RegulonDB RegulonDB 10.6.3: Operon Form
   

araC operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: araC
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: araC
Synonym(s): OP00004
Gene(s): araC   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Lee NL., et al., 1981
[2] Miyada CG., et al., 1984
Promoter
Name: araCp
+1: 70241
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 146
Sequence: caatgtggacttttctgccgtgattatagacacttttgttacgcgtttttgtcatggcttTggtcccgctttgttacagaa
                           -35                       -10    +1                   
Evidence: [TIM]
Reference(s): [3] Wallace RG., et al., 1980
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote AraC dual araCp 70131 70147 -102.0 ccgctaatctTATGGATAAAAATGCTAtggcatagca nd [BPP], [GEA], [HIBSCS], [SM] [1], [4], [5], [8], [9], [10]
remote AraC-arabinose dual araCp 70131 70147 -102.0 ccgctaatctTATGGATAAAAATGCTAtggcatagca nd [BPP], [GEA], [HIBSCS], [SM] [1], [4], [5], [8], [9], [10]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote AraC-arabinose activator araCp 70110 70126 -123.0 ataaaaagcgTCAGGTAGGATCCGCTAatcttatgga nd [BPP], [GEA], [HIBSCS], [SM] [4], [5], [6], [7], [8], [9]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal AraC-arabinose repressor araCp 70184 70200 -49.0 aaataatcaaTGTGGACTTTTCTGCCGtgattataga nd [BCE], [GEA], [HIBSCS], [SM] [1], [5], [15]
proximal AraC-arabinose repressor araCp 70205 70221 -28.0 ctgccgtgatTATAGACACTTTTGTTAcgcgtttttg nd [BCE], [GEA], [HIBSCS], [SM] [1], [5], [15]
remote AraC repressor araCp 70342 70358 110.0 tctgatgcaaTATGGACAATTGGTTTCttctctgaat nd [BCE], [BPP], [HIBSCS], [SM] [1], [5], [6], [8], [10], [17]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator araCp 70158 70179 -72.5 tggcatagcaAAGTGTGACGCCGTGCAAATAAtcaatgtgga nd [BPP], [GEA], [HIBSCS], [SM] [6], [7], [11], [12], [13], [14]
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal XylR-xylose repressor araCp 70195 70213 -37.5 gtggacttttCTGCCGTGATTATAGACACttttgttacg nd [AIBSCS], [BPP], [GEA] [16]
proximal XylR-xylose repressor araCp 70216 70234 -16.5 atagacacttTTGTTACGCGTTTTTGTCAtggctttggt nd [AIBSCS], [BPP], [GEA] [16]




Reference(s)    

 [1] Lee NL., Gielow WO., Wallace RG., 1981, Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia coli., Proc Natl Acad Sci U S A 78(2):752-6

 [2] Miyada CG., Stoltzfus L., Wilcox G., 1984, Regulation of the araC gene of Escherichia coli: catabolite repression, autoregulation, and effect on araBAD expression., Proc Natl Acad Sci U S A 81(13):4120-4

 [3] Wallace RG., Lee N., Fowler AV., 1980, The araC gene of Escherichia coli: transcriptional and translational start-points and complete nucleotide sequence., Gene 12(3-4):179-90

 [4] Bustos SA., Schleif RF., 1993, Functional domains of the AraC protein., Proc Natl Acad Sci U S A 90(12):5638-42

 [5] Gallegos MT., Schleif R., Bairoch A., Hofmann K., Ramos JL., 1997, Arac/XylS family of transcriptional regulators., Microbiol Mol Biol Rev 61(4):393-410

 [6] Huo L., Martin KJ., Schleif R., 1988, Alternative DNA loops regulate the arabinose operon in Escherichia coli., Proc Natl Acad Sci U S A 85(15):5444-8

 [7] Lee N., Francklyn C., Hamilton EP., 1987, Arabinose-induced binding of AraC protein to araI2 activates the araBAD operon promoter., Proc Natl Acad Sci U S A 84(24):8814-8

 [8] Niland P., Huhne R., Muller-Hill B., 1996, How AraC interacts specifically with its target DNAs., J Mol Biol 264(4):667-74

 [9] Reeder T., Schleif R., 1993, AraC protein can activate transcription from only one position and when pointed in only one direction., J Mol Biol 231(2):205-18

 [10] Lee DH., Schleif RF., 1989, In vivo DNA loops in araCBAD: size limits and helical repeat., Proc Natl Acad Sci U S A 86(2):476-80

 [11] Hamilton EP., Lee N., 1988, Three binding sites for AraC protein are required for autoregulation of araC in Escherichia coli., Proc Natl Acad Sci U S A 85(6):1749-53

 [12] Lobell RB., Schleif RF., 1991, AraC-DNA looping: orientation and distance-dependent loop breaking by the cyclic AMP receptor protein., J Mol Biol 218(1):45-54

 [13] Stoltzfus L., Wilcox G., 1989, Effect of mutations in the cyclic AMP receptor protein-binding site on araBAD and araC expression., J Bacteriol 171(2):1178-84

 [14] Zhang X., Schleif R., 1998, Catabolite gene activator protein mutations affecting activity of the araBAD promoter., J Bacteriol 180(2):195-200

 [15] Lee DH., Huo L., Schleif R., 1992, Repression of the araBAD promoter from araO1., J Mol Biol 224(2):335-41

 [16] Koirala S., Wang X., Rao CV., null, Reciprocal Regulation of l-Arabinose and d-Xylose Metabolism in Escherichia coli., J Bacteriol 198(3):386-393

 [17] Seabold RR., Schleif RF., 1998, Apo-AraC actively seeks to loop., J Mol Biol 278(3):529-38


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