RegulonDB RegulonDB 10.6.3: Operon Form
   

galS operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: galS
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: galS
Synonym(s): OP00031
Gene(s): galS   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] Weickert MJ., et al., 1993
Promoter
Name: galSp
+1: 2241710
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 42
Sequence: tttccattgctgtgactcgattcacgaagtcctgtattcagtgctgacaaaatagccgccAgcaagcagtcatttactgca
                     -10                        -35         +1                   
Evidence: [HIPP]
[TIM]
Reference(s): [1] Weickert MJ., et al., 1993
TF binding sites (TFBSs)
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cAMP activator galSp 2241742 2241763 -42.5 ccgtttccatTGCTGTGACTCGATTCACGAAGtcctgtattc nd [AIBSCS], [BCE], [GEA], [HIBSCS] [1], [2], [3], [4]
[GalR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote GalR repressor galSp 2241612 2241627 91.5 ctggcgtctcTGTGGCAACGGTTTCCcgggtgctca nd [GEA], [HIBSCS] [1], [2], [3]
proximal GalR repressor galSp 2241764 2241779 -61.5 aacaaaacggCTGTAACCGTTTCCATtgctgtgact nd [GEA], [HIBSCS] [1], [3]
[GalS,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote GalS repressor galSp 2241611 2241626 92.5 tggcgtctctGTGGCAACGGTTTCCCgggtgctcaa nd [BCE], [GEA] [1], [2]
proximal GalS2 repressor galSp 2241764 2241779 -61.5 aacaaaacggCTGTAACCGTTTCCATtgctgtgact nd [BCE], [GEA] [1], [2]
Note(s): 2Based on the effect of various concentrations of GalS on the gal regulon promoter and mathematical models, it was determined that GalS has higher affinity for galSOE, which is at position -61.5 on the galS promoter Semsey S, Krishna S, Erdossy J, Horváth P, Orosz L, Sneppen K, Adhya S,2009 This effect is referred to as dominant negative autoregulation Semsey S, Krishna S, Erdossy J, Horváth P, Orosz L, Sneppen K, Adhya S,20091Based on the effect of various concentrations of GalS on the gal regulon promoter and mathematical models, it was determined that GalS has higher affinity for galSOE, which is at position -61.5 on the galS promoter Semsey S, Krishna S, Erdossy J, Horváth P, Orosz L, Sneppen K, Adhya S,2009 This effect is referred to as dominant negative autoregulation Semsey S, Krishna S, Erdossy J, Horváth P, Orosz L, Sneppen K, Adhya S,2009


Evidence    

 [HIPP] Human inference of promoter position

 [TIM] Transcription initiation mapping

 [AIBSCS] Automated inference based on similarity to consensus sequences

 [BCE] Binding of cellular extracts

 [GEA] Gene expression analysis

 [HIBSCS] Human inference based on similarity to consensus sequences


Reference(s)    

 [1] Weickert MJ., Adhya S., 1993, Control of transcription of gal repressor and isorepressor genes in Escherichia coli., J Bacteriol 175(1):251-8

 [2] Semsey S., Krishna S., Sneppen K., Adhya S., 2007, Signal integration in the galactose network of Escherichia coli., Mol Microbiol 65(2):465-76

 [3] Weickert MJ., Adhya S., 1993, The galactose regulon of Escherichia coli., Mol Microbiol 10(2):245-51

 [4] Zheng D., Constantinidou C., Hobman JL., Minchin SD., 2004, Identification of the CRP regulon using in vitro and in vivo transcriptional profiling., Nucleic Acids Res 32(19):5874-93

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