SMR:
P76038
UNIPROT:
P76038


Operon      
Name: puuDRCBE         
Operon arrangement:
Transcription unit        Promoter
puuDRCBE
puuCBE


Transcriptional Regulation      
Display Regulation             
Repressed by: CRP, PuuR, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1741 1361667 forward nd [RS-EPT-CBR] [1]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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