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BirA Genetic Sensory Response Unit in Escherichia coli K-12 genome


Gensor Unit Map          
7,8-diaminopelargonate 8-amino-7-oxononanoate BioA BioB BioC BioD BioF BirA L-alanine S-adenosyl-4-methylthio-2-oxobutanoate S-adenosyl-L-homocysteine S-adenosyl-L-methionine holo-[ACP] malonyl-[ACP] malonyl-[ACP] methyl ester pimeloyl-[ACP] biotinyl-5'-adenylate bioA bioA_mRNA bioBFCD bioBFCD_mRNA biotin CoA dethiobiotin pimeloyl-CoA BirA biotinyl-5'-adenylate AccB AccB biotin S-adenosyl-L-methionine S-adenosyl-L-methionine L-methionine 5'-deoxyadenosine


Gensor Unit       
Name: BirA
Description: Molecular biology level
Detailed: In the presence of biotin-5'-AMP, BirA negatively regulates the expression of genes required for biotin biosynthetic process. Under these conditions, BirA is modified to BirA-Biotynil-5-adenylate, binds its target sites preventing the expression of its regulated genes. In the absence of biotin-5'-AMP, BirA unbinds its target sites enabling the expression of its regulated genes.
Less detailed: In the presence of biotin-5'-AMP, BirA negatively regulates the expression of genes needed for in biotin biosynthetic process.

Physiology level
Detailed: The presence of biotin inhibits the expression of genes involved with biotin biosynthesis. Under these conditions the genes necessary for the conversion from malonyl-[ACP] through biotin are not expressed.
Less detailed: The presence of biotin-5'-AMP inhibits the expression of genes needful for the conversion from malonyl-ACP to biotin.

With enzymatic activity
Molecular biology level
Detailed: BirA is a dual-function protein, it is a transcriptional factor but also harbors enzymatic activity of biotin ligase. In the presence of biotin-5'-AMP BirA negatively regulates the expression of genes required for biotin biosynthetic process. Under these conditions, BirA is modified to BirA-Biotynil-5-adenylate, binds its target sites preventing (or avoiding or hindering) the expression of its regulated genes.
Less detailed: BirA dual-function protein is a transcriptional factor that also harbors enzymatic activity of biotin ligase.
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Classification:
Groups: Unclassified Gensor Units
Gene Ontology Terms (GO): biological_process GO:0009102 - biotin biosynthetic process
cellular_component GO:0005829 - cytosol
molecular_function GO:0051537 - 2 iron, 2 sulfur cluster binding
GO:0051539 - 4 iron, 4 sulfur cluster binding
GO:0008710 - 8-amino-7-oxononanoate synthase activity
GO:0005524 - ATP binding
GO:0004015 - adenosylmethionine-8-amino-7-oxononanoate transaminase activity
GO:0004076 - biotin synthase activity
GO:0010340 - carboxyl-O-methyltransferase activity
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Components: Show/Hide Components Table (34 more...)
Function Name Type
simple_molecule 5'-deoxyadenosine simple_molecule
simple_molecule AcpP simple_molecule
protein BioA protein
protein BioB protein
protein BioC protein
protein BioD protein
protein BioF protein
protein BirA protein
complex BirA-biotinyl-5'-AMP complex
simple_molecule CO2 simple_molecule
simple_molecule DAPA simple_molecule
simple_molecule KAPA simple_molecule
simple_molecule S-adenosyl-4-methylthio-2-oxobutanoate simple_molecule
simple_molecule SAM simple_molecule
simple_molecule adenosylhomocysteine simple_molecule
simple_molecule alanine simple_molecule
gene bioA gene
RNA bioA_mRNA RNA
gene bioBFCD gene
RNA bioBFCD_mRNA RNA
simple_molecule biotin simple_molecule
simple_molecule biotinyl-5'-AMP simple_molecule
simple_molecule coenzyme A simple_molecule
simple_molecule dethiobiotin simple_molecule
simple_molecule malonyl-S-ACP simple_molecule
simple_molecule malonyl-[acp] methyl ester simple_molecule
simple_molecule methionine simple_molecule
simple_molecule oxidized [2Fe-2S] ferredoxin simple_molecule
simple_molecule phosphate simple_molecule
simple_molecule pimelyl-CoA simple_molecule
simple_molecule pimelyl-[acp] simple_molecule
simple_molecule reduced [2Fe-2S] ferredoxin simple_molecule
simple_molecule sulfurated sulfur donor simple_molecule
simple_molecule unsulfurated [sulfur donor] simple_molecule


