RegulonDB RegulonDB

LsrR Genetic Sensory Response Unit in Escherichia coli K-12 genome


Gensor Unit Map          
(R)-THMF (R)-THMF_Ext monomethyl-ester-of-trans-aconitate 3,4,4-trihydroxy-2-pentanone-5-phosphate 4,5-dihydroxy-2,3-pentanedione 4,5-dihydroxy-2,3-pentanedione-P 4-hydroxy-2,3-pentanedione-5-phosphate LsrA LsrB LsrC LsrD LsrF LsrG LsrK LsrR S-adenosyl-L-homocysteine S-adenosyl-L-methionine Tam lsrACDBFG-tam lsrACDBFG-tam_mRNA lsrRK lsrRK_mRNA phosphate trans-aconitate LsrR 4,5-dihydroxy-2,3-pentanedione-P LsrR 4,5-dihydroxy-2,3-pentanedione LsrB LsrD LsrC LsrA 3-hydroxy-2,4-pentanedione phosphate DHAP


Gensor Unit       
Name: LsrR
Description: Molecular biology level
Detailed: In the absence of 4,5-dihydroxy-2,3-pentanedione, LsrR negatively regulates the expression of genes required for trans-aconitate degradation and (R)-THMF (a 4,5-dihydroxy-2,3-pentanedione precursor) transport and 4,5-dihydroxy-2,3-pentanedione degradation. Under these conditions, LsrR remains unmodified, binds its target sites, preventing its own expression and the expression of its regulated genes. In the presence of 4, 5-dihydroxi-2, 3-pentanedione, 4,5-dihydroxy-2,3-pentanedione-P is generated from (R)-THMF transport and conversion to 4,5-dihydroxy-2,3-pentanedione-P, LsrR is modified to LsrR-4,5-dihydroxy-2,3-pentanedione-P, unbinds its target sites enabling its own expression and the expression of its regulated genes.
Less detailed: In the absence of 4,5-dihydroxy-2,3-pentanedione-P, LsrR negativley regulates the expression of genes needed for trans-aconitate degradation and (R)-THMF (a 4,5-dihydroxy-2,3-pentanedione precursor) transport and 4,5-dihydroxy-2,3-pentanedione degradation.

Physiology level
Detailed: The presence 4,5-dihydroxy-2,3-pentanedione-P induces the expression of genes involved with trans-aconitate degradation and (R)-THMF (a 4,5-dihydroxy-2,3-pentanedione precursor) transport and 4,5-dihydroxy-2,3-pentanedione degradation. Under these conditions, the genes necessary for (R)-THMF transport, conversion of 4,5-dihydroxy-2,3-pentanedione to DHAP and trans-aconitate to monomethyl-ester-of trans-aconitate are expressed.
Less detailed: The presence of 4,5-dihydroxy-2,3-pentanedione-P induces the expression of genes needful for (R)-THMF transport, conversion of 4,5-dihydroxy-2,3-pentanedione to DHAP and trans-aconitate to monomethyl-ester-of trans-aconitate. Read more >
Classification:
Groups: Unclassified Gensor Units
Gene Ontology Terms (GO): biological_process GO:0015749 - monosaccharide transmembrane transport
GO:0055114 - oxidation-reduction process
GO:0009372 - quorum sensing
GO:2000142 - regulation of DNA-templated transcription, initiation
GO:0009408 - response to heat
GO:0044010 - single-species biofilm formation
Read more >
cellular_component GO:0043190 - ATP-binding cassette (ABC) transporter complex
GO:0005829 - cytosol
GO:0016021 - integral component of membrane
GO:0042597 - periplasmic space
GO:0005886 - plasma membrane
molecular_function GO:0002952 - (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase activity
GO:0016832 - aldehyde-lyase activity
GO:0071518 - autoinducer-2 kinase activity
GO:0000986 - bacterial-type proximal promoter sequence-specific DNA binding
GO:0003824 - catalytic activity
GO:0042802 - identical protein binding
GO:0016853 - isomerase activity
Read more >
Components: Show/Hide Components Table (30 more...)
