RegulonDB RegulonDB 10.8: Operon Form
   

moaABCDE operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: moaABCDE
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: moaABCDE
Gene(s): moaA, moaB, moaC, moaD, moaE   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] McNicholas PM., et al., 1997
[2] Rivers SL., et al., 1993
Promoter
Name: moaAp1
+1: 816827
Distance from start of the gene: 217
Sequence: gctatgcctccctgatgatgtatatcaaagtaaaaccgccattttcccttattctgtagcGaaatagcacgatcatgacgc
Evidence: [CV(RS-EPT-CBR)]
[CV(TIM)]
[RS-EPT-CBR]
[TIM]
Reference(s): [3] Anderson LA., et al., 2000
[4] Salgado H, et al., 2012
TF binding sites (TFBSs)
[CueR,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote CueR-Cu+ repressor moaAp1 817019 817037 202.0 aaatgacttcGCCTCCCGTATCTGGAAAGgtgtacatgg nd [APIORCISFBSCS], [CV(GEA)], [GEA] [6]
[FNR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal FNR1 activator moaAp1 816781 816794 -39.5 tgcctccctgATGATGTATATCAAagtaaaaccg nd [APIORCISFBSCS], [CV(GEA)], [GEA] [3], [5]
Note(s): 1The expression of the moaABCDE operon is decreased in a FNR mutant; however, when the region containing the FNR binding site is removed, the expression of the operon is enhanced in a FNR mutant Zupok A,2019.1The expression of the moaABCDE operon is decreased in a FNR mutant; however, when the region containing the FNR binding site is removed, the expression of the operon is enhanced in a FNR mutant Zupok A,2019.


Transcription unit          
Name: moaABCDE
Gene(s): moaA, moaB, moaC, moaD, moaE   Genome Browser M3D Gene expression COLOMBOS
Reference(s): [1] McNicholas PM., et al., 1997
[2] Rivers SL., et al., 1993
Promoter
Name: moaAp2
+1: 816914
Distance from start of the gene: 130
Sequence: catgattacatagcgaaagtgtggatggtaaaaatctcatttacacgctagtatcggcatAaccactaaacactctagcct
Evidence: [CV(RS-EPT-CBR)]
[CV(TIM)]
[RS-EPT-CBR]
[TIM]
Reference(s): [3] Anderson LA., et al., 2000
[4] Salgado H, et al., 2012
TF binding sites (TFBSs)
[ModE,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal ModE-MoO42-1 activator moaAp2 816845 816868 -57.5 acgatcatgaCGCTATATACATGATTACATAGCGaaagtgtgga nd [BPP], [GEA] [1]
Note(s): 1Based on MoaA gel shift assays and moaA-lacZ operon fusions , molybdate had no effect on ModE binding to moaAp2 McNicholas PM,1997.1Based on MoaA gel shift assays and moaA-lacZ operon fusions , molybdate had no effect on ModE binding to moaAp2 McNicholas PM,1997.


Transcription unit       
Name: moaBCDE
Gene(s): moaB, moaC, moaD, moaE   Genome Browser M3D Gene expression COLOMBOS
Promoter
Name: moaBp
+1: 817977
Distance from start of the gene: 78
Sequence: cagcaacaggcgctggaagcgcgtatttcagcggcgctgcgggagaagaaacagacccatTtcctgcatcaaaacaacacc
Evidence: [CV(RS-EPT-CBR)]
[CV(TIM)]
[RS-EPT-CBR]
[TIM]
Reference(s): [4] Salgado H, et al., 2012
[6] Yamamoto K., et al., 2005


Evidence    

 [CV(RS-EPT-CBR)] cross validation(RS-EPT-CBR)

 [CV(TIM)] cross validation(TIM)

 [RS-EPT-CBR] RNA-seq using two enrichment strategies for primary transcripts and consistent biological replicates

 [TIM] Transcription initiation mapping

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.

 [CV(GEA)] cross validation(GEA)

 [GEA] Gene expression analysis

 [BPP] Binding of purified proteins


Reference(s)    

 [1] McNicholas PM., Rech SA., Gunsalus RP., 1997, Characterization of the ModE DNA-binding sites in the control regions of modABCD and moaABCDE of Escherichia coli., Mol Microbiol 23(3):515-24

 [2] Rivers SL., McNairn E., Blasco F., Giordano G., Boxer DH., 1993, Molecular genetic analysis of the moa operon of Escherichia coli K-12 required for molybdenum cofactor biosynthesis., Mol Microbiol 8(6):1071-81

 [3] Anderson LA., McNairn E., Lubke T., Pau RN., Boxer DH., Leubke T., 2000, ModE-dependent molybdate regulation of the molybdenum cofactor operon moa in Escherichia coli., J Bacteriol 182(24):7035-43

 [4] Salgado H, Peralta-Gil M, Gama-Castro S, Santos-Zavaleta A, Muñiz-Rascado L, García-Sotelo JS, Weiss V, Solano-Lira H, Martínez-Flores I, Medina-Rivera A, Salgado-Osorio G, Alquicira-Hernández S, Alquicira-Hernández K, López-Fuentes A, Porrón-Sotelo L, Huerta AM, Bonavides-Martínez C, Balderas-Martínez YI, Pannier L, Olvera M, Labastida A, Jiménez-Jacinto V, Vega-Alvarado L, Del Moral-Chávez V, Hernández-Alvarez A, Morett E, Collado-Vides J., 2012, RegulonDB v8.0: omics data sets, evolutionary conservation, regulatory phrases, cross-validated gold standards and more., Nucleic Acids Res.

 [5] Zupok A., Gorka M., Siemiatkowska B., Skirycz A., Leimkuhler S., 2019, Iron-Dependent Regulation of Molybdenum Cofactor Biosynthesis Genes in Escherichia coli., J Bacteriol 201(17)

 [6] Yamamoto K., Ishihama A., 2005, Transcriptional response of Escherichia coli to external copper., Mol Microbiol 56(1):215-27

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