RegulonDB RegulonDB 10.10: Gene Form
   

obgE gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

dacB obgE yhbE TSS_3491 (cluster) TSS_3491 (cluster)

Gene      
Name: obgE    Texpresso search in the literature
Synonym(s): ECK3172, G7656, b3183, cgtA, obg, yhbZ
Genome position(nucleotides): 3330582 <-- 3331754 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
 53.2
External database links:  
ASAP:
 ABE-0010462
ECHOBASE:
 EB2647
ECOLIHUB:
 obgE
OU-MICROARRAY:
 b3183
STRING:
 511145.b3183
COLOMBOS: obgE


Product      
Name: GTPase ObgE
Synonym(s): CgtA, GTPase involved in chromosome partitioning and ribosome assembly, Obg, ObgE, YhbZ
Sequence: Get amino acid sequence Fasta Format
Cellular location: cytosol
Molecular weight: 43.286
Isoelectric point: 4.495
Motif(s):
 
Type Positions Sequence
136 -> 139 RTPR
166 -> 173 GMPNAGKS
213 -> 216 DIPG
283 -> 286 NKID
76 -> 82 RDCTGKR

 
Classification:
Multifun Terms (GenProtEC)  
  3 - regulation --> 3.1 - type of regulation --> 3.1.4 - unknown
  5 - cell processes --> 5.1 - cell division
  5 - cell processes --> 5.2 - cell cycle physiology
Gene Ontology Terms (GO)  
cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0003677 - DNA binding
GO:0005515 - protein binding
GO:0016787 - hydrolase activity
GO:0046872 - metal ion binding
GO:0003723 - RNA binding
GO:0000166 - nucleotide binding
GO:0003924 - GTPase activity
GO:0019843 - rRNA binding
GO:0005525 - GTP binding
GO:0032561 - guanyl ribonucleotide binding
GO:0000287 - magnesium ion binding
GO:0019003 - GDP binding
GO:0043023 - ribosomal large subunit binding
GO:0097216 - guanosine tetraphosphate binding
biological_process GO:0042254 - ribosome biogenesis
GO:0042255 - ribosome assembly
GO:0007059 - chromosome segregation
GO:0022611 - dormancy process
GO:0090071 - negative regulation of ribosome biogenesis
Note(s): Note(s): ...[more].
Evidence: [IDA] Inferred from direct assay
Reference(s): [1] Chen SS., et al., 2013
[2] Feng B., et al., 2014
[3] Wang X., et al., 2012
External database links:  
DIP:
 DIP-12273N
ECOCYC:
 G7656-MONOMER
ECOLIWIKI:
 b3183
INTERPRO:
 IPR036726
INTERPRO:
 IPR035101
INTERPRO:
 IPR031167
INTERPRO:
 IPR006073
INTERPRO:
 IPR006074
INTERPRO:
 IPR006169
INTERPRO:
 IPR014100
INTERPRO:
 IPR027417
MODBASE:
 P42641
PDB:
 5M04
PDB:
 4CSU
PFAM:
 PF01018
PFAM:
 PF01926
PRIDE:
 P42641
PRINTS:
 PR00326
PRODB:
 PRO_000023448
PROSITE:
 PS51883
PROSITE:
 PS00905
PROSITE:
 PS51710
REFSEQ:
 NP_417650
SMR:
 P42641
SWISSMODEL:
 P42641
UNIPROT:
 P42641


Operon      
Name: rplU-rpmA-yhbE-obgE         
Operon arrangement:
Transcription unit        Promoter
rplU-rpmA
rplU-rpmA-yhbE-obgE
yhbE-obgE


Transcriptional Regulation      
Display Regulation             
Activated by: MlrA


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_3491 (cluster) 3330883 reverse nd [RS-EPT-CBR] [4]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates


Reference(s)    

 [1] Chen SS., Williamson JR., 2013, Characterization of the ribosome biogenesis landscape in E. coli using quantitative mass spectrometry., J Mol Biol 425(4):767-79

 [2] Feng B., Mandava CS., Guo Q., Wang J., Cao W., Li N., Zhang Y., Wang Z., Wu J., Sanyal S., Lei J., Gao N., 2014, Structural and functional insights into the mode of action of a universally conserved Obg GTPase., PLoS Biol 12(5):e1001866

 [3] Wang X., Xue J., Sun Z., Qin Y., Gong W., 2012, Study on the chaperone properties of conserved GTPases., Protein Cell 3(1):44-50

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

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