Function Name Type
simple_molecule (4S)-4-hydroxy-2,3-pentanedione 5-phosphate simple_molecule
simple_molecule (R)-THMF simple_molecule
simple_molecule (R)-THMF_Ext simple_molecule
simple_molecule 3-hydroxy-2,4-pentanedione phosphate simple_molecule
simple_molecule AI-2 simple_molecule
simple_molecule DHAP simple_molecule
protein LsrA protein
protein LsrB protein
complex LsrB-LsrD-LsrC-LsrA complex
protein LsrC protein
protein LsrD protein
protein LsrF protein
protein LsrG protein
protein LsrK protein
protein LsrR protein
complex LsrR-AI-2 complex
complex LsrR-autoinducer 2-P complex
simple_molecule P-TPO simple_molecule
simple_molecule SAM simple_molecule
protein Tam protein
simple_molecule acetyl-CoA simple_molecule
simple_molecule adenosylhomocysteine simple_molecule
simple_molecule autoinducer 2-P simple_molecule
simple_molecule coenzyme A simple_molecule
gene lsrACDBFG-tam gene
RNA lsrACDBFG-tam_mRNA RNA
gene lsrRK gene
RNA lsrRK_mRNA RNA
simple_molecule monomethyl-ester-of-trans-aconitate simple_molecule
simple_molecule trans-aconitate simple_molecule


Reactions       
Showing 20 reactions
AI-2 + LsrR LsrR-AI-2
Name: AI-2 + LsrR -> LsrR-AI-2
Description: Reaction transforming AI-2, LsrR into LsrR-AI-2
Type: state transition
Pathway Components: AI-2 + LsrR -> LsrR-AI-2
Components:
Function Name Type Evidence Reference(s)
reactant AI-2 simple_molecule nd nd
reactant LsrR protein nd nd
product LsrR-AI-2 complex nd nd
LsrR-AI-2 lsrACDBFG-tam lsrACDBFG-tam_mRNA
Name: lsrACDBFG-tam -> lsrACDBFG-tam_mRNA
Description: Transcription of transcription unit lsrACDBFG-tam. Reaction inhibition by LsrR-AI-2.
Type: transcription
Pathway Components: lsrACDBFG-tam - LsrR-AI-2 -> lsrACDBFG-tam_mRNA
Components:
Function Name Type Evidence Reference(s)
inhibitor LsrR-AI-2 complex nd nd
reactant lsrACDBFG-tam gene nd nd
product lsrACDBFG-tam_mRNA RNA nd nd
LsrR-AI-2 lsrRK lsrRK_mRNA
Name: lsrRK -> lsrRK_mRNA
Description: Transcription of transcription unit lsrRK. Reaction inhibition by LsrR-AI-2.
Type: transcription
Pathway Components: lsrRK - LsrR-AI-2 -> lsrRK_mRNA
Components:
Function Name Type Evidence Reference(s)
inhibitor LsrR-AI-2 complex nd nd
reactant lsrRK gene nd nd
product lsrRK_mRNA RNA nd nd
autoinducer 2-P + LsrR LsrR-autoinducer 2-P
Name: autoinducer 2-P + LsrR -> LsrR-autoinducer 2-P
Description: Reaction transforming autoinducer 2-P, LsrR into LsrR-autoinducer 2-P
Type: state transition
Pathway Components: autoinducer 2-P + LsrR -> LsrR-autoinducer 2-P
Components:
Function Name Type Evidence Reference(s)
reactant LsrR protein nd nd
product LsrR-autoinducer 2-P complex nd nd
reactant autoinducer 2-P simple_molecule nd nd
lsrACDBFG-tam_mRNA LsrA
Name: lsrACDBFG-tam_mRNA -> LsrA
Description: Translation of LsrA
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> LsrA
Components:
Function Name Type Evidence Reference(s)
product LsrA protein nd nd
reactant lsrACDBFG-tam_mRNA RNA nd nd
LsrB + LsrD + LsrA + LsrC LsrB-LsrD-LsrC-LsrA
Name: LsrB + LsrD + LsrA + LsrC -> LsrB-LsrD-LsrC-LsrA
Description: Reaction transforming LsrB, LsrD, LsrA, LsrC into LsrB-LsrD-LsrC-LsrA
Type: state transition
Pathway Components: LsrB + LsrD + LsrA + LsrC -> LsrB-LsrD-LsrC-LsrA
Components:
Function Name Type Evidence Reference(s)
reactant LsrA protein nd nd
reactant LsrB protein nd nd
product LsrB-LsrD-LsrC-LsrA complex nd nd
reactant LsrC protein nd nd
reactant LsrD protein nd nd
LsrB-LsrD-LsrC-LsrA (R)-THMF_Ext (R)-THMF
Name: (R)-THMF_Ext -> (R)-THMF
Description: Transport of (R)-THMF_Ext through a bacterial membrane, leading to the presence of (R)-THMF inside the cell.