Reactions       
Showing 15 reactions
biotinyl-5'-AMP + BirA BirA-biotinyl-5'-AMP
Name: biotinyl-5'-AMP + BirA -> BirA-biotinyl-5'-AMP
Description: Reaction transforming biotinyl-5'-AMP, BirA into BirA-biotinyl-5'-AMP
Type: state transition
Pathway Components: biotinyl-5'-AMP + BirA -> BirA-biotinyl-5'-AMP
Components:
Function Name Type Evidence Reference(s)
reactant BirA protein nd nd
product BirA-biotinyl-5'-AMP complex nd nd
reactant biotinyl-5'-AMP simple_molecule nd nd
BirA-biotinyl-5'-AMP bioA bioA_mRNA
Name: bioA -> bioA_mRNA
Description: Transcription of transcription unit bioA. Reaction inhibition by BirA-biotinyl-5'-AMP.
Type: transcription
Pathway Components: bioA - BirA-biotinyl-5'-AMP -> bioA_mRNA
Components:
Function Name Type Evidence Reference(s)
inhibitor BirA-biotinyl-5'-AMP complex nd nd
reactant bioA gene nd nd
product bioA_mRNA RNA nd nd
BirA-biotinyl-5'-AMP bioBFCD bioBFCD_mRNA
Name: bioBFCD -> bioBFCD_mRNA
Description: Transcription of transcription unit bioBFCD. Reaction inhibition by BirA-biotinyl-5'-AMP.
Type: transcription
Pathway Components: bioBFCD - BirA-biotinyl-5'-AMP -> bioBFCD_mRNA
Components:
Function Name Type Evidence Reference(s)
inhibitor BirA-biotinyl-5'-AMP complex nd nd
reactant bioBFCD gene nd nd
product bioBFCD_mRNA RNA nd nd
bioBFCD_mRNA BioB
Name: bioBFCD_mRNA -> BioB
Description: Translation of BioB
Type: translation
Pathway Components: bioBFCD_mRNA -> BioB
Components:
Function Name Type Evidence Reference(s)
product BioB protein nd nd
reactant bioBFCD_mRNA RNA nd nd
5'-deoxyadenosine BioB adenosylhomocysteine methionine Reactants Products
Name: reduced [2Fe-2S] ferredoxin + sulfurated sulfur donor + dethiobiotin + SAM -> oxidized [2Fe-2S] ferredoxin + unsulfurated [sulfur donor] + 5'-deoxyadenosine + methionine + biotin
Description: Reaction transforming reduced [2Fe-2S] ferredoxin, sulfurated sulfur donor, dethiobiotin, SAM into oxidized [2Fe-2S] ferredoxin, unsulfurated [sulfur donor] , 5'-deoxyadenosine , methionine , biotin
Type: state transition
Pathway Components: reduced [2Fe-2S] ferredoxin + sulfurated sulfur donor + dethiobiotin + SAM - BioB - 5'-deoxyadenosine - adenosylhomocysteine - methionine -> oxidized [2Fe-2S] ferredoxin + unsulfurated [sulfur donor] + 5'-deoxyadenosine + methionine + biotin
Components: Show/Hide Components Table
bioBFCD_mRNA BioF
Name: bioBFCD_mRNA -> BioF
Description: Translation of BioF
Type: translation
Pathway Components: bioBFCD_mRNA -> BioF
Components:
Function Name Type Evidence Reference(s)
product BioF protein nd nd
reactant bioBFCD_mRNA RNA nd nd
BioF pimelyl-[acp] + alanine AcpP + KAPA + CO2
Name: pimelyl-[acp] + alanine -> AcpP + KAPA + CO2
Description: Reaction transforming pimelyl-[acp], alanine into AcpP, KAPA , CO2
Type: state transition
Pathway Components: pimelyl-[acp] + alanine - BioF -> AcpP + KAPA + CO2
Components:
Function Name Type Evidence Reference(s)
product AcpP simple_molecule nd nd
catalyzer BioF protein nd nd
product CO2 simple_molecule nd nd
product KAPA simple_molecule nd nd
reactant alanine simple_molecule nd nd
reactant pimelyl-[acp] simple_molecule nd nd
BioF pimelyl-CoA + alanine coenzyme A + KAPA + CO2
Name: pimelyl-CoA + alanine -> coenzyme A + KAPA + CO2
Description: Reaction transforming pimelyl-CoA, alanine into coenzyme A, KAPA , CO2
Type: state transition
Pathway Components: pimelyl-CoA + alanine - BioF -> coenzyme A + KAPA + CO2
