RegulonDB RegulonDB 10.6.3: Gene Form
   

znuA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

mepM znuA znuC znuB Zur OxyR anti-anti-terminator anti-terminator znuAp znuAp znuCp znuCp yebAp5 yebAp5 yebAp6 yebAp6 yebAp1 yebAp1 TSS_2210 TSS_2210 TSS_2209 TSS_2209

Gene      
Name: znuA    Texpresso search in the literature
Synonym(s): ECK1858, G7017, b1857, yebL, yzzP
Genome position(nucleotides): 1941651 <-- 1942583 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
49.09
External database links:  
ASAP:
ABE-0006191
ECHOBASE:
EB2543
ECOCYC:
G7017
ECOLIHUB:
znuA
OU-MICROARRAY:
b1857
REGULONDB:
b1857
STRING:
511145.b1857
M3D: znuA
COLOMBOS: znuA
PortEco: b1857


Product      
Name: Zn2+ ABC transporter periplasmic binding protein
Synonym(s): YebL, YzzP, ZnuA
Sequence: Get amino acid sequence Fasta Format
Cellular location: periplasmic space
Molecular weight: 33.777
Isoelectric point: 5.881
Motif(s):
 
Type Positions Sequence
119 -> 137 HGDDDDHDHAEKSDEDHHH
167 -> 198 PQSRAKLDANLKDFEAQLASTETQVGNELAPL
200 -> 308 GKGYFVFHDAYGYFEKQFGLTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMGTLDPLGTNIKLGKTSYSEFLSQLANQYASCLK
262 -> 262 A
116 -> 138 KSIHGDDDDHDHAEKSDEDHHHG

 

Classification:
Multifun Terms (GenProtEC)  
  4 - transport --> 4.3 - Primary Active Transporters --> 4.3.A - Pyrophosphate Bond (ATP; GTP; P2) Hydrolysis-driven Active Transporters --> 4.3.A.1 - The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases --> 4.3.A.1.p - ABC superfamily, periplasmic binding component
Gene Ontology Terms (GO)  
cellular_component GO:0042597 - periplasmic space
molecular_function GO:0046872 - metal ion binding
GO:0008270 - zinc ion binding
biological_process GO:0006811 - ion transport
GO:0007155 - cell adhesion
GO:0030001 - metal ion transport
GO:0006829 - zinc ion transport
Note(s): Note(s): ...[more].
External database links:  
DIP:
DIP-12949N
ECOCYC:
ZNUA-MONOMER
ECOLIWIKI:
b1857
INTERPRO:
IPR035520
INTERPRO:
IPR006128
INTERPRO:
IPR006127
MODBASE:
P39172
PDB:
2PRS
PDB:
2OGW
PDB:
2OSV
PDB:
2PS3
PDB:
2PS0
PDB:
2PS9
PFAM:
PF01297
PRIDE:
P39172
PRINTS:
PR00690
PRODB:
PRO_000024252
PROTEINMODELPORTAL:
P39172
REFSEQ:
NP_416371
SMR:
P39172
UNIPROT:
P39172


Operon      
Name: znuA         
Operon arrangement:
Transcription unit        Promoter
znuA


Transcriptional Regulation      
Display Regulation             
Activated by: OxyR
Repressed by: Zur
Growth Conditions:

[1] 

C: Escherichia coli| znuA knockout mutant| M9 minimal medium| (NH4)2Fe(SO4)2 30 然; glucose 0.2%; p-aminobenzoic acid 40 然; p-hydroxybenzoic acid 40 然; phenylalanine 0.1 mg/mL; tryptophan; tyrosine 0.1 mg/mL
E: Escherichia coli| znuA knockout mutant| M9 minimal medium| (NH4)2Fe(SO4)2 30 然; zinc sulfate 5 然; glucose 0.2%; p-aminobenzoic acid 40 然; p-hydroxybenzoic acid 40 然; phenylalanine 0.1 mg/mL; tryptophan; tyrosine 0.1 mg/mL

[2] 

C: Escherichia coli| znuA knockout mutant| MacConkey agar| (NH4)2Fe(SO4)2 30 然| 30.0 C
E: Escherichia coli| znuA knockout mutant| MacConkey agar| (NH4)2Fe(SO4)2 30 然; desferrioxamine B mesylate 20 mM| 30.0 C

[3] 

C: Escherichia coli| znuA knockout mutant| MacConkey agar| 30.0 C
E: Escherichia coli| znuA knockout mutant| MacConkey agar| (NH4)2Fe(SO4)2 10 mM| 30.0 C

[4] 

C: Escherichia coli| znuA knockout mutant| MacConkey agar| (NH4)2Fe(SO4)2 30 然| 30.0 C
E: Escherichia coli| znuA knockout mutant| MacConkey agar| (NH4)2Fe(SO4)2 30 然; zinc sulfate 1 mM| 30.0 C

[5] 

C: Escherichia coli| znuA knockout mutant| MacConkey agar| 30.0 C
E: Escherichia coli| znuA knockout mutant| MacConkey agar| EGTA 50 mM| 30.0 C

[6] 

C: Escherichia coli| znuA knockout mutant| MacConkey agar| 30.0 C
E: Escherichia coli| znuA knockout mutant| MacConkey agar| MnCl2 1 mM| 30.0 C

[7] 

C: Escherichia coli| znuA knockout mutant| M9 minimal medium| (NH4)2Fe(SO4)2 30 然; glucose 0.2%; p-aminobenzoic acid 40 然; p-hydroxybenzoic acid 40 然; phenylalanine 0.1 mg/mL; tryptophan; tyrosine 0.1 mg/mL
E: Escherichia coli| znuA knockout mutant| M9 minimal medium| desferrioxamine B mesylate 5 然; ferri-desferal 10 然; glucose 0.2%; p-aminobenzoic acid 40 然; p-hydroxybenzoic acid 40 然; phenylalanine 0.1 mg/mL; tryptophan; tyrosine 0.1 mg/mL



Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2209 1940588 reverse nd [RS-EPT-CBR] [1]
  promoter TSS_2210 1940853 reverse nd [RS-EPT-CBR] [1]
  promoter yebAp1 1941683 reverse Similarity to the consensus
Read more >
[ICWHO] [2]
  promoter yebAp6 1941721 reverse Similarity to the consensus
Read more >
[ICWHO] [2]
  promoter yebAp5 1941833 reverse Similarity to the consensus
Read more >
[ICWHO] [2]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Mu蕞iz-Rascado L, Garc蕞a-Sotelo JS, Weiss V, Solano-Lira H, Mart蕞nez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hern蕞ndez S, Alquicira-Hern蕞ndez K, L蕞pez-Fuentes A, Porr蕞n-Sotelo L, Huerta AM, Bonavides-Mart蕞nez C, Balderas-Mart蕞nez YI, Pannier L, Olvera M, Labastida A, Jim蕞nez-Jacinto V, Vega-Alvarado L, Del Moral-Ch蕞vez V, Hern蕞ndez-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


RegulonDB