RegulonDB RegulonDB

LsrR Genetic Sensory Response Unit in Escherichia coli K-12 genome


Gensor Unit Map          
(R)-THMF_Ext LsrA acetyl-CoA LsrC LsrR adenosylhomocysteine LsrD LsrB (4S)-4-hydroxy-2,3-pentanedione 5-phosphate LsrC LsrK (R)-THMF monomethyl-ester-of-trans-aconitate (4S)-4-hydroxy-5-phosphooxypentane-2,3-dione LsrA coenzyme A lsrRK lsrACDBFG-tam_mRNA LsrR LsrG DHAP 3-hydroxy-2,4-pentanedione phosphate (4S)-4-hydroxy-5-phosphooxypentane-2,3-dione SAM LsrD lsrACDBFG-tam LsrR LsrB AI-2 LsrR trans-aconitate lsrRK_mRNA AI-2 P-TPO Tam (R)-THMF LsrF


Gensor Unit       
Name: LsrR
Description: Molecular biology level
Detailed: In the absence of 4,5-dihydroxy-2,3-pentanedione, LsrR negatively regulates the expression of genes required for trans-aconitate degradation and (R)-THMF (a 4,5-dihydroxy-2,3-pentanedione precursor) transport and 4,5-dihydroxy-2,3-pentanedione degradation. Under these conditions, LsrR remains unmodified, binds its target sites, preventing its own expression and the expression of its regulated genes. In the presence of 4, 5-dihydroxi-2, 3-pentanedione, 4,5-dihydroxy-2,3-pentanedione-P is generated from (R)-THMF transport and conversion to 4,5-dihydroxy-2,3-pentanedione-P, LsrR is modified to LsrR-4,5-dihydroxy-2,3-pentanedione-P, unbinds its target sites enabling its own expression and the expression of its regulated genes.
Less detailed: In the absence of 4,5-dihydroxy-2,3-pentanedione-P, LsrR negativley regulates the expression of genes needed for trans-aconitate degradation and (R)-THMF (a 4,5-dihydroxy-2,3-pentanedione precursor) transport and 4,5-dihydroxy-2,3-pentanedione degradation.

Physiology level
Detailed: The presence 4,5-dihydroxy-2,3-pentanedione-P induces the expression of genes involved with trans-aconitate degradation and (R)-THMF (a 4,5-dihydroxy-2,3-pentanedione precursor) transport and 4,5-dihydroxy-2,3-pentanedione degradation. Under these conditions, the genes necessary for (R)-THMF transport, conversion of 4,5-dihydroxy-2,3-pentanedione to DHAP and trans-aconitate to monomethyl-ester-of trans-aconitate are expressed.
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Classification:
Groups: Transcription
Carbohydrate transport and metabolism
Energy production and conversion
Gene Ontology Terms (GO): biological_process GO:1905887 - autoinducer AI-2 transmembrane transport
GO:0005975 - carbohydrate metabolic process
GO:0032259 - methylation
GO:0015749 - monosaccharide transmembrane transport
GO:0016310 - phosphorylation
GO:0009372 - quorum sensing
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cellular_component GO:0043190 - ATP-binding cassette (ABC) transporter complex
GO:0005737 - cytoplasm
GO:0005829 - cytosol
GO:0016021 - integral component of membrane
GO:0016020 - membrane
GO:0030288 - outer membrane-bounded periplasmic space
GO:0042597 - periplasmic space
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molecular_function GO:0002952 - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase activity
GO:0005524 - ATP binding
GO:0016887 - ATP hydrolysis activity
