RegulonDB RegulonDB 10.8: Gene Form
   

dmlA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

yeaV dmlA dmlR terminator anti-terminator anti-anti-terminator anti-terminator TSS_2129 TSS_2129 dmlRp3 dmlRp3 dmlAp4 dmlAp4

Gene      
Name: dmlA    Texpresso search in the literature
Synonym(s): ECK1798, G6986, b1800, ttuC, yeaU
Genome position(nucleotides): 1881912 --> 1882997 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
53.68
External database links:  
ASAP:
ABE-0005991
ECHOBASE:
EB3280
OU-MICROARRAY:
b1800
PortEco:
yeaU
STRING:
511145.b1800
COLOMBOS: dmlA


Product      
Name: D-malate/3-isopropylmalate dehydrogenase (decarboxylating)
Synonym(s): DmlA, TtuC, YeaU
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 40.315
Isoelectric point: 5.13
Motif(s):
 
Type Positions Sequence
7 -> 354 IAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGNATTPQVADA
88 -> 98 SLWGSLLKFRR

 

Classification:
Multifun Terms (GenProtEC)  
  1 - metabolism --> 1.1 - carbon utilization --> 1.1.1 - carbon compounds
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0016491 - oxidoreductase activity
GO:0003862 - 3-isopropylmalate dehydrogenase activity
GO:0016616 - oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0046553 - D-malate dehydrogenase (decarboxylating) activity
GO:0000287 - magnesium ion binding
GO:0009027 - tartrate dehydrogenase activity
GO:0051287 - NAD binding
biological_process GO:0006108 - malate metabolic process
GO:0055114 - oxidation-reduction process
Note(s): Note(s): ...[more].
External database links:  
DIP:
DIP-11800N
ECOCYC:
G6986-MONOMER
ECOLIWIKI:
b1800
INTERPRO:
IPR011829
INTERPRO:
IPR024084
INTERPRO:
IPR019818
MINT:
MINT-1273575
MODBASE:
P76251
PANTHER:
PTHR11835
PFAM:
PF00180
PRIDE:
P76251
PROSITE:
PS00470
REFSEQ:
NP_416314
SMART:
SM01329
SMR:
P76251
UNIPROT:
P76251


Operon      
Name: dmlA         
Operon arrangement:
Transcription unit        Promoter
 


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter dmlAp4 1881712 forward Similarity to the consensus
Read more >
[ICWHO] [1]
  promoter dmlRp3 1881855 reverse Similarity to the consensus
Read more >
[ICWHO] [1]
  promoter TSS_2129 1881877 forward nd [RS-EPT-CBR] [2]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [2] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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