RegulonDB

Gene
Name:	gadC
Synonym(s):	ECK1486, G6786, acsA, b1492, xasA
Genome position(nucleotides):	1568954 <-- 1570489 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	48.11
External database links:

ASAP:	ABE-0004973
ECHOBASE:	EB2350
OU-MICROARRAY:	b1492
PortEco:	gadC
STRING:	511145.b1492
COLOMBOS:	gadC

Product

Name:

L-glutamate:4-aminobutyrate antiporter

Synonym(s):

AcsA, GadC, GadC GABA APC transporter, XasA, glutamic acid:gamma;-aminobutyrate antiporter

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

55.077

Isoelectric point:

8.902

Motif(s):

Type	Positions	Sequence
	42 -> 62	LVFFLLLGGILWFIPVGLCAA
	94 -> 114	FGYLQIAIGFIPMLYFVLGAL
	292 -> 312	VISALLLLGVLAEIASWIVGP
	158 -> 178	VGFFAGILLPAFILIALAAIY
	13 -> 451	LTLLGFFAITASMVMAVYEYPTFATSGFSLVFFLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIGFIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGFFAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVEASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFTVLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNKNGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVLVLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLVV

Classification:

Multifun Terms (GenProtEC)
		4 - transport --> 4.2 - Electrochemical potential driven transporters --> 4.2.A - Porters (Uni-, Sym- and Antiporters)
		6 - cell structure --> 6.1 - membrane
Gene Ontology Terms (GO)
	cellular_component	GO:0005623 - cell GO:0016020 - membrane GO:0005886 - plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0015297 - antiporter activity GO:0022857 - transmembrane transporter activity
	biological_process	GO:0006865 - amino acid transport GO:0051454 - intracellular pH elevation GO:0055085 - transmembrane transport

Note(s):

Note(s): ...[more].

Reference(s):

[1] De Biase D., et al., 1999
[2] Lin J., et al., 1995
[3] Tramonti A., et al., 2006

External database links:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PANTHER:

PDB:

PDB:

PFAM:

PRIDE:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

gadBC

Operon arrangement:

Transcription unit	Promoter
gadBC	gadBp

Transcriptional Regulation
	Display Regulation
Activated by:	GadX, GadW, AdiY, GadE, RcsB
Repressed by:	CRP, FliZ, GadW, Fis

RNA cis-regulatory element

Attenuation:	Translational

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	yddWp6	1568852	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.893. P-value: 3.330e-5 Read more >	[ICWHO]	[4]
promoter	gadCp5	1570527	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.07. P-value: 9.142e-5 Read more >	[ICWHO]	[4]
promoter	gadCp6	1570577	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.46. P-value: 6.067e-5 Read more >	[ICWHO]	[4]
promoter	gadCp1	1570584	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: poor homology. Score: -3.4. P-value: 9.611e-5 Read more >	[ICWHO]	[4]
promoter	TSS_1834	1571620	reverse	nd	[RS-EPT-CBR]	[5]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] De Biase D., Tramonti A., Bossa F., Visca P., 1999, The response to stationary-phase stress conditions in Escherichia coli: role and regulation of the glutamic acid decarboxylase system., Mol Microbiol 32(6):1198-211
[2] Lin J., Lee IS., Frey J., Slonczewski JL., Foster JW., 1995, Comparative analysis of extreme acid survival in Salmonella typhimurium, Shigella flexneri, and Escherichia coli., J Bacteriol 177(14):4097-104
[3] Tramonti A., De Canio M., Delany I., Scarlato V., De Biase D., 2006, Mechanisms of transcription activation exerted by GadX and GadW at the gadA and gadBC gene promoters of the glutamate-based acid resistance system in Escherichia coli., J Bacteriol 188(23):8118-27
[4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.