RegulonDB

Gene
Name:	mqsR
Synonym(s):	ECK3013, G7572, b3022, ygiU
Genome position(nucleotides):	3168248 <-- 3168544 Genome Browser
Strand:	reverse
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	40.07
External database links:

ASAP:	ABE-0009925
ECHOBASE:	EB2841
ECOLIHUB:	mqsR
OU-MICROARRAY:	b3022
STRING:	511145.b3022
COLOMBOS:	mqsR

Product

Name:

mRNA interferase toxin MqsR

Synonym(s):

MqsR, YgiU, mRNA interferase / toxin of the MqsR-MqsA toxin-antitoxin system

Sequence:

Get amino acid sequence Fasta Format

Molecular weight:

11.232

Isoelectric point:

9.077

Motif(s):

Type	Positions	Sequence
	68 -> 68	Q
	56 -> 56	K
	96 -> 96	K
	81 -> 81	Y
	58 -> 58	M

Classification:

Multifun Terms (GenProtEC)
		3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.1 - translation attenuation and efficiency
		5 - cell processes --> 5.3 - motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)
Gene Ontology Terms (GO)
	molecular_function	GO:0004518 - nuclease activity GO:0004519 - endonuclease activity GO:0005515 - protein binding GO:0016787 - hydrolase activity GO:0004521 - endoribonuclease activity GO:0016892 - endoribonuclease activity, producing 3'-phosphomonoesters
	biological_process	GO:0061157 - mRNA destabilization GO:0009372 - quorum sensing GO:0045892 - negative regulation of transcription, DNA-templated GO:0017148 - negative regulation of translation GO:0043488 - regulation of mRNA stability GO:0044010 - single-species biofilm formation GO:0090305 - nucleic acid phosphodiester bond hydrolysis GO:2000145 - regulation of cell motility GO:0090502 - RNA phosphodiester bond hydrolysis, endonucleolytic

Note(s):

Note(s): ...[more].

Evidence:

[IDA] Inferred from direct assay

Reference(s):

[1] Cheng HY., et al., 2014
[2] Chowdhury N., et al., 2016
[3] Figueira R., et al., 2015
[4] Fraikin N., et al., 2019
[5] Liu S., et al., 2017
[6] Tsukuda M., et al., 2015
[7] Wu N., et al., 2015
[8] Yamaguchi Y., et al., 2009

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

PDB:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

mqsRA

Operon arrangement:

Transcription unit	Promoter
mqsA	mqsAp1
mqsA	mqsAp2
mqsRA	mqsRp2
mqsRA	mqsRp1

Transcriptional Regulation
	Display Regulation
Repressed by:	MqsA

RNA cis-regulatory element

Attenuation:	Transcriptional

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
	promoter	mqsAp9	3168371		reverse	nd	[ICWHO]	[9]

Evidence
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Cheng HY., Soo VW., Islam S., McAnulty MJ., Benedik MJ., Wood TK., 2014, Toxin GhoT of the GhoT/GhoS toxin/antitoxin system damages the cell membrane to reduce adenosine triphosphate and to reduce growth under stress., Environ Microbiol 16(6):1741-54
[2] Chowdhury N., Kwan BW., Wood TK., 2016, Persistence Increases in the Absence of the Alarmone Guanosine Tetraphosphate by Reducing Cell Growth., Sci Rep 6:20519
[3] Figueira R., Brown DR., Ferreira D., Eldridge MJ., Burchell L., Pan Z., Helaine S., Wigneshweraraj S., 2015, Adaptation to sustained nitrogen starvation by Escherichia coli requires the eukaryote-like serine/threonine kinase YeaG., Sci Rep 5:17524
[4] Fraikin N., Rousseau CJ., Goeders N., Van Melderen L., 2019, Reassessing the Role of the Type II MqsRA Toxin-Antitoxin System in Stress Response and Biofilm Formation: mqsA Is Transcriptionally Uncoupled from mqsR., mBio 10(6)
[5] Liu S., Wu N., Zhang S., Yuan Y., Zhang W., Zhang Y., 2017, Variable Persister Gene Interactions with (p)ppGpp for Persister Formation in Escherichia coli., Front Microbiol 8:1795
[6] Tsukuda M., Nakashima N., Miyazaki K., 2015, Counterselection method based on conditional silencing of antitoxin genes in Escherichia coli., J Biosci Bioeng 120(5):591-5
[7] Wu N., He L., Cui P., Wang W., Yuan Y., Liu S., Xu T., Zhang S., Wu J., Zhang W., Zhang Y., 2015, Ranking of persister genes in the same Escherichia coli genetic background demonstrates varying importance of individual persister genes in tolerance to different antibiotics., Front Microbiol 6:1003
[8] Yamaguchi Y., Park JH., Inouye M., 2009, MqsR, a crucial regulator for quorum sensing and biofilm formation, is a GCU-specific mRNA interferase in Escherichia coli., J Biol Chem 284(42):28746-53
[9] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78