RegulonDB RegulonDB 10.8: Gene Form
   

puuE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

puuB puuE pspF PspF

Gene      
Name: puuE    Texpresso search in the literature
Synonym(s): ECK1297, G6646, b1302, goaG
Genome position(nucleotides): 1365550 --> 1366815 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
57.66
External database links:  
ASAP:
ABE-0004380
ECHOBASE:
EB2979
OU-MICROARRAY:
b1302
PortEco:
puuE
STRING:
511145.b1302
COLOMBOS: puuE


Product      
Name: 4-aminobutyrate aminotransferase PuuE
Synonym(s): GoaG, PuuE
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 44.729
Isoelectric point: 6.135
Motif(s):
 
Type Positions Sequence
21 -> 419 MCNFFAQSAENATLKDVEGNEYIDFAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQAKTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGPFPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPKELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLSGVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTLIDAKESVPAIAAVRGLGSMIAVEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYGNVIRFLYPLTIPDAQFDAAMKILQDAL
110 -> 111 GA
267 -> 267 K
238 -> 241 DEVQ

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.4 - amines
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0034386 - 4-aminobutyrate:2-oxoglutarate transaminase activity
GO:0003824 - catalytic activity
GO:0016740 - transferase activity
GO:0008483 - transaminase activity
GO:0030170 - pyridoxal phosphate binding
GO:0003867 - 4-aminobutyrate transaminase activity
biological_process GO:0009447 - putrescine catabolic process
GO:0009448 - gamma-aminobutyric acid metabolic process
GO:0009450 - gamma-aminobutyric acid catabolic process
Note(s): Note(s): ...[more].
Reference(s): [1] Pham VD., et al., 2016
[2] Pham VD., et al., 2016
[3] Zhao A., et al., 2017
External database links:  
DIP:
DIP-9825N
ECOCYC:
G6646-MONOMER
ECOLIWIKI:
b1302
INTERPRO:
IPR015421
INTERPRO:
IPR005814
INTERPRO:
IPR015422
INTERPRO:
IPR015424
INTERPRO:
IPR004632
MINT:
MINT-1256713
MODBASE:
P50457
PANTHER:
PTHR11986
PFAM:
PF00202
PRIDE:
P50457
PROSITE:
PS00600
REFSEQ:
NP_415818
SMR:
P50457
SWISSMODEL:
P50457
UNIPROT:
P50457


Operon      
Name: puuDRCBE         
Operon arrangement:
Transcription unit        Promoter
puuDRCBE
puuCBE


Transcriptional Regulation      
Display Regulation             
Repressed by: CRP, PuuR, ArcA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Pham VD., Somasundaram S., Lee SH., Park SJ., Hong SH., 2016, Engineering the intracellular metabolism of Escherichia coli to produce gamma-aminobutyric acid by co-localization of GABA shunt enzymes., Biotechnol Lett 38(2):321-7

 [2] Pham VD., Somasundaram S., Lee SH., Park SJ., Hong SH., 2016, Redirection of Metabolic Flux into Novel Gamma-Aminobutyric Acid Production Pathway by Introduction of Synthetic Scaffolds Strategy in Escherichia Coli., Appl Biochem Biotechnol 178(7):1315-24

 [3] Zhao A., Hu X., Wang X., 2017, Metabolic engineering of Escherichia coli to produce gamma-aminobutyric acid using xylose., Appl Microbiol Biotechnol 101(9):3587-3603


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