RegulonDB RegulonDB 10.8: Gene Form
   

nrdI gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

nrdE nrdF nrdI ygaM nrdH NrdR NrdR Fur terminator anti-terminator anti-anti-terminator nrdHp nrdHp ygaCp ygaCp

Gene      
Name: nrdI    Texpresso search in the literature
Synonym(s): ECK2668, G7402, b2674, ygaO
Genome position(nucleotides): 2800965 --> 2801375 Genome Browser
Strand: forward
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
55.96
External database links:  
ASAP:
ABE-0008804
ECHOBASE:
EB3072
OU-MICROARRAY:
b2674
PortEco:
nrdI
STRING:
511145.b2674
COLOMBOS: nrdI


Product      
Name: dimanganese-tyrosyl radical cofactor maintenance flavodoxin NrdI
Synonym(s): NrdI, YgaO, flavodoxin NrdI, flavodoxin involved in dimanganese-tyrosyl radical cofactor maintenance for ribonucleotide reductase
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 15.34
Isoelectric point: 9.531
Motif(s):
 
Type Positions Sequence
5 -> 122 VYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYILIVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCGVPWLYRFELMGTQSDIENVR

 

Classification:
Multifun Terms (GenProtEC)  
  2 - information transfer --> 2.3 - protein related --> 2.3.3 - posttranslational modification
Gene Ontology Terms (GO)  
cellular_component GO:0005829 - cytosol
molecular_function GO:0005515 - protein binding
GO:0010181 - FMN binding
biological_process GO:0006464 - cellular protein modification process
Note(s): Note(s): ...[more].
Evidence: [APPH] Assay of protein purified to homogeneity
Reference(s): [1] Cotruvo JA., et al., 2008
[2] Cotruvo JA., et al., 2011
External database links:  
ECOCYC:
G7402-MONOMER
ECOLIWIKI:
b2674
INTERPRO:
IPR029039
INTERPRO:
IPR020852
INTERPRO:
IPR004465
MODBASE:
P0A772
PANTHER:
PTHR37297
PDB:
3N3A
PDB:
3N39
PDB:
3N3B
PFAM:
PF07972
PRIDE:
P0A772
PRODB:
PRO_000023409
REFSEQ:
NP_417160
SMR:
P0A772
UNIPROT:
P0A772


Operon      
Name: nrdHIEF         
Operon arrangement:
Transcription unit        Promoter
nrdHIEF


Transcriptional Regulation      
Display Regulation             
Activated by: IscR
Repressed by: Fur, NrdR


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References


Reference(s)    

 [1] Cotruvo JA., Stubbe J., 2008, NrdI, a flavodoxin involved in maintenance of the diferric-tyrosyl radical cofactor in Escherichia coli class Ib ribonucleotide reductase., Proc Natl Acad Sci U S A 105(38):14383-8

 [2] Cotruvo JA., Stubbe J., 2011, Escherichia coli Class Ib Ribonucleotide Reductase Contains a Dimanganese(III)-Tyrosyl Radical Cofactor in Vivo., Biochemistry 50(10):1672-81


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