GO:0042626 - ATPase-coupled transmembrane transporter activity
GO:0003677 - DNA binding
GO:0016747 - acyltransferase activity, transferring groups other than amino-acyl groups
GO:0016832 - aldehyde-lyase activity
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Components:
  Function Name Type
  simple_molecule (4S)-4-hydroxy-2,3-pentanedione 5-phosphate simple_molecule
  simple_molecule (4S)-4-hydroxy-5-phosphooxypentane-2,3-dione simple_molecule
  simple_molecule (R)-THMF simple_molecule
  simple_molecule (R)-THMF_Ext simple_molecule
  simple_molecule 3-hydroxy-2,4-pentanedione phosphate simple_molecule
  simple_molecule AI-2 simple_molecule
  simple_molecule DHAP simple_molecule
  protein LsrA protein
  protein LsrB protein
  complex LsrB-LsrD-LsrC-LsrA complex
  protein LsrC protein
  protein LsrD protein
  protein LsrF protein
  protein LsrG protein
  protein LsrK protein
  protein LsrR protein
  complex LsrR-(4S)-4-hydroxy-5-phosphooxypentane-2,3-dione complex
  complex LsrR-(R)-THMF complex
  complex LsrR-AI-2 complex
  simple_molecule P-TPO simple_molecule
  simple_molecule SAM simple_molecule
  protein Tam protein
  simple_molecule acetyl-CoA simple_molecule
  simple_molecule adenosylhomocysteine simple_molecule
  simple_molecule coenzyme A simple_molecule
  gene lsrACDBFG-tam gene
  RNA lsrACDBFG-tam_mRNA RNA
  gene lsrRK gene
  RNA lsrRK_mRNA RNA
  simple_molecule monomethyl-ester-of-trans-aconitate simple_molecule
  simple_molecule trans-aconitate simple_molecule


Reactions       
Showing 21 reactions
AI-2 + LsrR LsrR-AI-2
Name: AI-2 + LsrR -> LsrR-AI-2
Description: Reaction transforming AI-2, LsrR into LsrR-AI-2
Type: state transition
Pathway Components: AI-2 + LsrR -> LsrR-AI-2
Components:
Function Name Type
reactant AI-2 simple_molecule
reactant LsrR protein
product LsrR-AI-2 complex
LsrR-AI-2 lsrACDBFG-tam lsrACDBFG-tam_mRNA
Name: lsrACDBFG-tam -> lsrACDBFG-tam_mRNA
Description: Transcription of transcription unit lsrACDBFG-tam. Reaction inhibition by LsrR-AI-2.
Type: transcription
Pathway Components: lsrACDBFG-tam - LsrR-AI-2 -> lsrACDBFG-tam_mRNA
Components:
Function Name Type
inhibitor LsrR-AI-2 complex
reactant lsrACDBFG-tam gene
product lsrACDBFG-tam_mRNA RNA
LsrR-AI-2 lsrRK lsrRK_mRNA
Name: lsrRK -> lsrRK_mRNA
Description: Transcription of transcription unit lsrRK. Reaction inhibition by LsrR-AI-2.
Type: transcription
Pathway Components: lsrRK - LsrR-AI-2 -> lsrRK_mRNA
Components:
Function Name Type
inhibitor LsrR-AI-2 complex
reactant lsrRK gene
product lsrRK_mRNA RNA
(R)-THMF + LsrR LsrR-(R)-THMF
Name: (R)-THMF + LsrR -> LsrR-(R)-THMF
Description: Reaction transforming (R)-THMF, LsrR into LsrR-(R)-THMF
Type: state transition
Pathway Components: (R)-THMF + LsrR -> LsrR-(R)-THMF
Components:
Function Name Type
reactant (R)-THMF simple_molecule
reactant LsrR protein
product LsrR-(R)-THMF complex
TF + effector LsrR-(4S)-4-hydroxy-5-phosphooxypentane-2,3-dione
Name: (4S)-4-hydroxy-5-phosphooxypentane-2,3-dione + LsrR -> LsrR-(4S)-4-hydroxy-5-phosphooxypentane-2,3-dione