Components:
Function Name Type Evidence Reference(s)
catalyzer BioF protein nd nd
product CO2 simple_molecule nd nd
product KAPA simple_molecule nd nd
reactant alanine simple_molecule nd nd
product coenzyme A simple_molecule nd nd
reactant pimelyl-CoA simple_molecule nd nd
bioBFCD_mRNA BioC
Name: bioBFCD_mRNA -> BioC
Description: Translation of BioC
Type: translation
Pathway Components: bioBFCD_mRNA -> BioC
Components:
Function Name Type Evidence Reference(s)
product BioC protein nd nd
reactant bioBFCD_mRNA RNA nd nd
BioC malonyl-S-ACP + SAM malonyl-[acp] methyl ester + adenosylhomocysteine
Name: malonyl-S-ACP + SAM -> malonyl-[acp] methyl ester + adenosylhomocysteine
Description: Reaction transforming malonyl-S-ACP, SAM into malonyl-[acp] methyl ester, adenosylhomocysteine
Type: state transition
Pathway Components: malonyl-S-ACP + SAM - BioC -> malonyl-[acp] methyl ester + adenosylhomocysteine
Components:
Function Name Type Evidence Reference(s)
catalyzer BioC protein nd nd
reactant SAM simple_molecule nd nd
product adenosylhomocysteine simple_molecule nd nd
reactant malonyl-S-ACP simple_molecule nd nd
product malonyl-[acp] methyl ester simple_molecule nd nd
bioBFCD_mRNA BioD
Name: bioBFCD_mRNA -> BioD
Description: Translation of BioD
Type: translation
Pathway Components: bioBFCD_mRNA -> BioD
Components:
Function Name Type Evidence Reference(s)
product BioD protein nd nd
reactant bioBFCD_mRNA RNA nd nd
BioD DAPA + CO2 dethiobiotin + phosphate
Name: DAPA + CO2 -> dethiobiotin + phosphate
Description: Reaction transforming DAPA, CO2 into dethiobiotin, phosphate
Type: state transition
Pathway Components: DAPA + CO2 - BioD -> dethiobiotin + phosphate
Components:
Function Name Type Evidence Reference(s)
catalyzer BioD protein nd nd
reactant CO2 simple_molecule nd nd
reactant DAPA simple_molecule nd nd
product dethiobiotin simple_molecule nd nd
product phosphate simple_molecule nd nd
bioA_mRNA BioA
Name: bioA_mRNA -> BioA
Description: Translation of BioA
Type: translation
Pathway Components: bioA_mRNA -> BioA
Components:
Function Name Type Evidence Reference(s)
product BioA protein nd nd
reactant bioA_mRNA RNA nd nd
BioA KAPA KAPA + SAM S-adenosyl-4-methylthio-2-oxobutanoate + DAPA
Name: KAPA + SAM -> S-adenosyl-4-methylthio-2-oxobutanoate + DAPA
Description: Reaction transforming KAPA, SAM into S-adenosyl-4-methylthio-2-oxobutanoate, DAPA
Type: state transition
Pathway Components: KAPA + SAM - BioA - KAPA -> S-adenosyl-4-methylthio-2-oxobutanoate + DAPA
Components:
Function Name Type Evidence Reference(s)
catalyzer BioA protein nd nd
product DAPA simple_molecule nd nd
inhibitor KAPA simple_molecule nd nd
reactant KAPA simple_molecule nd nd
product S-adenosyl-4-methylthio-2-oxobutanoate simple_molecule nd nd
reactant SAM simple_molecule nd nd
malonyl-[acp] methyl ester pimelyl-[acp]
Name: malonyl-[acp] methyl ester -> pimelyl-[acp]
Description: Transformation of malonyl-[acp] methyl ester into pimelyl-[acp] by one or more reactions not shown.
Type: complementary pathway reaction
Pathway Components: malonyl-[acp] methyl ester / RXN-11474 / RXN-11476 / RXN-11477 / RXN-11478 / RXN-11479 / RXN-11480 / RXN-11481 / RXN-11482 / RXN-11483 / pimelyl-[acp]
Components:
Function Name Type Evidence Reference(s)
reactant malonyl-[acp] methyl ester simple_molecule nd nd
product pimelyl-[acp] simple_molecule nd nd



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