RegulonDB

Gene
Name:	glcD
Synonym(s):	ECK2974, G7545, b2979, gox, yghM
Genome position(nucleotides):	3126522 <-- 3128021 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	56.67
External database links:

ASAP:	ABE-0009773
ECHOBASE:	EB2820
ECOLIHUB:	glcD
OU-MICROARRAY:	b2979
STRING:	511145.b2979
COLOMBOS:	glcD

Shine dalgarno
Sequence:	cacaacaaagCGAAGCctactCAT

Product

Name:

glycolate dehydrogenase, putative FAD-linked subunit

Synonym(s):

GlcD, Gox, YghM

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane,cytosol

Molecular weight:

53.812

Isoelectric point:

4.825

Motif(s):

Type	Positions	Sequence
	56 -> 194	PLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTL
	231 -> 470	PPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGK
	52 -> 230	YRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPK

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.25 - glycolate metabolism
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol GO:0016020 - membrane GO:0005886 - plasma membrane GO:0009339 - glycolate oxidase complex
	molecular_function	GO:0003824 - catalytic activity GO:0016491 - oxidoreductase activity GO:0019154 - glycolate dehydrogenase activity GO:0050660 - flavin adenine dinucleotide binding GO:0008891 - glycolate oxidase activity GO:0071949 - FAD binding
	biological_process	GO:0006974 - cellular response to DNA damage stimulus GO:0046296 - glycolate catabolic process

Note(s):

Note(s): ...[more].

Reference(s):

[1] Cabulong RB., et al., 2018
[2] Choi SY., et al., 2016
[3] Matsumoto K., et al., 2019
[4] Si HM., et al., 2016
[5] Sweeney J., et al., 2015

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

MODBASE:

PFAM:

PFAM:

PRIDE:

PROSITE:

REFSEQ:

UNIPROT:

Operon

Name:

glcDEFGBA

Operon arrangement:

Transcription unit	Promoter
glcDEFGBA	glcDp
glcBA	glcBp

Transcriptional Regulation
	Display Regulation
Activated by:	GlcC, IHF
Repressed by:	ArcA, PdhR

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	TSS_3300	3124265		reverse	nd	[RS-EPT-CBR]	[6]

Evidence
[RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

Reference(s)
[1] Cabulong RB., Lee WK., Banares AB., Ramos KRM., Nisola GM., Valdehuesa KNG., Chung WJ., 2018, Engineering Escherichia coli for glycolic acid production from D-xylose through the Dahms pathway and glyoxylate bypass., Appl Microbiol Biotechnol 102(5):2179-2189
[2] Choi SY., Park SJ., Kim WJ., Yang JE., Lee H., Shin J., Lee SY., 2016, One-step fermentative production of poly(lactate-co-glycolate) from carbohydrates in Escherichia coli., Nat Biotechnol 34(4):435-40
[3] Matsumoto K., Saito J., Yokoo T., Hori C., Nagata A., Kudoh Y., Ooi T., Taguchi S., 2019, Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli., J Biosci Bioeng 128(3):302-306
[4] Si HM., Zhang F., Wu AN., Han RZ., Xu GC., Ni Y., 2016, DNA microarray of global transcription factor mutant reveals membrane-related proteins involved in n-butanol tolerance in Escherichia coli., Biotechnol Biofuels 9:114
[5] Sweeney J., Murphy CD., McDonnell K., 2015, Towards an effective biosensor for monitoring AD leachate: a knockout E. coli mutant that cannot catabolise lactate., Appl Microbiol Biotechnol 99(23):10209-14
[6] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.