RegulonDB RegulonDB 10.8: Gene Form
   

micL gene in Escherichia coli K-12 genome


Gene local context to scale (view description)

torY cutC yecM micL cutCp2 cutCp2 micLp2 micLp2 micLp micLp TSS_2221 TSS_2221

Gene      
Name: micL    Texpresso search in the literature
Synonym(s): ECK4574, G0-16601, b4717, ryeF, slrA
Genome position(nucleotides): 1958441 <-- 1958520 Genome Browser
Strand: reverse
Sequence: Get nucleotide sequence FastaFormat
GC content %:  
46.25
Reference(s): [1] Klein G., et al., 2014


Product      
Name: MicL
Synonym(s): MicL-S, RyeF, SlrA, ig:1179, ryeF, sirA, small regulatory RNA MicL-S
Type: small RNA
Classification:
Multifun Terms (GenProtEC)  
Gene Ontology Terms (GO)  
molecular_function GO:0005515 - protein binding
GO:0003729 - mRNA binding
biological_process GO:0040033 - negative regulation of translation, ncRNA-mediated
GO:1902201 - negative regulation of bacterial-type flagellum-dependent cell motility
Note(s): Note(s): ...[more].
Evidence: [IE] Inferred from experiment
[IGI] Inferred from genetic interaction
[IMP] Inferred from mutant phenotype
Reference(s): [2] Guo MS., et al., 2014
[1] Klein G., et al., 2014
[3] Nicoloff H., et al., 2017
[4] Zhang A., et al., 2003
External database links:  
ECOCYC:
RNA0-386


Operon      
Name: micL         
Operon arrangement:
Transcription unit        Promoter
micL
micL


Elements in the selected gene context region unrelated to any object in RegulonDB      

  Type Name Post Left Post Right Strand Notes Evidence (Confirmed, Strong, Weak) References
  promoter TSS_2221 1958648 reverse nd [RS-EPT-CBR] [5]
  promoter cutCp2 1959267 reverse Similarity to the consensus
Read more >
[ICWHO] [6]


Evidence    

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [ICWHO] Inferred computationally without human oversight



Reference(s)    

 [1] Klein G., Kobylak N., Lindner B., Stupak A., Raina S., 2014, Assembly of lipopolysaccharide in Escherichia coli requires the essential LapB heat shock protein., J Biol Chem 289(21):14829-53

 [2] Guo MS., Updegrove TB., Gogol EB., Shabalina SA., Gross CA., Storz G., 2014, MicL, a new σE-dependent sRNA, combats envelope stress by repressing synthesis of Lpp, the major outer membrane lipoprotein., Genes Dev 28(14):1620-34

 [3] Nicoloff H., Gopalkrishnan S., Ades SE., 2017, Appropriate Regulation of the σE-Dependent Envelope Stress Response Is Necessary To Maintain Cell Envelope Integrity and Stationary-Phase Survival in Escherichia coli, J Bacteriol 199(12)

 [4] Zhang A., Wassarman KM., Rosenow C., Tjaden BC., Storz G., Gottesman S., 2003, Global analysis of small RNA and mRNA targets of Hfq., Mol Microbiol 50(4):1111-24

 [5] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [6] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78


RegulonDB