RegulonDB RegulonDB 10.6.3: Operon Form

ilvY operon and associated TUs in Escherichia coli K-12 genome

Name: ilvY
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: ilvY
Synonym(s): OP00041
Gene(s): ilvY   Genome Browser M3D Gene expression COLOMBOS
Note(s): DNase I footprint analysis |CITS: [3062177][9556617]| and DNA binding assay results |CITS: [3062177]| showed that IlvY binds in tandem upstream of the transcription start site of the gene ilvY. Wey et al. proposed that this regulator binds to regions of 28 bp that contain inverted repeat motifs of 21 bp |CITS: [3062177]|. Using a new methodology based on analysis of orthologous sequences, the curator has identified the consensus sequence of IlvY (aTTGCaAatattGCAAT) of 17 bp, which is similar to the consensus sequence identified in the RegPrecise database (aTTGCaaaatttGCAAt).
Reference(s): [1] Sameshima JH., et al., 1989
[2] Warne SR., et al., 1990
[3] Wek RC., et al., 1986
Name: ilvYp
+1: 3957867
Sigma Factor: Sigma70, Sigma38
Distance from start of the gene: 47
Sequence: gtttgcgagattgaattcactatatgacaggaaatttattgcggaaattgatatattcacAacgtcacattgcaatttttg
                     -10               -35                  +1                   
Evidence: [HIPP]
Reference(s): [4] Opel ML., et al., 2001
[5] Rhee KY., et al., 1999
[3] Wek RC., et al., 1986
Type: rho-independent
Sequence: tagggaaaaaTGCCTGATAGCGCTTCGCTTATCAGGCctacccgcgc
Reference(s): [1] Sameshima JH., et al., 1989
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal IlvY repressor ilvYp 3957843 3957860 17.0 cacaacgtcaCATTGCAATTTTTGCAACgtcaacatcg nd [BCE], [BPP], [GEA], [HIBSCS], [IC] [6], [7], [8], [9], [10]
proximal IlvY repressor ilvYp 3957874 3957891 -15.0 acaggaaattTATTGCGGAAATTGATATattcacaacg nd [BCE], [BPP], [GEA], [HIBSCS], [IC] [6], [7]


 [1] Sameshima JH., Wek RC., Hatfield GW., 1989, Overlapping transcription and termination of the convergent ilvA and ilvY genes of Escherichia coli., J Biol Chem 264(2):1224-31

 [2] Warne SR., Varley JM., Boulnois GJ., Norton MG., 1990, Identification and characterization of a gene that controls colony morphology and auto-aggregation in Escherichia coli K12., J Gen Microbiol 136 ( Pt 3):455-62

 [3] Wek RC., Hatfield GW., 1986, Nucleotide sequence and in vivo expression of the ilvY and ilvC genes in Escherichia coli K12. Transcription from divergent overlapping promoters., J Biol Chem 261(5):2441-50

 [4] Opel ML., Hatfield GW., 2001, DNA supercoiling-dependent transcriptional coupling between the divergently transcribed promoters of the ilvYC operon of Escherichia coli is proportional to promoter strengths and transcript lengths., Mol Microbiol 39(1):191-8

 [5] Rhee KY., Opel M., Ito E., Hung S., Arfin SM., Hatfield GW., 1999, Transcriptional coupling between the divergent promoters of a prototypic LysR-type regulatory system, the ilvYC operon of Escherichia coli., Proc Natl Acad Sci U S A 96(25):14294-9

 [6] Rhee KY., Senear DF., Hatfield GW., 1998, Activation of gene expression by a ligand-induced conformational change of a protein-DNA complex., J Biol Chem 273(18):11257-66

 [7] Wek RC., Hatfield GW., 1988, Transcriptional activation at adjacent operators in the divergent-overlapping ilvY and ilvC promoters of Escherichia coli., J Mol Biol 203(3):643-63

 [8] Biel AJ., Umbarger HE., 1981, Mutations in the ilvY gene of Escherichia coli K-12 that cause constitutive expression of ilvC., J Bacteriol 146(2):718-24

 [9] Pledger WJ., Umbarger HE., 1973, Isoleucine and valine metabolism in Escherichia coli. XXII. A pleiotropic mutation affecting induction of isomeroreductase activity., J Bacteriol 114(1):195-207

 [10] Watson MD., Wild J., Umbarger HE., 1979, Positive control of ilvC expression in Escherichia coli K-12; identification and mapping of regulatory gene ilvY., J Bacteriol 139(3):1014-20