RegulonDB RegulonDB 10.8: Gene Form
   

casA gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

cas3 casA casC casB LeuO CRP LeuO H-NS LeuO LeuO SlyA TSS_3043 TSS_3043 casAp casAp

Gene      
Name: casA    Texpresso search in the literature
Synonym(s): ECK2755, G7430, b2760, cse1, ygcL
Genome position(nucleotides): 2882630 <-- 2884138 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
43.8
External database links:  
ASAP:
ABE-0009050
ECHOBASE:
EB2920
OU-MICROARRAY:
b2760
PortEco:
casA
STRING:
511145.b2760
COLOMBOS: casA


Product      
Name: type I-E CRISPR system Cascade subunit CasA
Synonym(s): CasA, Cascade subunit A, Cse1, YgcL
Sequence: Get amino acid sequence Fasta Format
Molecular weight: 55.901
Isoelectric point: 8.346
Motif(s):
 
Type Positions Sequence
130 -> 130 V
288 -> 294 VKKGEVE
129 -> 129 F
131 -> 131 N
318 -> 323 IIQNEN

 

Classification:
Multifun Terms (GenProtEC)  
  5 - cell processes --> 5.10 - defense/survival
Gene Ontology Terms (GO)  
cellular_component GO:0032991 - protein-containing complex
molecular_function GO:0003677 - DNA binding
GO:0005515 - protein binding
GO:0003723 - RNA binding
GO:0008270 - zinc ion binding
biological_process GO:0051607 - defense response to virus
Note(s): Note(s): ...[more].
Reference(s): [1] Hochstrasser ML., et al., 2014
[2] Sashital DG., et al., 2012
External database links:  
DIP:
DIP-12128N
ECOCYC:
G7430-MONOMER
ECOLIWIKI:
b2760
INTERPRO:
IPR013381
PDB:
5H9F
PDB:
5H9E
PDB:
4U7U
PDB:
4TVX
PDB:
4QYZ
PDB:
5CD4
PFAM:
PF09481
PRIDE:
Q46901
REFSEQ:
NP_417240
SMR:
Q46901
UNIPROT:
Q46901


Operon      
Name: casABCDE12         
Operon arrangement:
Transcription unit        Promoter
ygbF
casABCDE12
casDE-ygbTF


Transcriptional Regulation      
Display Regulation             
Activated by: LeuO, SlyA
Repressed by: CRP, H-NS
Growth Conditions:

[1] 

C: Escherichia coli| plasmid pBAD/Myc-HisA mutant| LB medium| 37.0 C| mid exponential phase
E: Escherichia coli| plasmid pBADBaeR (arabinose-induced baeR) mutant| LB medium| 37.0 C| mid exponential phase



Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3043 2884659 reverse nd [RS-EPT-CBR] [3]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates



Reference(s)    

 [1] Hochstrasser ML., Taylor DW., Bhat P., Guegler CK., Sternberg SH., Nogales E., Doudna JA., 2014, CasA mediates Cas3-catalyzed target degradation during CRISPR RNA-guided interference., Proc Natl Acad Sci U S A 111(18):6618-23

 [2] Sashital DG., Wiedenheft B., Doudna JA., 2012, Mechanism of foreign DNA selection in a bacterial adaptive immune system., Mol Cell 46(5):606-15

 [3] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.


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