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AlaS Genetic Sensory Response Unit in Escherichia coli K-12 genome


Gensor Unit Map          
AlaS L-alanine alaS alaS_mRNA AlaS L-alanine


Gensor Unit       
Name: AlaS
Description: Molecular biology level
Detailed: In the presence of alanine AlaS, a dual protein with tRNA aminoacylation enzymatic and DNA binding activities, negatively regulates the expression of genes required to alanyl tRNA acylation process. Under these conditions, AlaS is modified to AlaS-alanine, binds its target sites preventing its own expression.
Less detailed: In the presence of alanine, AlaS negatively regulates the expression of genes needed for tRNA aminocylation.

Physiology level
Detailed: The presence of alanine inhibits the expression of genes related with tRNA aminoacylation process. Under this condition the gene necessary for tRNA-Ala biosynthesis is not expressed.
Less detailed: The presence of alanine inhibits the expression of the gene that mediates tRNA alanine acylation.
Classification:
Groups: GUs related to the metabolism of amino acids
Gene Ontology Terms (GO): biological_process GO:0006419 - alanyl-tRNA aminoacylation
GO:0045892 - negative regulation of transcription, DNA-templated
GO:0006450 - regulation of translational fidelity
GO:0006400 - tRNA modification
cellular_component GO:0005829 - cytosol
GO:0016020 - membrane
molecular_function GO:0002196 - Ser-tRNA(Ala) hydrolase activity
GO:0004813 - alanine-tRNA ligase activity
GO:0016597 - amino acid binding
GO:0002161 - aminoacyl-tRNA editing activity
GO:0042802 - identical protein binding
GO:0001141 - transcriptional repressor activity, bacterial-type RNA polymerase proximal promoter sequence-specific DNA binding
GO:0008270 - zinc ion binding
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Components:
Function Name Type
simple_molecule ALANYL-TRNA(ALA) simple_molecule
protein AlaS protein
complex AlaS-alanine complex
simple_molecule TRNA(ALA) simple_molecule
gene alaS gene
RNA alaS_mRNA RNA
simple_molecule alanine simple_molecule
simple_molecule diphosphate simple_molecule


Reactions       
Showing 4 reactions
alanine + AlaS AlaS-alanine
Name: alanine + AlaS -> AlaS-alanine
Description: Reaction transforming alanine, AlaS into AlaS-alanine
Type: state transition
Pathway Components: alanine + AlaS -> AlaS-alanine
Components:
Function Name Type Evidence Reference(s)
reactant AlaS protein nd nd
product AlaS-alanine complex nd nd
reactant alanine simple_molecule nd nd
AlaS-alanine alaS alaS_mRNA
Name: alaS -> alaS_mRNA
Description: Transcription of transcription unit alaS. Reaction inhibition by AlaS-alanine.
Type: transcription
Pathway Components: alaS - AlaS-alanine -> alaS_mRNA
Components:
Function Name Type Evidence Reference(s)
inhibitor AlaS-alanine complex nd nd
reactant alaS gene nd nd
product alaS_mRNA RNA nd nd
alaS_mRNA AlaS
Name: alaS_mRNA -> AlaS
Description: Translation of AlaS
Type: translation
Pathway Components: alaS_mRNA -> AlaS
Components:
Function Name Type Evidence Reference(s)
product AlaS protein nd nd
reactant alaS_mRNA RNA nd nd
AlaS TRNA(ALA) + alanine ALANYL-TRNA(ALA) + diphosphate
Name: TRNA(ALA) + alanine -> ALANYL-TRNA(ALA) + diphosphate
Description: Reaction transforming TRNA(ALA), alanine into ALANYL-TRNA(ALA), diphosphate
Type: state transition
Pathway Components: TRNA(ALA) + alanine - AlaS -> ALANYL-TRNA(ALA) + diphosphate
Components:
Function Name Type Evidence Reference(s)
product ALANYL-TRNA(ALA) simple_molecule nd nd
catalyzer AlaS protein nd nd
reactant TRNA(ALA) simple_molecule nd nd
reactant alanine simple_molecule nd nd
product diphosphate simple_molecule nd nd



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