Type: transport
Pathway Components: (R)-THMF_Ext - LsrB-LsrD-LsrC-LsrA -> (R)-THMF
Components:
Function Name Type Evidence Reference(s)
product (R)-THMF simple_molecule nd nd
reactant (R)-THMF_Ext simple_molecule nd nd
catalyzer LsrB-LsrD-LsrC-LsrA complex nd nd
lsrACDBFG-tam_mRNA LsrC
Name: lsrACDBFG-tam_mRNA -> LsrC
Description: Translation of LsrC
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> LsrC
Components:
Function Name Type Evidence Reference(s)
product LsrC protein nd nd
reactant lsrACDBFG-tam_mRNA RNA nd nd
lsrACDBFG-tam_mRNA LsrD
Name: lsrACDBFG-tam_mRNA -> LsrD
Description: Translation of LsrD
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> LsrD
Components:
Function Name Type Evidence Reference(s)
product LsrD protein nd nd
reactant lsrACDBFG-tam_mRNA RNA nd nd
lsrACDBFG-tam_mRNA LsrB
Name: lsrACDBFG-tam_mRNA -> LsrB
Description: Translation of LsrB
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> LsrB
Components:
Function Name Type Evidence Reference(s)
product LsrB protein nd nd
reactant lsrACDBFG-tam_mRNA RNA nd nd
lsrACDBFG-tam_mRNA LsrF
Name: lsrACDBFG-tam_mRNA -> LsrF
Description: Translation of LsrF
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> LsrF
Components:
Function Name Type Evidence Reference(s)
product LsrF protein nd nd
reactant lsrACDBFG-tam_mRNA RNA nd nd
LsrF 3-hydroxy-2,4-pentanedione phosphate + coenzyme A acetyl-CoA + DHAP
Name: 3-hydroxy-2,4-pentanedione phosphate + coenzyme A -> acetyl-CoA + DHAP
Description: Reaction transforming 3-hydroxy-2,4-pentanedione phosphate, coenzyme A into acetyl-CoA, DHAP
Type: state transition
Pathway Components: 3-hydroxy-2,4-pentanedione phosphate + coenzyme A - LsrF -> acetyl-CoA + DHAP
Components:
Function Name Type Evidence Reference(s)
reactant 3-hydroxy-2,4-pentanedione phosphate simple_molecule nd nd
product DHAP simple_molecule nd nd
catalyzer LsrF protein nd nd
product acetyl-CoA simple_molecule nd nd
reactant coenzyme A simple_molecule nd nd
lsrACDBFG-tam_mRNA LsrG
Name: lsrACDBFG-tam_mRNA -> LsrG
Description: Translation of LsrG
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> LsrG
Components:
Function Name Type Evidence Reference(s)
product LsrG protein nd nd
reactant lsrACDBFG-tam_mRNA RNA nd nd
LsrG (4S)-4-hydroxy-2,3-pentanedione 5-phosphate 3-hydroxy-2,4-pentanedione phosphate
Name: (4S)-4-hydroxy-2,3-pentanedione 5-phosphate -> 3-hydroxy-2,4-pentanedione phosphate
Description: Reaction transforming (4S)-4-hydroxy-2,3-pentanedione 5-phosphate into 3-hydroxy-2,4-pentanedione phosphate
Type: state transition
Pathway Components: (4S)-4-hydroxy-2,3-pentanedione 5-phosphate - LsrG -> 3-hydroxy-2,4-pentanedione phosphate
Components:
