RegulonDB RegulonDB 10.7: Gene Form
   

clcB gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

clcB mlc ynfK dmsD ynfKp6 ynfKp6 TSS_1877 TSS_1877 ynfKp5 ynfKp5 TSS_1876 (cluster) TSS_1876 (cluster)

Gene      
Name: clcB    Texpresso search in the literature
Synonym(s): ECK1587, G6850, b1592, mriT, ynfJ
Genome position(nucleotides): 1665315 --> 1666571 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
54.26
External database links:  
ASAP:
ABE-0005317
ECHOBASE:
EB3609
MIM:
160800
MIM:
255700
OU-MICROARRAY:
b1592
PORTECO:
clcB
REGULONDB:
b1592
STRING:
511145.b1592
M3D: clcB
COLOMBOS: clcB
PortEco: b1592


Product      
Name: putative chloride:H+ antiporter ClcB
Synonym(s): ClC-ec2, ClcB, MriT, YnfJ
Sequence: Get amino acid sequence Fasta Format
Cellular location: inner membrane
Molecular weight: 44.153
Isoelectric point: 8.214
Motif(s):
 
Type Positions Sequence
291 -> 311 APPLLMIIAGIFLCKLCAVLA
352 -> 372 LLLGLTGMATLLAATTHAPIM
2 -> 22 FHRLLIATVVGILAAFAVAGF
146 -> 166 LWIACGAAAGMAAAYRAPLAG
380 -> 400 MTGEYQLLPGLLIACVIASVI

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport
Gene Ontology Terms (GO)  
cellular_component GO:0016020 - membrane
GO:0005886 - plasma membrane
GO:0005887 - integral component of plasma membrane
GO:0016021 - integral component of membrane
GO:0034707 - chloride channel complex
molecular_function GO:0005244 - voltage-gated ion channel activity
GO:0005247 - voltage-gated chloride channel activity
GO:0005254 - chloride channel activity
biological_process GO:0006811 - ion transport
GO:0006821 - chloride transport
GO:0055085 - transmembrane transport
GO:0010447 - response to acidic pH
GO:0071468 - cellular response to acidic pH
GO:0034765 - regulation of ion transmembrane transport
GO:1902476 - chloride transmembrane transport
Note(s): Note(s): ...[more].
Evidence: [HIFS] Human inference of function from sequence
Reference(s): [1] Jentsch TJ., et al., 2002
[2] Maduke M., et al., 1999
External database links:  
DIP:
DIP-28065N
ECOCYC:
YNFJ-MONOMER
ECOLIWIKI:
b1592
INTERPRO:
IPR001807
INTERPRO:
IPR014743
INTERPRO:
IPR023790
MODBASE:
P76175
PFAM:
PF00654
PRIDE:
P76175
PRINTS:
PR00762
PRODB:
PRO_000022295
PROTEINMODELPORTAL:
P76175
REFSEQ:
NP_416109
SMR:
P76175
UNIPROT:
P76175


Operon      
Name: clcB         
Operon arrangement:
Transcription unit        Promoter
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_1876 (cluster) 1667036 reverse For this promoter, there
Read more >
[RS-EPT-CBR] [3]
  promoter ynfKp5 1667240 reverse Similarity to the consensus
Read more >
[ICWHO] [4]
  promoter TSS_1877 1667270 reverse nd [RS-EPT-CBR] [3]
  promoter ynfKp6 1667335 reverse Similarity to the consensus
Read more >
[ICWHO] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Jentsch TJ., Stein V., Weinreich F., Zdebik AA., 2002, Molecular structure and physiological function of chloride channels., Physiol Rev 82(2):503-68

 [2] Maduke M., Pheasant DJ., Miller C., 1999, High-level expression, functional reconstitution, and quaternary structure of a prokaryotic ClC-type chloride channel., J Gen Physiol 114(5):713-22

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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