RegulonDB RegulonDB 10.10: Gene Form
   

mqsR gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

ygiV mqsA mqsR MqsA MqsA anti-anti-terminator anti-terminator terminator anti-terminator mqsRp mqsRp mqsRp2 mqsRp2 mqsAp2 mqsAp2 mqsAp9 mqsAp9 mqsAp1 mqsAp1

Gene      
Name: mqsR    Texpresso search in the literature
Synonym(s): ECK3013, G7572, b3022, ygiU
Genome position(nucleotides): 3168248 <-- 3168544 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
40.07
External database links:  
ASAP:
ABE-0009925
ECHOBASE:
EB2841
ECOLIHUB:
mqsR
OU-MICROARRAY:
b3022
STRING:
511145.b3022
COLOMBOS: mqsR


Product      
Name: mRNA interferase toxin MqsR
Synonym(s): MqsR, YgiU, mRNA interferase / toxin of the MqsR-MqsA toxin-antitoxin system
Sequence: Get amino acid sequence Fasta Format
Molecular weight: 11.232
Isoelectric point: 9.077
Motif(s):
 
Type Positions Sequence
68 -> 68 Q
56 -> 56 K
96 -> 96 K
81 -> 81 Y
58 -> 58 M

 

Classification:
Multifun Terms (GenProtEC)  
  3 - regulation --> 3.1 - type of regulation --> 3.1.3 - posttranscriptional --> 3.1.3.1 - translation attenuation and efficiency
  5 - cell processes --> 5.3 - motility, chemotaxis, energytaxis (aerotaxis, redoxtaxis etc)
Gene Ontology Terms (GO)  
molecular_function GO:0004518 - nuclease activity
GO:0004519 - endonuclease activity
GO:0005515 - protein binding
GO:0016787 - hydrolase activity
GO:0004521 - endoribonuclease activity
GO:0016892 - endoribonuclease activity, producing 3'-phosphomonoesters
biological_process GO:0061157 - mRNA destabilization
GO:0009372 - quorum sensing
GO:0045892 - negative regulation of transcription, DNA-templated
GO:0017148 - negative regulation of translation
GO:0043488 - regulation of mRNA stability
GO:0044010 - single-species biofilm formation
GO:0090305 - nucleic acid phosphodiester bond hydrolysis
GO:2000145 - regulation of cell motility
GO:0090502 - RNA phosphodiester bond hydrolysis, endonucleolytic
Note(s): Note(s): ...[more].
Evidence: [IDA] Inferred from direct assay
Reference(s): [1] Cheng HY., et al., 2014
[2] Chowdhury N., et al., 2016
[3] Figueira R., et al., 2015
[4] Fraikin N., et al., 2019
[5] Liu S., et al., 2017
[6] Tsukuda M., et al., 2015
[7] Wu N., et al., 2015
[8] Yamaguchi Y., et al., 2009
External database links:  
ECOCYC:
G7572-MONOMER
ECOLIWIKI:
b3022
INTERPRO:
IPR038493
INTERPRO:
IPR031451
PDB:
3HI2
PFAM:
PF15723
PRIDE:
Q46865
PRODB:
PRO_000023281
REFSEQ:
NP_417494
SMR:
Q46865
UNIPROT:
Q46865


Operon      
Name: mqsRA         
Operon arrangement:
Transcription unit        Promoter
mqsA
mqsA
mqsRA
mqsRA


Transcriptional Regulation      
Display Regulation             
Repressed by: MqsA


RNA cis-regulatory element    
Attenuation: Transcriptional


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter mqsAp9 3168371 reverse nd [ICWHO] [9]


Evidence    

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Cheng HY., Soo VW., Islam S., McAnulty MJ., Benedik MJ., Wood TK., 2014, Toxin GhoT of the GhoT/GhoS toxin/antitoxin system damages the cell membrane to reduce adenosine triphosphate and to reduce growth under stress., Environ Microbiol 16(6):1741-54

 [2] Chowdhury N., Kwan BW., Wood TK., 2016, Persistence Increases in the Absence of the Alarmone Guanosine Tetraphosphate by Reducing Cell Growth., Sci Rep 6:20519

 [3] Figueira R., Brown DR., Ferreira D., Eldridge MJ., Burchell L., Pan Z., Helaine S., Wigneshweraraj S., 2015, Adaptation to sustained nitrogen starvation by Escherichia coli requires the eukaryote-like serine/threonine kinase YeaG., Sci Rep 5:17524

 [4] Fraikin N., Rousseau CJ., Goeders N., Van Melderen L., 2019, Reassessing the Role of the Type II MqsRA Toxin-Antitoxin System in Stress Response and Biofilm Formation: mqsA Is Transcriptionally Uncoupled from mqsR., mBio 10(6)

 [5] Liu S., Wu N., Zhang S., Yuan Y., Zhang W., Zhang Y., 2017, Variable Persister Gene Interactions with (p)ppGpp for Persister Formation in Escherichia coli., Front Microbiol 8:1795

 [6] Tsukuda M., Nakashima N., Miyazaki K., 2015, Counterselection method based on conditional silencing of antitoxin genes in Escherichia coli., J Biosci Bioeng 120(5):591-5

 [7] Wu N., He L., Cui P., Wang W., Yuan Y., Liu S., Xu T., Zhang S., Wu J., Zhang W., Zhang Y., 2015, Ranking of persister genes in the same Escherichia coli genetic background demonstrates varying importance of individual persister genes in tolerance to different antibiotics., Front Microbiol 6:1003

 [8] Yamaguchi Y., Park JH., Inouye M., 2009, MqsR, a crucial regulator for quorum sensing and biofilm formation, is a GCU-specific mRNA interferase in Escherichia coli., J Biol Chem 284(42):28746-53

 [9] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


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