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AlaS Genetic Sensory Response Unit in Escherichia coli K-12 genome


Gensor Unit Map          
ALANYL-TRNA(ALA) alaS alaS_mRNA AlaS alanine TRNA(ALA) alanine AlaS diphosphate


Gensor Unit       
Name: AlaS
Description: Molecular biology level
Detailed: In the presence of alanine AlaS, a dual protein with tRNA aminoacylation enzymatic and DNA binding activities, negatively regulates the expression of genes required to alanyl tRNA acylation process. Under these conditions, AlaS is modified to AlaS-alanine, binds its target sites preventing its own expression.
Less detailed: In the presence of alanine, AlaS negatively regulates the expression of genes needed for tRNA aminocylation.

Physiology level
Detailed: The presence of alanine inhibits the expression of genes related with tRNA aminoacylation process. Under this condition the gene necessary for tRNA-Ala biosynthesis is not expressed.
Less detailed: The presence of alanine inhibits the expression of the gene that mediates tRNA alanine acylation.
Classification:
Groups: Translation, ribosomal structure and biogenesis
Gene Ontology Terms (GO): biological_process GO:0006419 - alanyl-tRNA aminoacylation
GO:0106074 - aminoacyl-tRNA metabolism involved in translational fidelity
GO:0045892 - negative regulation of transcription, DNA-templated
GO:0043039 - tRNA aminoacylation
GO:0006400 - tRNA modification
GO:0006412 - translation
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cellular_component GO:0005737 - cytoplasm
GO:0005829 - cytosol
molecular_function GO:0005524 - ATP binding
GO:0003723 - RNA binding
GO:0002196 - Ser-tRNA(Ala) hydrolase activity
GO:0004813 - alanine-tRNA ligase activity
GO:0016597 - amino acid binding
GO:0002161 - aminoacyl-tRNA editing activity
GO:0004812 - aminoacyl-tRNA ligase activity
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Components:
  Function Name Type
  simple_molecule ALANYL-TRNA(ALA) simple_molecule
  protein AlaS protein
  complex AlaS-alanine complex
  simple_molecule TRNA(ALA) simple_molecule
  gene alaS gene
  RNA alaS_mRNA RNA
  simple_molecule alanine simple_molecule
  simple_molecule diphosphate simple_molecule


Reactions       
Showing 4 reactions
alanine + AlaS AlaS-alanine
Name: alanine + AlaS -> AlaS-alanine
Description: Reaction transforming alanine, AlaS into AlaS-alanine
Type: state transition
Pathway Components: alanine + AlaS -> AlaS-alanine
Components:
Function Name Type
reactant AlaS protein
product AlaS-alanine complex
reactant alanine simple_molecule
AlaS-alanine alaS alaS_mRNA
Name: alaS -> alaS_mRNA
Description: Transcription of transcription unit alaS. Reaction inhibition by AlaS-alanine.
Type: transcription
Pathway Components: alaS - AlaS-alanine -> alaS_mRNA
Components:
Function Name Type
inhibitor AlaS-alanine complex
reactant alaS gene
product alaS_mRNA RNA
alaS_mRNA AlaS
Name: alaS_mRNA -> AlaS
Description: Translation of AlaS
Type: translation
Pathway Components: alaS_mRNA -> AlaS
Components:
Function Name Type
product AlaS protein
reactant alaS_mRNA RNA
AlaS TRNA(ALA) + alanine ALANYL-TRNA(ALA) + diphosphate
Name: TRNA(ALA) + alanine -> ALANYL-TRNA(ALA) + diphosphate
Description: Reaction transforming TRNA(ALA), alanine into ALANYL-TRNA(ALA), diphosphate
Type: state transition
Pathway Components: TRNA(ALA) + alanine - AlaS -> ALANYL-TRNA(ALA) + diphosphate
Components:
Function Name Type
product ALANYL-TRNA(ALA) simple_molecule
catalyzer AlaS protein
reactant TRNA(ALA) simple_molecule
reactant alanine simple_molecule
product diphosphate simple_molecule



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