Description: Reaction transforming (4S)-4-hydroxy-5-phosphooxypentane-2,3-dione, LsrR into LsrR-(4S)-4-hydroxy-5-phosphooxypentane-2,3-dione
Type: state transition
Pathway Components: (4S)-4-hydroxy-5-phosphooxypentane-2,3-dione + LsrR -> LsrR-(4S)-4-hydroxy-5-phosphooxypentane-2,3-dione
Components:
Function Name Type
reactant (4S)-4-hydroxy-5-phosphooxypentane-2,3-dione simple_molecule
reactant LsrR protein
product LsrR-(4S)-4-hydroxy-5-phosphooxypentane-2,3-dione complex
lsrACDBFG-tam_mRNA LsrA
Name: lsrACDBFG-tam_mRNA -> LsrA
Description: Translation of LsrA
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> LsrA
Components:
Function Name Type
product LsrA protein
reactant lsrACDBFG-tam_mRNA RNA
LsrD + LsrC + LsrB + LsrA LsrB-LsrD-LsrC-LsrA
Name: LsrD + LsrC + LsrB + LsrA -> LsrB-LsrD-LsrC-LsrA
Description: Reaction transforming LsrD, LsrC, LsrB, LsrA into LsrB-LsrD-LsrC-LsrA
Type: state transition
Pathway Components: LsrD + LsrC + LsrB + LsrA -> LsrB-LsrD-LsrC-LsrA
Components:
Function Name Type
reactant LsrA protein
reactant LsrB protein
product LsrB-LsrD-LsrC-LsrA complex
reactant LsrC protein
reactant LsrD protein
LsrB-LsrD-LsrC-LsrA (R)-THMF_Ext (R)-THMF
Name: (R)-THMF_Ext -> (R)-THMF
Description: Transport of (R)-THMF_Ext through a bacterial membrane, leading to the presence of (R)-THMF inside the cell.
Type: transport
Pathway Components: (R)-THMF_Ext - LsrB-LsrD-LsrC-LsrA -> (R)-THMF
Components:
Function Name Type
product (R)-THMF simple_molecule
reactant (R)-THMF_Ext simple_molecule
catalyzer LsrB-LsrD-LsrC-LsrA complex
lsrACDBFG-tam_mRNA LsrC
Name: lsrACDBFG-tam_mRNA -> LsrC
Description: Translation of LsrC
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> LsrC
Components:
Function Name Type
product LsrC protein
reactant lsrACDBFG-tam_mRNA RNA
lsrACDBFG-tam_mRNA LsrD
Name: lsrACDBFG-tam_mRNA -> LsrD
Description: Translation of LsrD
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> LsrD
Components:
Function Name Type
product LsrD protein
reactant lsrACDBFG-tam_mRNA RNA
lsrACDBFG-tam_mRNA LsrB
Name: lsrACDBFG-tam_mRNA -> LsrB
Description: Translation of LsrB
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> LsrB
Components:
Function Name Type
product LsrB protein
reactant lsrACDBFG-tam_mRNA RNA
lsrACDBFG-tam_mRNA LsrF
Name: lsrACDBFG-tam_mRNA -> LsrF
Description: Translation of LsrF
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> LsrF
Components:
Function Name Type
product LsrF protein
reactant lsrACDBFG-tam_mRNA RNA
LsrF 3-hydroxy-2,4-pentanedione phosphate + coenzyme A acetyl-CoA + DHAP
Name: 3-hydroxy-2,4-pentanedione phosphate + coenzyme A -> acetyl-CoA + DHAP
Description: Reaction transforming 3-hydroxy-2,4-pentanedione phosphate, coenzyme A into acetyl-CoA, DHAP
Type: state transition
Pathway Components: 3-hydroxy-2,4-pentanedione phosphate + coenzyme A - LsrF -> acetyl-CoA + DHAP
Components:
Function Name Type
reactant 3-hydroxy-2,4-pentanedione phosphate simple_molecule
product DHAP simple_molecule
catalyzer LsrF protein
product acetyl-CoA simple_molecule
reactant coenzyme A simple_molecule
lsrACDBFG-tam_mRNA LsrG