Function Name Type Evidence Reference(s)
reactant (4S)-4-hydroxy-2,3-pentanedione 5-phosphate simple_molecule nd nd
product 3-hydroxy-2,4-pentanedione phosphate simple_molecule nd nd
catalyzer LsrG protein nd nd
LsrG 3-hydroxy-2,4-pentanedione phosphate P-TPO
Name: 3-hydroxy-2,4-pentanedione phosphate -> P-TPO
Description: Reaction transforming 3-hydroxy-2,4-pentanedione phosphate into P-TPO
Type: state transition
Pathway Components: 3-hydroxy-2,4-pentanedione phosphate - LsrG -> P-TPO
Components:
Function Name Type Evidence Reference(s)
reactant 3-hydroxy-2,4-pentanedione phosphate simple_molecule nd nd
catalyzer LsrG protein nd nd
product P-TPO simple_molecule nd nd
lsrACDBFG-tam_mRNA Tam
Name: lsrACDBFG-tam_mRNA -> Tam
Description: Translation of Tam
Type: translation
Pathway Components: lsrACDBFG-tam_mRNA -> Tam
Components:
Function Name Type Evidence Reference(s)
product Tam protein nd nd
reactant lsrACDBFG-tam_mRNA RNA nd nd
Tam adenosylhomocysteine trans-aconitate + SAM Products
Name: trans-aconitate + SAM -> monomethyl-ester-of-trans-aconitate + adenosylhomocysteine
Description: Reaction transforming trans-aconitate, SAM into monomethyl-ester-of-trans-aconitate, adenosylhomocysteine
Type: state transition
Pathway Components: trans-aconitate + SAM - Tam - adenosylhomocysteine -> monomethyl-ester-of-trans-aconitate + adenosylhomocysteine
Components:
Function Name Type Evidence Reference(s)
reactant SAM simple_molecule nd nd
catalyzer Tam protein nd nd
inhibitor adenosylhomocysteine simple_molecule nd nd
product adenosylhomocysteine simple_molecule nd nd
product monomethyl-ester-of-trans-aconitate simple_molecule nd nd
reactant trans-aconitate simple_molecule nd nd
lsrRK_mRNA LsrR
Name: lsrRK_mRNA -> LsrR
Description: Translation of LsrR
Type: translation
Pathway Components: lsrRK_mRNA -> LsrR
Components:
Function Name Type Evidence Reference(s)
product LsrR protein nd nd
reactant lsrRK_mRNA RNA nd nd
lsrRK_mRNA LsrK
Name: lsrRK_mRNA -> LsrK
Description: Translation of LsrK
Type: translation
Pathway Components: lsrRK_mRNA -> LsrK
Components:
Function Name Type Evidence Reference(s)
product LsrK protein nd nd
reactant lsrRK_mRNA RNA nd nd
LsrK AI-2 (4S)-4-hydroxy-2,3-pentanedione 5-phosphate
Name: AI-2 -> (4S)-4-hydroxy-2,3-pentanedione 5-phosphate
Description: Reaction transforming AI-2 into (4S)-4-hydroxy-2,3-pentanedione 5-phosphate
Type: state transition
Pathway Components: AI-2 - LsrK -> (4S)-4-hydroxy-2,3-pentanedione 5-phosphate
Components:
Function Name Type Evidence Reference(s)
product (4S)-4-hydroxy-2,3-pentanedione 5-phosphate simple_molecule nd nd
reactant AI-2 simple_molecule nd nd
catalyzer LsrK protein nd nd



RegulonDB