RegulonDB

Gene
Name:	kptA
Synonym(s):	ECK4322, G7928, b4331, yjiI
Genome position(nucleotides):	4560930 --> 4561484 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	50.81
External database links:

ASAP:	ABE-0014204
ECHOBASE:	EB2458
OU-MICROARRAY:	b4331
PortEco:	kptA
STRING:	511145.b4331
COLOMBOS:	kptA

Product

Name:

RNA 2'-phosphotransferase

Synonym(s):

KptA, YjiI

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

cytosol

Molecular weight:

20.53

Isoelectric point:

9.882

Motif(s):

Type	Positions	Sequence
	11 -> 175	DTSKFLSFVLRHKPEAIGIVLDREGWADIDKLILCAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKRGLPFWQAENGVWLTS

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		1 - metabolism --> 1.7 - central intermediary metabolism --> 1.7.1 - unassigned reversible reactions
Gene Ontology Terms (GO)
	cellular_component	GO:0005829 - cytosol
	molecular_function	GO:0016740 - transferase activity GO:0016772 - transferase activity, transferring phosphorus-containing groups GO:0016773 - phosphotransferase activity, alcohol group as acceptor
	biological_process	GO:0006388 - tRNA splicing, via endonucleolytic cleavage and ligation

Note(s):

Note(s): ...[more].

Evidence:

[IDA] Inferred from direct assay

Reference(s):

[1] Spinelli SL., et al., 1999
[2] Steiger MA., et al., 2001

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

INTERPRO:

INTERPRO:

INTERPRO:

PANTHER:

PFAM:

PRIDE:

PRODB:

REFSEQ:

SMR:

UNIPROT:

Operon

Name:

kptA

Operon arrangement:

Transcription unit	Promoter
kptA

Elements in the selected gene context region unrelated to any object in RegulonDB

	Type	Name	Post Left	Post Right	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References

Reference(s)
[1] Spinelli SL., Kierzek R., Turner DH., Phizicky EM., 1999, Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast., J Biol Chem 274(5):2637-44
[2] Steiger MA., Kierzek R., Turner DH., Phizicky EM., 2001, Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog., Biochemistry 40(46):14098-105