Name: lsrACDBFG-tam_mRNA -> LsrG
Description: Translation of LsrG
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> LsrG
Components:
Function Name Type
product LsrG protein
reactant lsrACDBFG-tam_mRNA RNA
LsrG (4S)-4-hydroxy-2,3-pentanedione 5-phosphate 3-hydroxy-2,4-pentanedione phosphate
Name: (4S)-4-hydroxy-2,3-pentanedione 5-phosphate -> 3-hydroxy-2,4-pentanedione phosphate
Description: Reaction transforming (4S)-4-hydroxy-2,3-pentanedione 5-phosphate into 3-hydroxy-2,4-pentanedione phosphate
Type: state transition
Pathway Components: (4S)-4-hydroxy-2,3-pentanedione 5-phosphate - LsrG -> 3-hydroxy-2,4-pentanedione phosphate
Components:
Function Name Type
reactant (4S)-4-hydroxy-2,3-pentanedione 5-phosphate simple_molecule
product 3-hydroxy-2,4-pentanedione phosphate simple_molecule
catalyzer LsrG protein
LsrG 3-hydroxy-2,4-pentanedione phosphate P-TPO
Name: 3-hydroxy-2,4-pentanedione phosphate -> P-TPO
Description: Reaction transforming 3-hydroxy-2,4-pentanedione phosphate into P-TPO
Type: state transition
Pathway Components: 3-hydroxy-2,4-pentanedione phosphate - LsrG -> P-TPO
Components:
Function Name Type
reactant 3-hydroxy-2,4-pentanedione phosphate simple_molecule
catalyzer LsrG protein
product P-TPO simple_molecule
lsrACDBFG-tam_mRNA Tam
Name: lsrACDBFG-tam_mRNA -> Tam
Description: Translation of Tam
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> Tam
Components:
Function Name Type
product Tam protein
reactant lsrACDBFG-tam_mRNA RNA
Tam adenosylhomocysteine trans-aconitate + SAM Products
Name: trans-aconitate + SAM -> monomethyl-ester-of-trans-aconitate + adenosylhomocysteine
Description: Reaction transforming trans-aconitate, SAM into monomethyl-ester-of-trans-aconitate, adenosylhomocysteine
Type: state transition
Pathway Components: trans-aconitate + SAM - Tam - adenosylhomocysteine -> monomethyl-ester-of-trans-aconitate + adenosylhomocysteine
Components:
Function Name Type
reactant SAM simple_molecule
catalyzer Tam protein
inhibitor adenosylhomocysteine simple_molecule
product adenosylhomocysteine simple_molecule
product monomethyl-ester-of-trans-aconitate simple_molecule
reactant trans-aconitate simple_molecule
lsrRK_mRNA LsrR
Name: lsrRK_mRNA -> LsrR
Description: Translation of LsrR
Type: translation
Pathway Components: lsrRK_mRNA -> LsrR
Components:
Function Name Type
product LsrR protein
reactant lsrRK_mRNA RNA
lsrRK_mRNA LsrK
Name: lsrRK_mRNA -> LsrK
Description: Translation of LsrK
Type: translation
Pathway Components: lsrRK_mRNA -> LsrK
Components:
Function Name Type
product LsrK protein
reactant lsrRK_mRNA RNA
LsrK AI-2 (4S)-4-hydroxy-2,3-pentanedione 5-phosphate
Name: AI-2 -> (4S)-4-hydroxy-2,3-pentanedione 5-phosphate
Description: Reaction transforming AI-2 into (4S)-4-hydroxy-2,3-pentanedione 5-phosphate
Type: state transition
Pathway Components: AI-2 - LsrK -> (4S)-4-hydroxy-2,3-pentanedione 5-phosphate
Components:
Function Name Type
product (4S)-4-hydroxy-2,3-pentanedione 5-phosphate simple_molecule
reactant AI-2 simple_molecule
catalyzer LsrK protein
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