RegulonDB RegulonDB 10.10: Operon Form
   

dctA operon and associated TUs in Escherichia coli K-12 genome




Operon      
Name: dctA
This page displays every known transcription unit of this operon and their known regulation.


Transcription unit          
Name: dctA
Gene(s): dctA   Genome Browser M3D Gene expression COLOMBOS
Note(s): Under NlpE outer membrane lipoprotein overexpression, the transcription of the dctA gene is decreased Raivio TL,2013
Evidence: [LTED] Length of transcript experimentally determined
Reference(s): [1] Davies SJ., et al., 1999
Promoter
Name: dctAp
+1: 3683498
Sigma Factor: Sigma70 Sigmulon
Distance from start of the gene: 51
Sequence: ttgtttcaattatgatccaggtacatttctgtgatgttgtctgggtgttattttaaggccGcaggtaccccataaccttac
                          -35                    -10        +1                   
Evidence: [ICWHO]
[TIM]
Reference(s): [1] Davies SJ., et al., 1999
[2] Huerta AM., et al., 2003
TF binding sites (TFBSs)
[ArcA,-] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
nd ArcA-phosphorylated repressor dctAp nd nd nd nd nd [GEA], [BPP] [1]
[CRP,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal CRP-cyclic-AMP activator dctAp 3683569 3683590 -81.5 aagtgctggtTTGTGCGAGCCAGCTCAAACTTtttaaccttt nd [GEA], [APIORCISFBSCS], [BPP] [1], [6]
[DcuR,+] Phrase
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence (Confirmed, Strong, Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
remote DcuR-phosphorylated activator dctAp 3683596 3683612 -106.0 aaactgattaCAAAACTTTAAAAAGTGctggtttgtg nd [GEA], [BPP] [1], [3], [4], [5], [6]


Evidence    

 [ICWHO] Inferred computationally without human oversight

 [TIM] Transcription initiation mapping

 [GEA] Gene expression analysis

 [BPP] Binding of purified proteins

 [APIORCISFBSCS] A person inferred or reviewed a computer inference of sequence function based on similarity to a consensus sequence.


Reference(s)    

 [1] Davies SJ., Golby P., Omrani D., Broad SA., Harrington VL., Guest JR., Kelly DJ., Andrews SC., 1999, Inactivation and regulation of the aerobic C(4)-dicarboxylate transport (dctA) gene of Escherichia coli., J Bacteriol 181(18):5624-35

 [2] Huerta AM., Collado-Vides J., 2003, Sigma70 promoters in Escherichia coli: specific transcription in dense regions of overlapping promoter-like signals., J Mol Biol 333(2):261-78

 [3] Abo-Amer AE., Munn J., Jackson K., Aktas M., Golby P., Kelly DJ., Andrews SC., 2004, DNA interaction and phosphotransfer of the C4-dicarboxylate-responsive DcuS-DcuR two-component regulatory system from Escherichia coli., J Bacteriol 186(6):1879-89

 [4] Golby P., Davies S., Kelly DJ., Guest JR., Andrews SC., 1999, Identification and characterization of a two-component sensor-kinase and response-regulator system (DcuS-DcuR) controlling gene expression in response to C4-dicarboxylates in Escherichia coli., J Bacteriol 181(4):1238-48

 [5] Janausch IG., Garcia-Moreno I., Lehnen D., Zeuner Y., Unden G., 2004, Phosphorylation and DNA binding of the regulator DcuR of the fumarate-responsive two-component system DcuSR of Escherichia coli., Microbiology 150(Pt 4):877-83

 [6] Surmann K., Stopp M., Worner S., Dhople VM., Volker U., Unden G., Hammer E., 2020, Fumarate dependent protein composition under aerobic and anaerobic growth conditions in Escherichia coli., J Proteomics 212:103583

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