RegulonDB

Gene
Name:	yhiM
Synonym(s):	ECK3476, EG12228, b3491
Genome position(nucleotides):	3634841 --> 3635893 Genome Browser
Strand:	forward
Sequence:	Get nucleotide sequence FastaFormat
GC content %:	47.1
External database links:

ASAP:	ABE-0011400
ECHOBASE:	EB2140
PortEco:	yhiM
STRING:	511145.b3491
COLOMBOS:	yhiM

Product

Name:

inner membrane protein YhiM

Synonym(s):

YhiM, inner membrane protein with a role in acid resistance

Sequence:

Get amino acid sequence Fasta Format

Cellular location:

inner membrane

Molecular weight:

37.657

Isoelectric point:

7.966

Motif(s):

Type	Positions	Sequence
	42 -> 62	VLISLAAICLALFTTAFIIIS
	158 -> 178	CLIAVPVLLTLLGFIWSITLL
	291 -> 311	IPMIPVFTCLFCLFLASFLAE
	1 -> 347	MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLISLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMADEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLIAVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTFSTKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCFTLFSIVSILEAGS
	75 -> 95	LFPIIGYAGSIITMIWGWALL

Classification:

Multifun Terms (GenProtEC)
Multifun Terms (GenProtEC)		5 - cell processes --> 5.5 - adaptations --> 5.5.4 - pH
Gene Ontology Terms (GO)
	cellular_component	GO:0016020 - membrane GO:0005886 - plasma membrane GO:0005887 - integral component of plasma membrane GO:0016021 - integral component of membrane
	biological_process	GO:0071468 - cellular response to acidic pH

Note(s):

Note(s): ...[more].

Evidence:

[IEP] Inferred from expression pattern
[IMP] Inferred from mutant phenotype

Reference(s):

[1] Altuvia S., et al., 1997
[2] Nguyen TM., et al., 2012
[3] Tucker DL., et al., 2002

External database links:

ECOCYC:

ECOLIWIKI:

INTERPRO:

PFAM:

PRIDE:

REFSEQ:

UNIPROT:

Operon

Name:

yhiM

Operon arrangement:

Transcription unit	Promoter
yhiM	yhiMp

Transcriptional Regulation
	Display Regulation
Activated by:	GadE-RcsB, GadE
Repressed by:	H-NS

Regulation by small RNA
	Display Regulation
small RNA	oxyS

Elements in the selected gene context region unrelated to any object in RegulonDB

Type	Name	Post Left	Strand	Notes	Evidence (Confirmed, Strong, Weak)	References
promoter	yhiJp6	3632595	reverse	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 4.01. P-value: 6.979e-5 Read more >	[ICWHO]	[4]
promoter	yhiMp10	3634635	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: good homology. Score: 6.806. P-value: 7.811e-6 Read more >	[ICWHO]	[4]
promoter	yhiMp9	3634783	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 4.768. P-value: 3.199e-5 Read more >	[ICWHO]	[4]
promoter	yhiMp11	3634817	forward	Similarity to the consensus sequence of the set of known functional promoters for this sigma factor with strong experimental evidence: high homology. Score: 7.615. P-value: 1.381e-5 Read more >	[ICWHO]	[4]

Evidence
[ICWHO] Inferred computationally without human oversight

Reference(s)
[1] Altuvia S., Weinstein-Fischer D., Zhang A., Postow L., Storz G., 1997, A small, stable RNA induced by oxidative stress: role as a pleiotropic regulator and antimutator., Cell 90(1):43-53
[2] Nguyen TM., Sparks-Thissen RL., 2012, The inner membrane protein, YhiM, is necessary for Escherichia coli (E. coli) survival in acidic conditions., Arch Microbiol 194(7):637-41
[3] Tucker DL., Tucker N., Conway T., 2002, Gene expression profiling of the pH response in Escherichia coli., J Bacteriol 184(23):6551-8
[4] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78