RegulonDB RegulonDB 11.1: Operon Form

flxA operon and associated TUs in Escherichia coli K-12 genome

Name: flxA
This page displays every known transcription unit of this operon and their known regulation.

Transcription unit          
Name: flxA
Gene(s): flxA   Genome Browser M3D Gene expression COLOMBOS
Evidence: [EXP-IDA-BOUNDARIES-DEFINED] Boundaries of transcription experimentally identified
Reference(s): [1] Ide N., et al., 1997
Name: flxAp
+1: 1646388
Sigma Factor: Sigma28 Sigmulon
Distance from start of the gene: 17
Sequence: aacaattaatgacacaagaaatacgattaaagatttttttgtgcatgccgatagtgctttTttaaaaggagaaatctatgt
                            -35                -10          +1                   
Note(s): The sequence of this promoter has been defined, by computational analyses, from the consensus sequence of the σ28F) promoters: TAAAGTTT in the -35 box and GCCGATAA in the -10 box Park K,2001. Therefore, we assigned a putative transcription start site for this promoter based on the observation that the majority of the σ28 promoters, determined experimentally, present an important -10 motif (5'-CGA-3') at the positions -12 to -10 and a distance of 6 nucleotides between the transcription start site and the -10 box Yu HH, Di Russo EG, Rounds MA, Tan M,2006. Yu HH,2006. Park K,2001.
Ide et al. showed in 1997 that transcription of flxA is highly dependent on alternative factor σF Ide N,1997. The transcription expression level was examined in a fliA mutant background.
Evidence: [COMP-AINF]
Reference(s): [2] Huerta AM., et al., 2003
[1] Ide N., et al., 1997
[3] Park K., et al., 2001
[4] Zhao K., et al., 2007
TF binding sites (TFBSs)
Type Transcription factor Function Promoter Binding Sites Growth Conditions Evidence Confidence level (C: Confirmed, S: Strong, W: Weak) Reference(s)
LeftPos RightPos Central Rel-Pos Sequence
proximal Fis activator flxAp 1646309 1646323 -72.0 agcccgcgagGAAAACAATCTGAACatcaaacaat nd [EXP-IEP-GENE-EXPRESSION-ANALYSIS], [COMP-AINF-SIMILAR-TO-CONSENSUS] W [5]