RegulonDB

RegulonDB RegulonDB 11.0: Gene Form

cas1 gene in Escherichia coli K-12 genome

Gene local context to scale (view description)

Gene
Name:	cas1
Synonym(s):	ECK2750, G7425, b2755, ygbT
Genome position(nucleotides):	2878855 <-- 2879772
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	51.31
External database links:

ASAP:	ABE-0009038
ECHOBASE:	EB2915
ECOLIHUB:	cas1
OU-MICROARRAY:	b2755
STRING:	511145.b2755
COLOMBOS:	cas1

Product

Name:

multifunctional nuclease Cas1

Synonym(s):

Cas1, YgbT

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

33.194

Isoelectric point:

8.843

Motif(s):

Type	Positions	Sequence	Comment
	22 -> 22	Y	UniProt: Nearly wild-type spacer acquisition in vivo..
	41 -> 41	R	UniProt: Dramatically decreased spacer acquisition in vivo..
	59 -> 59	R	UniProt: Dramatically decreased spacer acquisition in vitro, 250-fold decreased affinity for protospacer DNA..
	66 -> 66	R	UniProt: Dramatically decreased spacer acquisition in vitro, 250-fold decreased affinity for protospacer DNA..
	84 -> 84	R	UniProt: Dramatically decreased spacer acquisition in vivo..

Type	Positions	Sequence	Comment
	22 -> 22	Y	UniProt: Nearly wild-type spacer acquisition in vivo..
	41 -> 41	R	UniProt: Dramatically decreased spacer acquisition in vivo..
	59 -> 59	R	UniProt: Dramatically decreased spacer acquisition in vitro, 250-fold decreased affinity for protospacer DNA..
	66 -> 66	R	UniProt: Dramatically decreased spacer acquisition in vitro, 250-fold decreased affinity for protospacer DNA..
	84 -> 84	R	UniProt: Dramatically decreased spacer acquisition in vivo..
	96 -> 278	SDKLLYQAKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYALLAKQYGVTWNGRRYDPKDWEKGDTINQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYDIADIIKFDTVVPKAFEIARRNPGEPDREVRLACRDIFRSSKTLAKLIPLIEDVLAAGEIQ	UniProt: Sufficient for cleavage of ssRNA, ssDNA and Holliday junction DNA; Sequence Annotation Type: region of interest.
	130 -> 228	ARRSVEQLRGIEGSRVRATYALLAKQYGVTWNGRRYDPKDWEKGDTINQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYDIADIIKFDTV
	141 -> 141	E	E141A abolished nuclease activity \|CITS: [21219465]\|.
	149 -> 149	Y	UniProt: No effect on in vitro protospacer integration..
	165 -> 165	Y	UniProt: No effect on in vitro protospacer integration (PubMed:25707795). Alone significantly decreased protospacer acquisition in vivo (PubMed:26478180). Loss of protospacer acquisition, decreased protospacer binding; in association with A-170, significantly decreased protospacer binding; in association with A-217 (PubMed:26478180)..
	170 -> 170	W	UniProt: Alone significantly decreased protospacer acquisition in vivo (PubMed:26478180). Decreased protospacer binding; in association with A-170 (PubMed:26478180)..
	184 -> 184	T	UniProt: No cleavage of any substrates..
	188 -> 188	Y	UniProt: Partial inhibition of cleavage (PubMed:21219465). No effect on in vitro protospacer integration (PubMed:25707795). Significantly decreased protospacer acquisition in vivo (PubMed:26478180)..
	208 -> 208	H	H208A abolished nuclease activity \|CITS: [21219465]\|.
	211 -> 211	K	UniProt: No cleavage of any substrates..
	217 -> 217	Y	UniProt: No effect on in vitro protospacer integration (PubMed:25707795). Alone significantly decreased protospacer acquisition in vivo (PubMed:26478180). Significantly decreased protospacer binding; in association with A-165 (PubMed:26478180)..
	218 -> 218	D	UniProt: No cleavage of any substrates, no restoration of UV or MMC resistance (PubMed:21219465). Loss of spacer acquisition in vivo (PubMed:24793649)..
	221 -> 221	D	D221A abolished nuclease activity \|CITS: [21219465]\|.
	224 -> 224	K	UniProt: No cleavage of any substrates (PubMed:21219465). Loss of spacer acquisition in vivo (PubMed:24793649, PubMed:25707795)..
	245 -> 245	R	UniProt: No effect on spacer acquisition..
	248 -> 248	R	UniProt: Dramatically decreased spacer acquisition in vivo..
	252 -> 252	R	UniProt: No effect on spacer acquisition..
	256 -> 256	R	UniProt: No effect on spacer acquisition..
	278 -> 305	QPPAPPEDAQPVAIPLPVSLGDAGHRSS	UniProt: Disordered; Sequence Annotation Type: region of interest.
	282 -> 305	PPEDAQPVAIPLPVSLGDAGHRSS	UniProt: No effect on spacer acquisition, Cas1-Cas2 complex formation or CRISPR DNA-binding by complex..
	291 -> 291	I	UniProt: No effect on spacer acquisition..

Classification:

Multifun Terms (GenProtEC)
		2 - information transfer --> 2.1 - DNA related --> 2.1.4 - DNA repair
		5 - cell processes --> 5.10 - defense/survival
Gene Ontology Terms (GO)
	cellular_component	GO:0005737 - cytoplasm GO:0005829 - cytosol
	molecular_function	GO:0003676 - nucleic acid binding GO:0003677 - DNA binding GO:0004518 - nuclease activity GO:0004519 - endonuclease activity GO:0005515 - protein binding GO:0016787 - hydrolase activity GO:0046872 - metal ion binding GO:0004520 - endodeoxyribonuclease activity GO:0008821 - crossover junction endodeoxyribonuclease activity GO:0042802 - identical protein binding GO:0042803 - protein homodimerization activity GO:0017108 - 5'-flap endonuclease activity
	biological_process	GO:0099048 - CRISPR-cas system GO:0006281 - DNA repair GO:0006974 - cellular response to DNA damage stimulus GO:0051607 - defense response to virus GO:0090305 - nucleic acid phosphodiester bond hydrolysis GO:0043571 - maintenance of CRISPR repeat elements

External database links:

ALPHAFOLD:

DIP:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

PDB:

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REFSEQ:

SMR:

UNIPROT:

Operon

Name:

casABCDE12

Operon arrangement:

Transcription unit	Promoter
ygbF	ygbFp
casABCDE12	casAp
casDE-ygbTF	casDp

Transcriptional Regulation
	Display Regulation
Activated by:	LeuO, SlyA, StpA
Repressed by:	CRP, H-NS

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

RegulonDB

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