RegulonDB

Gene
Name:	lpxP
Synonym(s):	ECK2374, G7241, b2378, ddg
Genome position(nucleotides):	2495645 --> 2496565 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.47
External database links:

ASAP:	ABE-0007848
ECHOBASE:	EB2738
OU-MICROARRAY:	b2378
PortEco:	lpxP
STRING:	511145.b2378
COLOMBOS:	lpxP

Product

Name:

palmitoleoyl acyltransferase

Synonym(s):

Ddg, LpxP

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

35.493

Isoelectric point:

10.277

Motif(s):

Type	Positions	Sequence
	20 -> 40	WFGLGVLWLWVQLPYPVLCFL
	5 -> 296	CKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRIGAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDSRVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQLMEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVENVATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKIIEKEIMRAPEQYLWIHRRFKT
	132 -> 137	HFMSLE

Classification:

Multifun Terms (GenProtEC)
		1 - metabolism --> 1.6 - biosynthesis of macromolecules (cellular constituents) --> 1.6.3 - lipopolysaccharide --> 1.6.3.3 - lipid A
		5 - cell processes --> 5.5 - adaptations --> 5.5.2 - temperature extremes
		6 - cell structure --> 6.1 - membrane
		6 - cell structure --> 6.3 - surface antigens (ECA, O antigen of LPS)
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	molecular_function	GO:0016740 - transferase activity GO:0016746 - transferase activity, transferring acyl groups GO:0008951 - palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity
	biological_process	GO:0009103 - lipopolysaccharide biosynthetic process GO:0009245 - lipid A biosynthetic process GO:0009409 - response to cold GO:0036104 - Kdo2-lipid A biosynthetic process

Note(s):

Note(s): ...[more].

Reference(s):

[1] Opiyo SO., et al., 2010

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

lpxP

Operon arrangement:

Transcription unit	Promoter
lpxP	lpxPp

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	yfdYp5	2495333	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 3.684. P-value: 3.947e-5 Read more >	[ICWHO]	[2]
promoter	yfdYp6	2495336	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 5.746. P-value: 1.605e-5 Read more >	[ICWHO]	[2]
promoter	TSS_2670	2495493	forward	nd	[RS-EPT-CBR]	[3]

Evidence
[ICWHO] Inferred computationally without human oversight
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Opiyo SO., Pardy RL., Moriyama H., Moriyama EN., 2010, Evolution of the Kdo2-lipid A biosynthesis in bacteria., BMC Evol Biol 10:362
[2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78
[3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.